International Journal of
Reproductive Biomedicine
Mon, Nov 25, 2024
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Volume 21, Issue 2 (February 2023)
IJRM 2023, 21(2): 175-184 |
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Khoshgoftar Some Saraii Z, Dianaty S, Rouhollah F, Zare N, Ghorbani Yekta B. Reproductive status of male rat offspring following exposure to methamphetamine during intrauterine life: An experimental study. IJRM 2023; 21 (2) :175-184
URL: http://ijrm.ir/article-1-2322-en.html
URL: http://ijrm.ir/article-1-2322-en.html
Zahra Khoshgoftar Some Saraii1 
, Soroush Dianaty2 , Fatemeh Rouhollah1 , Nayereh Zare3 , Batool Ghorbani Yekta * 4
, Soroush Dianaty2 , Fatemeh Rouhollah1 , Nayereh Zare3 , Batool Ghorbani Yekta * 4
1- Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran University of Medical Sciences, Islamic Azad University, Tehran, Iran.
2- Student Research Committee, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Universal Scientific Education and Research Network (USERN), Tehran, Iran.
3- Department of Anatomical Sciences and Cognitive Neuroscience, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
4- Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. ,bahareh59gh@yahoo.com
2- Student Research Committee, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Universal Scientific Education and Research Network (USERN), Tehran, Iran.
3- Department of Anatomical Sciences and Cognitive Neuroscience, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
4- Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. ,
Abstract: (887 Views)
Background: Methamphetamine abuse during pregnancy is associated with maternal and fetal adverse outcomes. Methamphetamine induces reproductive damage in adults; however, its effect has not been studied during pregnancy.
Objective: To investigate the effects of methamphetamine exposure during pregnancy on the reproductive system.
Materials and Methods: 15 pregnant Wistar rats were divided into 3 groups (n = 5/group), and received daily intraperitoneal injections of saline or methamphetamine (5, and 10 mg/kg) from day 10 until the end of pregnancy. One adult male offspring was selected from each dam. Subjects were euthanized, and their testis was removed. Sperm samples from cauda epididymis were analyzed for sperm concentration, morphology, and motility. Terminal deoxynucleotidyl transferase dUTP nick-end labeling assay was used to detect apoptotic cells. Levels of B-cell lymphoma 2 protein (Bcl-2) and Bcl-2 associated X-protein were measured using Western blot.
Results: Methamphetamine significantly decreased sperm concentration (5 mg vs. saline: p = 0.001, 10 mg vs. saline: p < 0.001), normal sperm morphology (saline vs. 10 mg: p = 0.001), and motility (p: saline vs. 5 mg = 0.004, 5 mg vs. 10 mg = 0.011, saline vs. 10 mg < 0.001) in a dose-dependent manner. There was a significantly higher number of terminal deoxynucleotidyl transferase dUTP nick-end labeling -positive cells and higher exposure. Moreover, Bcl-2 associated X-protein was increased, and Bcl-2 was decreased in these rats.
Conclusion: The present study shows that chronic methamphetamine exposure during intrauterine period can induce apoptosis of seminiferous tubules and decrease sperm quality in adult rats. Moreover, we showed that the intrinsic apoptotic pathway is involved in this process. Further studies are required to identify the complete molecular pathway of these results.
Objective: To investigate the effects of methamphetamine exposure during pregnancy on the reproductive system.
Materials and Methods: 15 pregnant Wistar rats were divided into 3 groups (n = 5/group), and received daily intraperitoneal injections of saline or methamphetamine (5, and 10 mg/kg) from day 10 until the end of pregnancy. One adult male offspring was selected from each dam. Subjects were euthanized, and their testis was removed. Sperm samples from cauda epididymis were analyzed for sperm concentration, morphology, and motility. Terminal deoxynucleotidyl transferase dUTP nick-end labeling assay was used to detect apoptotic cells. Levels of B-cell lymphoma 2 protein (Bcl-2) and Bcl-2 associated X-protein were measured using Western blot.
Results: Methamphetamine significantly decreased sperm concentration (5 mg vs. saline: p = 0.001, 10 mg vs. saline: p < 0.001), normal sperm morphology (saline vs. 10 mg: p = 0.001), and motility (p: saline vs. 5 mg = 0.004, 5 mg vs. 10 mg = 0.011, saline vs. 10 mg < 0.001) in a dose-dependent manner. There was a significantly higher number of terminal deoxynucleotidyl transferase dUTP nick-end labeling -positive cells and higher exposure. Moreover, Bcl-2 associated X-protein was increased, and Bcl-2 was decreased in these rats.
