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Volume 17, Issue 11 (November 2019)
IJRM 2019, 17(11): 789-798 |
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Omidi M, Ahangarpour A, Mard S A, Khorsandi L. The effects of myricitrin and vitamin E against reproductive changes induced by D-galactose as an aging model in female mice: An experimental study. IJRM 2019; 17 (11) :789-798
URL: http://ijrm.ir/article-1-1719-en.html
URL: http://ijrm.ir/article-1-1719-en.html
1- Department of Physiology, Faculty of Medicine, Student Research Committee, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.
2- Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , akramahangarpour@gmail.com
3- 2Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Anatomical Sciences, Faculty of Medicine, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2- Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , akramahangarpour@gmail.com
3- 2Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Anatomical Sciences, Faculty of Medicine, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Abstract: (2499 Views)
Abstract
Background: Aging is accompanied by decreasing general function in the cells and tissues. D-galactose (D-gal) induces aging and plays a role in the pathogenesis of it. Myricitrin is a plant-derived antioxidant.
Objective: The present study was performed to evaluate the effects of myricitrin on antioxidant defense, sex hormone levels, uterus, and ovarian histology in D-gal-induced aging female mouse model.
Materials and Methods: In this experimental study, 72 female adult NMRI mice, weighing 30-35 gr, 3-4 months old, were randomly divided into six groups (n = 12/each): (I) Control (vehicle; normal saline), (II) D-gal at 500 mg/kg/d for 45 days, (III-V) D-gal + myricitrin-treated groups (these groups received myricitrin at 5, 10, and 20 mg/kg/d, and (VI) D-gal + 100 mg/kg/d vitamin E orally for the last 28 days. The antioxidant indices were done on the basis of colorimetric method, and sex hormone levels were measured by using enzyme-linked immunosorbent assay kits. Histological assessment of the uterus and ovaries were also evaluated.
Results: D-gal impaired the estrous cycle, also degenerative changes occur in the ovarian follicles and damage to the uterus and ovarian tissue occurs. In D-gal group, the level of sex hormones (p =0.03) and the total antioxidant capacity (p = 0.002) decreased, while the level of malondialdehyde and gonadotropins increased (p = 0.03). Myricitrin at lower doses and vitamin E ameliorated the D-gal effects.
Conclusion: These findings suggest that myricitrin at low doses can effectively prevent D-gal-induced oxidation and aging in mice. The effect of myricitrin was equivalent and sometimes better than vitamin E.
Background: Aging is accompanied by decreasing general function in the cells and tissues. D-galactose (D-gal) induces aging and plays a role in the pathogenesis of it. Myricitrin is a plant-derived antioxidant.
Objective: The present study was performed to evaluate the effects of myricitrin on antioxidant defense, sex hormone levels, uterus, and ovarian histology in D-gal-induced aging female mouse model.
Materials and Methods: In this experimental study, 72 female adult NMRI mice, weighing 30-35 gr, 3-4 months old, were randomly divided into six groups (n = 12/each): (I) Control (vehicle; normal saline), (II) D-gal at 500 mg/kg/d for 45 days, (III-V) D-gal + myricitrin-treated groups (these groups received myricitrin at 5, 10, and 20 mg/kg/d, and (VI) D-gal + 100 mg/kg/d vitamin E orally for the last 28 days. The antioxidant indices were done on the basis of colorimetric method, and sex hormone levels were measured by using enzyme-linked immunosorbent assay kits. Histological assessment of the uterus and ovaries were also evaluated.
Results: D-gal impaired the estrous cycle, also degenerative changes occur in the ovarian follicles and damage to the uterus and ovarian tissue occurs. In D-gal group, the level of sex hormones (p =0.03) and the total antioxidant capacity (p = 0.002) decreased, while the level of malondialdehyde and gonadotropins increased (p = 0.03). Myricitrin at lower doses and vitamin E ameliorated the D-gal effects.
Conclusion: These findings suggest that myricitrin at low doses can effectively prevent D-gal-induced oxidation and aging in mice. The effect of myricitrin was equivalent and sometimes better than vitamin E.
Type of Study: Original Article |
References
1. Wang S, He G, Chen M, Zuo T, Xu W, Liu X. The role of antioxidant enzymes in the ovaries. Oxid Med Cell Longev. 2017; 2017: 4371714. [DOI:10.1155/2017/4371714] [PMID] [PMCID]
2. Li YJ, Han Z, Ge L, Zhou CJ, Zhao YF, Wang DH, et al. C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice. Oncotarget 2016; 7: 17393-17409. [DOI:10.18632/oncotarget.8165]
3. Zhao FF, Zhou YZ, Gao L, Qin XM, Du GH. Advances in the study of the rat model of aging induced by D-galactose. Yao Xue Xue Bao 2017; 52: 347-354.
