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Reproductive Biomedicine
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Volume 11, Issue 4 (6-2013)
IJRM 2013, 11(4): 267-0 |
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Morán-Martínez J, Carranza-Rosales P, Morales-Vallarta M, Heredia-Rojas J A, Bassol-Mayagoitia S, Betancourt-Martínez N D et al . Chronic environmental exposure to lead affects semen quality in a Mexican men population. IJRM 2013; 11 (4) :267-0
URL: http://ijrm.ir/article-1-412-en.html
URL: http://ijrm.ir/article-1-412-en.html
Javier Morán-Martínez * 
1, Pilar Carranza-Rosales2 , Mario Morales-Vallarta3 , José A. Heredia-Rojas3 , Susana Bassol-Mayagoitia4 , Nadia Denys Betancourt-Martínez5 , Ricardo M. Cerda-Flores6
1, Pilar Carranza-Rosales2 , Mario Morales-Vallarta3 , José A. Heredia-Rojas3 , Susana Bassol-Mayagoitia4 , Nadia Denys Betancourt-Martínez5 , Ricardo M. Cerda-Flores6
1- Departamento de Biología Celular y Ultraestructura, Centro de Investigación Biomédica Facultad de Medicina, Universidad Autónoma de Coahuila, Coahuila, México , javmoran@yahoo.com
2- Departamento de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, México
3- Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León
4- Departamento de Biología de la Reproducción, Centro de Investigación Biomédica Facultad de Medicina, Universidad Autónoma de Coahuila, Coahuila, México
5- Departamento de Biología Celular y Ultraestructura, Centro de Investigación Biomédica Facultad de Medicina, Universidad Autónoma de Coahuila, Coahuila, México
6- Unidad de Biología Molecular, Genómica y Secuenciación. Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, México
2- Departamento de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, México
3- Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León
4- Departamento de Biología de la Reproducción, Centro de Investigación Biomédica Facultad de Medicina, Universidad Autónoma de Coahuila, Coahuila, México
5- Departamento de Biología Celular y Ultraestructura, Centro de Investigación Biomédica Facultad de Medicina, Universidad Autónoma de Coahuila, Coahuila, México
6- Unidad de Biología Molecular, Genómica y Secuenciación. Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, México
Abstract: (2638 Views)
Background: Male infertility is affected by several factors. Lead is one of the heavy metals more bioavailable than usually modifies the sperm quality in humans.
Objective: The aim of this study was to establish the role of lead in semen quality in environmentally exposed men.
Materials and Methods: Semen and blood samples were obtained from two groups: the exposed group (EG=20) and the non-exposed group (NEG=27). Two semen aliquots were used, one to evaluate spermatic quality and the other for lead determination. Blood (PbB) and semen lead (PbS) determination was performed by atomic absorption spectrophotometry.
Results: The PbB concentration was significantly greater in the EG, 10.10±0.97 μgdL-1 than in the NEG, 6.42±0.38 μgdL-1 (p<0.01), as well as the PbS concentration, with 3.28±0.35 and 1.76±0.14μgdL-1 in the EG and NEG respectively (p=0.043). A significant correlation between PbS and PbB concentration in the EG was found (r=0.573, p=0.038). Overall, the spermatic quality was lower in the EG than in the NEG. Specifically, there were significant differences in the spermatic concentration [EG=43.98±6.26 and NEG=68.78±8.51X106 cellmL-1 (p<0.01)], motility [EG=49±7 and NEG=67±4% (p=0.029)], viability [EG=36.32±3.59 and NEG=72.12±1.91% (p<0.01)] and abnormal morphology [EG=67±18 and NEG=32±12% (p<0.01)]. In the immature germ cells (IGC) concentration differences were found only for A cells (EG=8.1±1.1x100 and NEG=3.2±1.9X100 spermatozoa) (p<0.01) and for Sab cells (EG=3.4±2.2x100 and NEG=1.1±1.0X100 spermatozoa) (p=0.041).
Conclusion: These results suggest that chronic environmental exposure to low levels of lead adversely affect the spermatic quality.
Objective: The aim of this study was to establish the role of lead in semen quality in environmentally exposed men.
Materials and Methods: Semen and blood samples were obtained from two groups: the exposed group (EG=20) and the non-exposed group (NEG=27). Two semen aliquots were used, one to evaluate spermatic quality and the other for lead determination. Blood (PbB) and semen lead (PbS) determination was performed by atomic absorption spectrophotometry.