Conclusion: The present study shows that chronic methamphetamine exposure during intrauterine period can induce apoptosis of seminiferous tubules and decrease sperm quality in adult rats. Moreover, we showed that the intrinsic apoptotic pathway is involved in this process. Further studies are required to identify the complete molecular pathway of these results.
Type of Study: Original Article |
Subject:
Fertility & Infertility
References
1. Krasnova IN, Cadet JL. Methamphetamine toxicity and messengers of death. Brain Res Rev 2009; 60: 379-407. [DOI:10.1016/j.brainresrev.2009.03.002] [PMID] [PMCID]
2. Oei JL, Kingsbury A, Dhawan A, Burns L, Feller JM, Clews S, et al. Amphetamines, the pregnant woman and her children: A review. J Perinatol 2012; 32: 737-747. [DOI:10.1038/jp.2012.59] [PMID]
3. Frohmader KS, Bateman KL, Lehman MN, Coolen LM. Effects of methamphetamine on sexual performance and compulsive sex behavior in male rats. Psychopharmacology (Berl) 2010; 212: 93-104. [DOI:10.1007/s00213-010-1930-8] [PMID]
4. Mirjalili T, Kalantar SM, Shams Lahijani M, Sheikhha MH, Talebi A. Congenital abnormality effect of methamphetamine on histological, cellular and chromosomal defects in fetal mice. Iran J Reprod Med 2013; 11: 39-46.
5. Khoradmehr A, Danafar A, Halvaei I, Golzadeh J, Hosseini M, Mirjalili T, et al. Effect of prenatal methamphetamine administration during gestational days on mice. Iran J Reprod Med 2015; 13: 41-48.
6. Šlamberová R. Review of long-term consequences of maternal methamphetamine exposure. Physiol Res 2019; 68 (Suppl.): S219-S231. [DOI:10.33549/physiolres.934360] [PMID]
7. Sithisarn T, Granger DT, Bada HS. Consequences of prenatal substance use. Int J Adolesc Med Health 2012; 24: 105-112. [DOI:10.1515/ijamh.2012.016] [PMID]
8. Yamamoto Y, Yamamoto K, Hayase T, Abiru H, Shiota K, Mori C. Methamphetamine induces apoptosis in seminiferous tubules in male mice testis. Toxicol Appl Pharmacol 2002; 178: 155-160. [DOI:10.1006/taap.2001.9330] [PMID]
9. Alavi SH, Taghavi MM, Moallem SA. Evaluation of effects of methamphetamine repeated dosing on proliferation and apoptosis of rat germ cells. Syst Biol Reprod Med 2008; 54: 85-91. [DOI:10.1080/19396360801952078] [PMID]
10. Nudmamud-Thanoi S, Thanoi S. Methamphetamine induces abnormal sperm morphology, low sperm concentration and apoptosis in the testis of male rats. Andrologia 2011; 43: 278-282. [DOI:10.1111/j.1439-0272.2010.01071.x] [PMID]
11. Nudmamud-Thanoi S, Sueudom W, Tangsrisakda N, Thanoi S. Changes of sperm quality and hormone receptors in the rat testis after exposure to methamphetamine. Drug Chem Toxicol 2016; 39: 432-438. [DOI:10.3109/01480545.2016.1141421] [PMID]
12. Lin JF, Lin YH, Liao PC, Lin YC, Tsai TF, Chou KY, et al. Induction of testicular damage by daily methamphetamine administration in rats. Chin J Physiol 2014; 57: 19-30. [DOI:10.4077/CJP.2014.BAB155] [PMID]
13. Sabour M, Khoradmehr A, Kalantar SM, Danafar AH, Omidi M, Halvaei I, et al. Administration of high dose of methamphetamine has detrimental effects on sperm parameters and DNA integrity in mice. Int J Reprod BioMed 2017; 15: 161-168. [DOI:10.29252/ijrm.15.3.161] [PMID] [PMCID]
14. Singh R, Letai A, Sarosiek K. Regulation of apoptosis in health and disease: The balancing act of BCL-2 family proteins. Nat Rev Mol Cell Biol 2019; 20: 175-193. [DOI:10.1038/s41580-018-0089-8] [PMID] [PMCID]
15. Galluzzi L, Vitale I, Aaronson SA, Abrams JM, Adam D, Agostinis P, et al. Molecular mechanisms of cell death: Recommendations of the nomenclature committee on cell death 2018. Cell Death Differ 2018; 25: 486-541. [DOI:10.1038/s41418-017-0012-4] [PMID] [PMCID]
