International Journal of
Reproductive Biomedicine
Thu, Mar 13, 2025
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Volume 23, Issue 1 (January 2025)
IJRM 2025, 23(1): 91-102 |
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Ethics code: IR.SSU.AEC.1401.015
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Nabi A, Hassanpour Dehnavi A, Mazaheri F, Momeni N, Nikukar H, Hemati M, et al . Radioprotective effect of omeprazole against testicular damage induced by ionizing radiation in mice: An experimental study. IJRM 2025; 23 (1) :91-102
URL: http://ijrm.ir/article-1-3421-en.html
URL: http://ijrm.ir/article-1-3421-en.html
Ali Nabi1 
, Azam Hassanpour Dehnavi2 , Fahime Mazaheri3 , Nastaran Momeni4 , Habib Nikukar5 , Mahdie Hemati6 , Seyed Jalal Hosseinimehr7 , Masoud Shabani8 , Fatemeh Sadeghian-Nodoushan9 , Javad Biabani-Ardakani * 10
, Azam Hassanpour Dehnavi2 , Fahime Mazaheri3 , Nastaran Momeni4 , Habib Nikukar5 , Mahdie Hemati6 , Seyed Jalal Hosseinimehr7 , Masoud Shabani8 , Fatemeh Sadeghian-Nodoushan9 , Javad Biabani-Ardakani * 10
1- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Anatomical Sciences and Cell Biology, Medical Faculty, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Shahid Ramezanzadeh Radiation Therapy Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & School of Advanced Medical Sciences and Technologies, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
7- Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
8- Department of Radiation Oncology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
9- Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
10- 1. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2. Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 3. School of Advanced Medical Sciences and Technologies, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,javadbiabani@ssu.ac.ir
2- Department of Anatomical Sciences and Cell Biology, Medical Faculty, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Shahid Ramezanzadeh Radiation Therapy Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. & School of Advanced Medical Sciences and Technologies, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
7- Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
8- Department of Radiation Oncology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
9- Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
10- 1. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2. Biotechnology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 3. School of Advanced Medical Sciences and Technologies, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (59 Views)
Background: Radiation-induced normal tissue damage remains a major concern in radiotherapy, particularly affecting rapidly dividing cells, including those in reproductive tissues. Developing effective radioprotective agents to mitigate this damage is crucial for preserving fertility.
Objective: The radioprotective effects of omeprazole (OMP) were investigated in adult male mice undergoing external radiation therapy.
Materials and Methods: In this experimental study, 36 adult male mice (30-35 gr, 6-8 wk old) were divided into 6 groups and orally administered OMP daily via oral gavage for 7 days before whole-body irradiation. On the 8th day, mice were subjected to a single 6 Gray (Gy) dose of 6 megavoltage X-ray radiation. Blood samples were collected via cardiac puncture for testosterone level evaluation, while testicular specimens were harvested post-euthanasia for sperm parameters assessment and histological analysis. Additionally, spermatogenic cell density was evaluated.
Results: Irradiation of 6 Gray X-ray to the testis of mice significantly affected sperm count, progressive motility, DNA fragmentation, the number of sperm with normal morphology, and the number of immotile sperm. Furthermore, administration of OMP improved progressive motility, DNA fragmentation, and sperm viability. Histopathological findings showed irradiation led to severe testicular atrophy with spermatogenic arrest and abnormal cytoarchitecture vacuolation and interstitial edema, while OMP treatment reversed relative radiation toxicity, especially in the 50 mg OMP treatment group.
Conclusion: In conclusion, OMP could act as an effective radioprotector against testicular damage following X-ray irradiation in an animal model. Further studies are needed to investigate OMP potential in protecting human testis tissue.
Objective: The radioprotective effects of omeprazole (OMP) were investigated in adult male mice undergoing external radiation therapy.
Materials and Methods: In this experimental study, 36 adult male mice (30-35 gr, 6-8 wk old) were divided into 6 groups and orally administered OMP daily via oral gavage for 7 days before whole-body irradiation. On the 8th day, mice were subjected to a single 6 Gray (Gy) dose of 6 megavoltage X-ray radiation. Blood samples were collected via cardiac puncture for testosterone level evaluation, while testicular specimens were harvested post-euthanasia for sperm parameters assessment and histological analysis. Additionally, spermatogenic cell density was evaluated.
Results: Irradiation of 6 Gray X-ray to the testis of mice significantly affected sperm count, progressive motility, DNA fragmentation, the number of sperm with normal morphology, and the number of immotile sperm. Furthermore, administration of OMP improved progressive motility, DNA fragmentation, and sperm viability. Histopathological findings showed irradiation led to severe testicular atrophy with spermatogenic arrest and abnormal cytoarchitecture vacuolation and interstitial edema, while OMP treatment reversed relative radiation toxicity, especially in the 50 mg OMP treatment group.
Conclusion: In conclusion, OMP could act as an effective radioprotector against testicular damage following X-ray irradiation in an animal model. Further studies are needed to investigate OMP potential in protecting human testis tissue.
Type of Study: Original Article |
Subject:
Reproductive Andrology
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