Volume 8, Issue 4 (7-2010)                   IJRM 2010, 8(4): 157-160 | Back to browse issues page

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Aflatoonian A, Mashayekhy M, Mohamadian F, Mansoori Moghaddam F. The correlation between follicular fluid anti-mullerian hormone levels and fertilization and embryo quality in ART cycles. IJRM 2010; 8 (4) :157-160
URL: http://ijrm.ir/article-1-198-en.html
The correlation between follicular fluid anti-mullerian hormone levels and fertilization and embryo quality in ART cycles
Abbas Aflatoonian1 , Mehri Mashayekhy * 2, Farnaz Mohamadian1 , Fatemeh Mansoori Moghaddam1
1- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Dr.Mashayekhy@yahoo.com
Abstract:   (2267 Views)
Background: Determination of oocyte and embryo quality are one of the most important goals in IVF. Anti-mullerian hormone (AMH) is secreted by the ovarian granulosa cells into blood flow and follicular fluid. Follicular fluid anti-mullerian hormone level is probably a marker of activity of granulose cells. Objective: To evaluate whether high level of follicular fluid anti-mullerian hormone level is related to success of fertilization and better embryo quality. Materials and Methods: 62 women, whose follicular fluid sample was obtained from a single follicle in each patient, underwent IVF with GnRH-agonist long protocol. Based on oocyte fertilization, the patients were divided into fertilized group (n=42) and non-fertilized group (n=20). FF AMH levels were measured in both groups and the quality of embryos was determined in fertilized group.
Results: Median of FF AMH level in fertilized group was higher than that in non-fertilized group (5.7ng/ml v.s. 2.7ng/ml) and a statistically significant difference was observed between the two groups. There was a significant difference between FF AMH level and scores of embryos (p<0.001). The medians levels of FF AMH were 6.7ng/ml in good quality embryos and 3.80ng/ml in fair quality embryos.
Conclusion: Our results indicate that FF AMH level has positive correlation with fertilization and embryo quality; therefore, it can be considered as a marker of IVF outcome.
Keywords: Anti-mullerian hormone, Follicular fluid, Fertilization, Embryo quality, In-vitro fertilization.
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Type of Study: Original Article |
References
1. Revelli A, Delle Piane L, Casano S, Molinari E, Massobrio M, Rinaudo P. Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics. Reprod Biol Endocrinol 2009; 7: 40. [DOI:10.1186/1477-7827-7-40]
2. Knight P, Glister C. TGF-{beta} superfamily members and ovarian follicle development. Reproduction 2006; 132: 191-206. [DOI:10.1530/rep.1.01074]
3. La Marca A, Sighinolfi G, Radi D, Argento C, Baraldi E, Artenisio AC, et al. Anti-Mullerian hormone (AMH) as a predictive marker in assisted reproductive technology (ART). Hum Reprod Update 2010; 16: 113-130. [DOI:10.1093/humupd/dmp036]
4. Gruijters MJ VJ, Durlinger AL, Themmen AP. Anti-Müllerian hormone and its role in ovarian function. Mol Cell Endocrinol 2003; 211: 85-90. [DOI:10.1016/j.mce.2003.09.024]
5. Das M, Gillott DJ, Saridogan E, Djahanbakhch O. Anti-Mullerian hormone is increased in follicular fluid from unstimulated ovaries in women with polycystic ovary syndrome. Hum Reprod 2008; 23: 2122-2126. [DOI:10.1093/humrep/den185]
6. La Marca A, Broekmans FJ, Volpe A, Fauser BC, Macklon NS. Anti-Mullerian hormone (AMH): what do we still need to know? Hum Reprod 2009; 24: 2264-2275. [DOI:10.1093/humrep/dep210]
7. Weenen C, Laven JS, Von Bergh AR, Cranfield M, Groome NP, Visser JA, et al. Anti-Mullerian hormone expression pattern in the human ovary: potential implications for initial and cyclic follicle recruitment. Mol Hum Reprod 2004; 10: 77-83. [DOI:10.1093/molehr/gah015]
8. Vigier B, Picard JY, Tran D, Legeai L, Josso N. Production of anti-Mullerian hormone: another homology between Sertoli and granulosa cells. Endocrinology 1984; 114: 1315-1320. [DOI:10.1210/endo-114-4-1315]
