Volume 22, Issue 12 (December 2024)                   IJRM 2024, 22(12): 1015-1024 | Back to browse issues page

Ethics code: IR.KAUMS.REC.1394.58

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Zamani-Badi T, Karimian M, Amini Mahabadi J, Rafatmanesh A, Nikzad H. IL-6-174 G/C transversion might decrease male infertility risk: A case-control study. IJRM 2024; 22 (12) :1015-1024
URL: http://ijrm.ir/article-1-3217-en.html
IL-6-174 G/C transversion might decrease male infertility risk: A case-control study
Tayyebeh Zamani-Badi1 , Mohammad Karimian2 , Javad Amini Mahabadi3 , Atieh Rafatmanesh4 , Hossein Nikzad * 5
1- Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.
2- Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
3- Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran. & Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
4- Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
5- Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran. , hnikzad1343@gmail.com
Abstract:   (208 Views)
Background: Genetic predispositions have been identified as important factors in male infertility. Among the many genes related to male reproductive function, interleukin 6 (IL-6) has emerged as a key player. Despite the growing recognition of genetic factors in male infertility, the specific association between the IL-6-174 G/C genetic polymorphism and male infertility remains an area that needs further investigation.
Objective: This investigation explores the correlation between the IL-6-174 G/C transversion and male infertility.
Materials and Methods: In a case-control study, a total of 314 men who referred to the Kashan Infertility Centre, Shahid Beheshti hospital, Kashan, Iran, were enrolled for IL-6-174 G/C polymorphism analysis. The study comprised 163 infertile participants as the case group and 151 fertile men as the control group. Following the screening, 2 ml of whole blood was collected from each participant. Cases were categorized into 3 subgroups based on World Health Organization criteria: (i) nonobstructive azoospermia (n = 42), (ii) oligozoospermia (n = 61), and (iii) asthenozoospermia (n = 60). After DNA extraction, genotypes of the samples at the -174 G/C (rs1800795) locus were determined using the polymerase chain reaction-restriction fragment length polymorphism method.
Results: Our genetic investigation demonstrated a significant association between the GC genotype and male infertility. Furthermore, a correlation was observed between the heterozygous GC genotype and reduced risk of oligozoospermia and asthenospermia. Additionally, the C allele was correlated with a decreased risk of infertility and specific subgroups such as oligozoospermia and asthenospermia.
Conclusion: Our findings suggest that the IL-6-174 G/C transversion could potentially serve as a protective genetic factor against male infertility.
Keywords: Polymorphism, Genetic, Interleukin-6, Spermatogenesis, Male infertility.
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Type of Study: Original Article | Subject: Reproductive Genetics
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