Volume 22, Issue 3 (March 2024)                   IJRM 2024, 22(3): 177-190 | Back to browse issues page

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Karami N, Iravani F, Bakhshandeh Bavarsad S, Asadollahi S, Hoseini S M, Montazeri F et al . Comparing the advantages, disadvantages and diagnostic power of different non-invasive pre-implantation genetic testing: A literature review. IJRM 2024; 22 (3) :177-190
URL: http://ijrm.ir/article-1-3205-en.html
Comparing the advantages, disadvantages and diagnostic power of different non-invasive pre-implantation genetic testing: A literature review
Noorodin Karami1 , Farzaneh Iravani1 , Sareh Bakhshandeh Bavarsad1 , Samira Asadollahi2 , Seyed Mehdi Hoseini3 , Fateme Montazeri * 4, Seyed Mehdi Kalantar5
1- Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Biotechnology Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Science, Yazd, Iran.
4- Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. , f_montazeri@outlook.com
5- Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract:   (467 Views)
To improve embryo transfer success and increase the chances of live birth in assisted reproductive methods, there is a growing demand for the use of pre-implantation genetic testing (PGT). However, the invasive approaches used in PGT have led to in vitro fertilization failure and abortions, increasing anxiety levels for parents. To address this, non-invasive PGT methods have been introduced, such as the detection of DNA in blastocoel fluid of blastocysts and spent culture media (SCM). These methods have proven to be minimally invasive and effective in detecting aneuploidy in the chromosomes of human embryos. This review aims to explore the different approaches to pre-implantation diagnosis, including invasive and non-invasive methods, with a particular focus on non-invasive PGT (niPGT). The search strategy involved gathering data from scientific databases such as PubMed, Google Scholar, and Science Direct using relevant keywords. The search was conducted until January 2023. In total, 22 studies have successfully reported the detection and amplification of cell-free DNA in the embryonic SCM. It is important to note that niPGT has some limitations, which include differences in indicators such as cell-free DNA amplification rate, concordance, level of maternal DNA contamination, sensitivity, and specificity between SCM samples and biopsied cells. Therefore, more extensive and detailed research is needed to fully understand niPGT's potential for clinical applications.

Keywords: Spent culture media, Non-invasive pre-implantation genetic testing, Biopsy methods, Cell-free embryonic DNA.
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Type of Study: Review Article | Subject: Assisted Reproductive Technologies
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