Results: The PbB concentration was significantly greater in the EG, 10.10±0.97 μgdL-1 than in the NEG, 6.42±0.38 μgdL-1 (p<0.01), as well as the PbS concentration, with 3.28±0.35 and 1.76±0.14μgdL-1 in the EG and NEG respectively (p=0.043). A significant correlation between PbS and PbB concentration in the EG was found (r=0.573, p=0.038). Overall, the spermatic quality was lower in the EG than in the NEG. Specifically, there were significant differences in the spermatic concentration [EG=43.98±6.26 and NEG=68.78±8.51X106 cellmL-1 (p<0.01)], motility [EG=49±7 and NEG=67±4% (p=0.029)], viability [EG=36.32±3.59 and NEG=72.12±1.91% (p<0.01)] and abnormal morphology [EG=67±18 and NEG=32±12% (p<0.01)]. In the immature germ cells (IGC) concentration differences were found only for A cells (EG=8.1±1.1x100 and NEG=3.2±1.9X100 spermatozoa) (p<0.01) and for Sab cells (EG=3.4±2.2x100 and NEG=1.1±1.0X100 spermatozoa) (p=0.041).
Conclusion: These results suggest that chronic environmental exposure to low levels of lead adversely affect the spermatic quality.
Type of Study: Original Article |
References
1. Assennato G, Paci C, Baser ME, Molinini R, Candela RG, Altamura BM, et al. Sperm count suppression without endocrine dysfunction in lead-exposed men. Arch Environ Health 1987; 2: 124-127. [DOI:10.1080/00039896.1987.9935808]
2. Lancrajan I, Popescu HI, Gavanescu O, Klepsch I, Serbanescu M. Reproductive ability of workmen occupationally exposed to lead. Arch Environ Health 1975; 30: 396-401. [DOI:10.1080/00039896.1975.10666733]
3. Lerda D. Study of sperm characteristics in persons occupationally exposed to lead. Am J Ind Med 1992; 22: 396-401. [DOI:10.1002/ajim.4700220411]
4. Morán-Martínez J. Effects of environmental lead on sperm motility in men. International Conference on Reproductive Health. Bombay, India 1998; 332.
5. Vigeh M, Smith DR, Hsu PC. How does lead induce male infertility? Iran J Reprod Med 2011; 9: 1-8.
6. Jockenhövel F, Bals-Pratsch M, Bertram HP, Nieschlag E. Seminal lead and copper in fertile men Andrologia 1990; 22: 503-511.
7. Molinini R, Assenato G, Galiardi T, Paci C. Lead effects on male fertility in battery workers. Communication to the international Occupational J health Congress, Cairo 1981; 48.
8. McGregor AJ, Mason MJ. Chronic occupational lead exposure and testicular endocrine function Hum Exp Toxicol 1990; 9: 371-376.
9. World Health Organization. Laboratory Manual for the Examination of Human Semen and semen-Cervical Mucus Interaction. Cambridge University Press; 1999: 45.
10. Brown AA, Halls DJ, Taylor A. Atomic spectrophotometry update-clinical and biological materials; Food and beverages. J Anal At Spectrom 1988; 3: 45. [DOI:10.1039/JA988030045R]
11. World Health Organization. Collection and examination of human semen. In: Beslev MA, Eliasson R, Gallegos AJ, Moghissi KS, Paulsen CA, Prass MRN, editors. Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. Singapore: Press Concern 1980; 9-32.