4. Ahangarpour A, Najimi SA, Farbood Y. Effects of Vitex agnus-castus fruit on sex hormones and antioxidant indices in a d-galactose-induced aging female mouse model. J Chin Med Assoc 2016; 79: 589-596. [DOI:10.1016/j.jcma.2016.05.006] [PMID]
5. Chen H, Long Y, Guo L. Antiaging Effect of inula britannica on aging mouse model induced by d-galactose. Evid Based Complement Alternat Med 2016; 2016: 1-8. [DOI:10.1155/2016/6049083] [PMID] [PMCID]
6. Chen W, Feng L, Shen Y, Su H, Li Y, Zhuang J, et al. Myricitrin inhibits acrylamide-mediated cytotoxicity in human Caco-2 cells by preventing oxidative stress. Biomed Res Int 2013; 2013: 1-7. [DOI:10.1155/2013/839761] [PMID] [PMCID]
7. Ahangarpour A, Lamoochi Z, Fathi Moghaddam H, Mansouri SM. Effects of portulaca oleracea ethanolic extract on reproductive system of aging female mice. Int J Reprod BioMed 2016; 14: 205-212. [DOI:10.29252/ijrm.14.3.205]
8. Huang Q, Gao B, Wang L, Hu YQ, Lu WG, Yang L, et al. Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines. Toxicol Appl Pharmacol 2014; 280: 550-560. [DOI:10.1016/j.taap.2014.08.004] [PMID]
9. Li J, Zhang M, Ma J. Myricitrin inhibits PDGF-BB-stimulated vascular smooth muscle cell proliferation and migration through suppressing PDGFRβ/Akt/Erk signaling. Int J Clin Exp Med 2015; 8: 21715-21723.
10. Ahangarpour A, Oroojan AA, Khorsandi L, Kouchak M, Badavi M. Solid lipid nanoparticles of myricitrin have antioxidant and antidiabetic effects on streptozotocin-nicotinamide-induced diabetic model and myotube cell of male mouse. Oxid Med Cell Longev 2018; 2018: 7496936. [DOI:10.1155/2018/7496936] [PMID] [PMCID]
11. Kassem ME, Ibrahim LF, Hussein SR, El-Sharawy R, El-Ansari MA, Hassanane MM, et al. Myricitrin and bioactive extract of Albizia amara leaves: DNA protection and modulation of fertility and antioxidant-related genes expression. Pharm Biol 2016; 54: 2404-2409. [DOI:10.3109/13880209.2016.1158285] [PMID]
12. Soleimani Mehranjani M, Noorafshan A, Hamta A, Momeni HR, Abnosi MH, Mahmoodi M, et al. Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: A stereological study. Iran J Reprod Med 2010; 8: 1-9.
13. Ekambaram G, Sampath Kumar SK, Joseph LD. Comparative study on the estimation of estrous cycle in mice by visual and vaginal lavage method. J Clin Diagn Res 2017; 11: AC05-AC07. [DOI:10.7860/JCDR/2017/23977.9148] [PMID] [PMCID]
14. Park JH, Choi TS. Polycystic ovary syndrome (PCOS)-like phenotypes in the d-galactose-induced aging mouse model. Biochem Biophys Res Commun 2012; 427: 701-704. [DOI:10.1016/j.bbrc.2012.09.099] [PMID]
15. Budni J, Pacheco R, da Silva S, Garcez ML, Mina F, Bellettini-Santos T, et al. Oral administration of d-galactose induces cognitive impairments and oxidative damage in rats. Behav Brain Res 2016; 302: 35-43. [DOI:10.1016/j.bbr.2015.12.041] [PMID]
16. Ahangarpour A, Oroojan AA, Heidari H. Effects of exendin-4 on male reproductive parameters of D-galactose induced aging mouse model. World J Mens Health 2014; 32: 176-183. [DOI:10.5534/wjmh.2014.32.3.176] [PMID] [PMCID]
17. Sekhon LH, Gupta S, Kim Y, Agarwal A. Female infertility and antioxidants. Current Women's Health Reviews 2010; 6: 84-95. [DOI:10.2174/157340410791321381]
18. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol 2012; 10: 49-80. [DOI:10.1186/1477-7827-10-49] [PMID] [PMCID]
19. Behrman HR, Kodaman PH, Preston SL, Gao S. Oxidative stress and the ovary. J Soc Gynecol Investig 2001; 8 (Suppl.): S40-S42. [DOI:10.1177/1071557601008001S13]
20. Kannappan R, Gupta SC, Kim JH, Aggarwal BB. Tocotrienols fight cancer by targeting multiple cell signaling pathways. Genes Nutr 2012; 7: 43-52. [DOI:10.1007/s12263-011-0220-3] [PMID] [PMCID]
21. Mohd Mutalip SS, Ab-Rahim S, Rajikin MH. Vitamin E as an antioxidant in female reproductive health. Antioxidants 2018; 7: 1-22. [DOI:10.3390/antiox7020022] [PMID] [PMCID]
22. Bahadori MH, Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF. Level of vitamin e in follicular fluid and serum and oocyte morphology and embryo quality in patients undergoing ivf treatment. J Family Reprod Health 2017; 11: 74-81.
23. Cicek N, Eryilmaz OG, Sarikaya E, Gulerman C, Genc Y. Vitamin E effect on controlled ovarian stimulation of unexplained infertile women. J Assist Reprod Genet 2012; 29: 325-328. [DOI:10.1007/s10815-012-9714-1] [PMID] [PMCID]
24. Ahangarpour A, Oroojan AA, Khorsandi L, Kouchak M, Badavi M. Antioxidant effect of myricitrin on hyperglycemia-induced oxidative stress in C2C12 cell. Cell Stress Chaperones 2018; 23: 773-781. [DOI:10.1007/s12192-018-0888-z] [PMID] [PMCID]
25. Yan Z, Dai Y, Fu H, Zheng Y, Bao D, Yin Y, et al. Curcumin exerts a protective effect against premature ovarian failure in mice. J Mol Endocrinol 2018; 60: 261-271. [DOI:10.1530/JME-17-0214] [PMID] [PMCID]
26. Leeners B, Geary N, Tobler PN, Asarian L. Ovarian hormones and obesity. Hum Reprod Update 2017; 23: 300-321. [DOI:10.1093/humupd/dmw045] [PMID] [PMCID]
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