9. Eldar-Geva T, Ben-Chetrit A, Spitz IM, Rabinowitz R, Markowitz E, Mimoni T, et al. Dynamic assays of inhibin B, anti-Mullerian hormone and estradiol following FSH stimulation and ovarian ultrasonography as predictors of IVF outcome. Hum Reprod 2005; 20: 3178-3183. [DOI:10.1093/humrep/dei203]
10. Fanchin R, Taieb J, Lozano DH, Ducot B, Frydman R, Bouyer J. High reproducibility of serum anti-Mullerian hormone measurements suggests a multi-staged follicular secretion and strengthens its role in the assessment of ovarian follicular status. Hum Reprod 2005; 20: 923-927. [DOI:10.1093/humrep/deh688]
11. Ebner T, Sommergruber M, Moser M, Shebl O, Schreier-Lechner E, Tews G. Basal level of anti-Mullerian hormone is associated with oocyte quality in stimulated cycles. Hum Reprod 2006; 21: 2022-2026. [DOI:10.1093/humrep/del127]
12. Silberstein T, MacLaughlin DT, Shai I, Trimarchi JR, Lambert-Messerlian G, Seifer DB, et al. Mullerian inhibiting substance levels at the time of HCG administration in IVF cycles predict both ovarian reserve and embryo morphology. Hum Reprod 2006; 21: 159-163. [DOI:10.1093/humrep/dei270]
13. Feyereisen E, Mendez Lozano DH, Taieb J, Hesters L, Frydman R, Fanchin R. Anti-Mullerian hormone: clinical insights into a promising biomarker of ovarian follicular status. Reprod Biomed Online 2006; 12: 695-703. [DOI:10.1016/S1472-6483(10)61081-4]
14. Bath LE, Wallace WH, Shaw MP, Fitzpatrick C, Anderson RA. Depletion of ovarian reserve in young women after treatment for cancer in childhood: detection by anti-Mullerian hormone, inhibin B and ovarian ultrasound. Hum Reprod 2003; 18: 2368-2374. [DOI:10.1093/humrep/deg473]
15. A Rajasekaran RJ. Male Infertility - current trends in the management of azoospermia and oligoasthenoteratozoospermia In: Rao kA. The Infertility Manual. 3rd Ed. New Dehli: Jitendar P Vij; 2009; pp. 179 - 186.
16. Depa-Martynow M, Jedrzejczak P, Pawelczyk L. Pronuclear scoring as a predictor of embryo quality in in vitro fertilization program. Folia Histochem Cytobiol 2007; 45 (Suppl 1): S85-89.
17. Takahashi C, Fujito A, Kazuka M, Sugiyama R, Ito H, Isaka K. Anti-Mullerian hormone substance from follicular fluid is positively associated with success in oocyte fertilization during in vitro fertilization. Fertil Steril 2008; 89: 586-591. [DOI:10.1016/j.fertnstert.2007.03.080]
18. Mashiach R, Amit A, Hasson J, Amzalzg S, Almog B, Ben-Yosef D, et al. Follicular fluid levels of anti-Mullerian hormone as a predictor of oocyte maturation, fertilization rate, and embryonic development in patients with polycystic ovary syndrome. Fertil Steril 2010; 93: 2299-2302. [DOI:10.1016/j.fertnstert.2009.01.125]
19. Jancar N, Virant-Klun I, Bokal EV. Serum and follicular endocrine profile is different in modified natural cycles than in cycles stimulated with gonadotropin and gonadotropin-releasing hormone antagonist. Fertil Steril 2009; 92: 2069-2071. [DOI:10.1016/j.fertnstert.2009.06.054]
20. Fanchin R, Mendez Lozano DH, Frydman N, Gougeon A, di Clemente N, Frydman R, et al. Anti-Mullerian hormone concentrations in the follicular fluid of the preovulatory follicle are predictive of the implantation potential of the ensuing embryo obtained by in vitro fertilization. J Clin Endocrinol Metab 2007; 92: 1796-1802. [DOI:10.1210/jc.2006-1053]
21. Wunder DM, Guibourdenche J, Birkhauser MH, Bersinger NA. Anti-Mullerian hormone and inhibin B as predictors of pregnancy after treatment by in vitro fertilization/intracytoplasmic sperm injection. Fertil Steril 2008; 90: 2203-2210. [DOI:10.1016/j.fertnstert.2007.10.078]
22. Cupisti S, Dittrich R, Mueller A, Strick R, Stiegler E, Binder H, et al. Correlations between anti-mullerian hormone, inhibin B, and activin A in follicular fluid in IVF/ICSI patients for assessing the maturation and developmental potential of oocytes. Eur J Med Res 2007; 12: 604-608.
23. Lee JR, Kim SH, Kim SM, Jee BC, Ku SY, Suh CS, et al. Follicular fluid anti-Mullerian hormone and inhibin B concentrations: comparison between gonadotropin-releasing hormone (GnRH) agonist and GnRH antagonist cycles. Fertil Steril 2008; 89: 860-867. [DOI:10.1016/j.fertnstert.2007.04.010]
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