Volume 20, Issue 12 (December 2022)
IJRM 2022, 20(12): 1007-1012 |
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Zhima Akhavansales * 
1, Alimohammad Mosadegh Mehrjardi2 , Hamid reza Ashrafzadeh3 , Shadnaz fakhteh Yavari4 , Mohammad Taher Tahoori5 , Morteza Bitaraf Sani6 , Mahnaz Mohammadi3 , Fateme Montazeri3 , Nasrin Ghasemi3
1, Alimohammad Mosadegh Mehrjardi2 , Hamid reza Ashrafzadeh3 , Shadnaz fakhteh Yavari4 , Mohammad Taher Tahoori5 , Morteza Bitaraf Sani6 , Mahnaz Mohammadi3 , Fateme Montazeri3 , Nasrin Ghasemi3
1- Department of Immunology, Faculty of Medicine, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. , dr.zhima@live.com
2- Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.
5- Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Animal Science Research Department, Yazd Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education & Extension Organization (AREEO), Yazd, Iran.
2- Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.
5- Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Animal Science Research Department, Yazd Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education & Extension Organization (AREEO), Yazd, Iran.
Abstract: (609 Views)
Abstract
Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as FAS and FASL genes, which play a fundamental role in embryogenesis and cellular homeostasis.
Objective: The study aimed to investigate the potential role of FAS and FASL genes in POF pathogenesis.
Materials and Methods: In this case-control study, the polymorphisms of FAS-670A/G and FASLIVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method.
Results: Our results revealed that homozygous FAS-670A/A and G/G, and heterozygous FAS-670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of FASIVS2nt_124, polymorphisms were not related to POF risk (p = 0.23).
Conclusion: There is no statistical association between these polymorphisms and POF risk in women referred to genetic counseling clinics.
Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as FAS and FASL genes, which play a fundamental role in embryogenesis and cellular homeostasis.
Objective: The study aimed to investigate the potential role of FAS and FASL genes in POF pathogenesis.
Materials and Methods: In this case-control study, the polymorphisms of FAS-670A/G and FASLIVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method.
Results: Our results revealed that homozygous FAS-670A/A and G/G, and heterozygous FAS-670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of FASIVS2nt_124, polymorphisms were not related to POF risk (p = 0.23).
Conclusion: There is no statistical association between these polymorphisms and POF risk in women referred to genetic counseling clinics.
Type of Study: Original Article |
Subject:
Reproductive Genetics
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