16. Sengupta P. The laboratory rat: Relating its age with human's. Int J Prev Med 2013; 4: 624-630.
17. Campion SN, Carvallo FR, Chapin RE, Nowland WS, Beauchamp D, Jamon R, et al. Comparative assessment of the timing of sexual maturation in male Wistar Han and Sprague-Dawley rats. Reprod Toxicol 2013; 38: 16-24. [DOI:10.1016/j.reprotox.2013.02.003] [PMID]
18. Krzanowska H. The passage of abnormal spermatozoa through the uterotubal junction of the mouse. J Reprod Fertil 1974; 38: 81-90. [DOI:10.1530/jrf.0.0380081] [PMID]
19. Sulzer D, Sonders MS, Poulsen NW, Galli A. Mechanisms of neurotransmitter release by amphetamines: A review. Prog Neurobiol 2005; 75: 406-433. [DOI:10.1016/j.pneurobio.2005.04.003] [PMID]
20. Ramamoorthy JD, Ramamoorthy S, Leibach FH, Ganapathy V. Human placental monoamine transporters as targets for amphetamines. Am J Obstet Gynecol 1995; 173: 1782-1787. [DOI:10.1016/0002-9378(95)90427-1] [PMID]
21. Barenys M, Gomez-Catalan J, Camps L, Teixido E, de Lapuente J, Gonzalez-Linares J, et al. MDMA (ecstasy) delays pubertal development and alters sperm quality after developmental exposure in the rat. Toxicol Lett 2010; 197: 135-142. [DOI:10.1016/j.toxlet.2010.05.009] [PMID]
22. Fazelipour S, Jahromy MH, Tootian Z, Kiaei SB, Sheibani MT, Talaee N. The effect of chronic administration of methylphenidate on morphometric parameters of testes and fertility in male mice. J Reprod Infertil 2012; 13: 232-236.
23. Płotka J, Narkowicz S, Polkowska Ż, Biziuk M, Namieśnik J. Effects of addictive substances during pregnancy and infancy and their analysis in biological materials. In: Whitacre DM. Reviews of environmental contamination and toxicology, Volume 227. Cham: Springer International Publishing; 2014. [DOI:10.1007/978-3-319-01327-5_2] [PMID]
24. Wells PG, McCallum GP, Chen CS, Henderson JT, Lee CJJ, Perstin J, et al. Oxidative stress in developmental origins of disease: Teratogenesis, neurodevelopmental deficits, and cancer. Toxicol Sci 2009; 108: 4-18. [DOI:10.1093/toxsci/kfn263] [PMID]
25. Joya X, Pujadas M, Falcón M, Civit E, Garcia-Algar O, Vall O, et al. Gas chromatography-mass spectrometry assay for the simultaneous quantification of drugs of abuse in human placenta at 12th week of gestation. Forensic Sci Int 2010; 196: 38-42. [DOI:10.1016/j.forsciint.2009.12.044] [PMID]
26. Ganapathy V. Drugs of abuse and human placenta. Life Sciences 2011; 88: 926-930. [DOI:10.1016/j.lfs.2010.09.015] [PMID] [PMCID]
27. Cordeaux Y, Missfelder-Lobos H, Charnock-Jones DS, Smith GCS. Stimulation of contractions in human myometrium by serotonin is unmasked by smooth muscle relaxants. Reprod Sci 2008; 15: 727-734. [DOI:10.1177/1933719108317582] [PMID]
28. Zheng T, Liu L, Shi J, Yu X, Xiao W, Sun R, et al. The metabolic impact of methamphetamine on the systemic metabolism of rats and potential markers of methamphetamine abuse. Mol Biosyst 2014; 10: 1968-1977. [DOI:10.1039/C4MB00158C] [PMID]
29. Slamberová R, Charousová P, Pometlová M. Methamphetamine administration during gestation impairs maternal behavior. Dev Psychobiol 2005; 46: 57-65. [DOI:10.1002/dev.20042] [PMID]
30. Matsumoto RR, Seminerio MJ, Turner RC, Robson MJ, Nguyen L, Miller DB, et al. Methamphetamine-induced toxicity: An updated review on issues related to hyperthermia. Pharmacol Ther 2014; 144: 28-40. [DOI:10.1016/j.pharmthera.2014.05.001] [PMID] [PMCID]
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