12. STATA version 6. Stata Corporation, College Station, TX, USA.
13. Calderón-Salinas JV, Hernández-Luna C, Valdez-Anaya B, Maldonado-Vega M, Lopez-Miranda. A Evolution of lead toxicity in population of children. Hum Exp Toxicol 1996; 15: 376-382. [DOI:10.1177/096032719601500502]
14. Calderón-Salinas JV, Valdéz-Anaya B, Zú-iga-Charles Ma, Albores-Medina A. Lead exposure in a population of Mexican children. Human Exp Toxicol 1996; 15: 305-311. [DOI:10.1177/096032719601500406]
15. García-Vargas GG, Rubio-Andrare M, Del Razo LM, Borja-Aburto V, Vera-Aguilar E, Cebrian ME. Lead exposure in children in smelter community in Region Lagunera, Mexico. J Toxicol Environ Health 2001; 62: 417-429. [DOI:10.1080/00984100150501150]
16. Saaranen M, Suistomaa U, Kantola M, Saarikoski S, Vanha-Perttula T. Lead, magnesium, selenium and zinc in human seminal fluid: Comparison with semen parameters and fertility. Hum Reprod 1989; 2: 475-479. [DOI:10.1093/oxfordjournals.humrep.a136573]
17. Butrimovitz GP, Sharlip I, Lo R. Extremely low seminal lead concentrations and male fertility. Clinica Chemica Acta 1983; 135: 229-231. [DOI:10.1016/0009-8981(83)90139-0]
18. El-Zohairy EA, Youssef AF, Abul-Nasr SM, Fahmy IM, Salem D, Kahil AK, et al. Reproductive hazards of lead exposure among urban egyptian men. Reprod Toxicol 1996; 10: 145-151. [DOI:10.1016/0890-6238(95)02057-8]
19. Wyrobeck AJ, Gordon LA, Burkhart JG, Francis MW, Kepp RW Jr, Letz G, et al. An evaluation of human sperm as indicators of chemically-induced alterations of spermatogenic function. Mutat Res 1983; 115: 73-148. [DOI:10.1016/0165-1110(83)90015-5]
20. Robins GT, Bornman MS, Ehrlich IR, Cantrell CA, Pienaar E, Vallabh J, et al. Semen Quality of men employed in South Africa lead acid battery plant. Am J Ind Med 1997; 32: 369-376.
https://doi.org/10.1002/(SICI)1097-0274(199710)32:4<369::AID-AJIM8>3.0.CO;2-P [DOI:10.1002/(SICI)1097-0274(199710)32:43.0.CO;2-P]
21. Chia SE, Ong CN, Lee ST, Tsakok FHM. Blood concentration of lead, cadmium, mercury, zinc and copper and human semen parameters. Arch Androl 1992; 29: 177-183. [DOI:10.3109/01485019208987722]
22. Jeyendran Rs, Van der Ven HH, Perez Perez M, Crabo BG, Zaneveld LJD. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70: 219-227. [DOI:10.1530/jrf.0.0700219]
23. Quintanilla-Vega B, Hoover DJ, Bal W, Silbergeld EK, Waalkes MP, Anderson LD. Lead interaction with human protamine (HP2) as a mechanism of male reproductive toxicity. Chem Res Toxicol 2000; 7: 594-600. [DOI:10.1021/tx000017v]
24. Quintanilla-Vega B, Hoover DJ, Bal W, Silbergeld EK, Waalkes MP, Anderson LD. Lead effects on protamine-DNA binding. Am J Ind Med 2000; 3: 324-329.
https://doi.org/10.1002/1097-0274(200009)38:3<324::AID-AJIM12>3.0.CO;2-R [DOI:10.1002/1097-0274(200009)38:33.0.CO;2-R]
25. Hernández-Ochoa I, Sánchez-Gutíerrez M, Solís-Heredia MJ, Quintanilla-Vega B. Spermatozoa Nucleous takes up lead during the Epididymal maturation altering Chromatin Condensation. Reprod Toxicol 2006; 2: 171-178. [DOI:10.1016/j.reprotox.2005.07.015]
26. Benoff S, Cooper GW, Centola GM, Jacob A, Hershlag A, Hurley IR. Metal Ions and Human Sperm Mannose Receptors. Andrologia 2000; 32: 317-329. [DOI:10.1046/j.1439-0272.2000.00401.x]
27. Bassol S, Recio R, De la Cruz DL. Frequency of immature germinal cells in normal and abnormal semen analysis. Arch Androl 1990; 25: 115-119. [DOI:10.3109/01485019008987602]
28. Thomas JA. Toxic responses of the reproductive system In: Casarett and Doull's. Toxicology. The Basic Scienjce of poisons. Curtis D. Klaassen, Mary O. Amdur, John Doull (eds). 5th Ed. McGraw-Hill. USA 1996; 547-581.
29. Bell JU, Thomas JA. Effects of lead on the reproductive system. In Singhal RL, Thomas JA (eds): Basic and clinical toxicity of lead. Baltimore Urban and Schawarzenberg Medical Publishers 1980; 169-189.
30. Noack-Füller G, De Beer C, Seibert H. Cadmium, lead, selenium, and zinc in semen of occupationally exposed men. Andrologia 1993; 25: 7-12. [DOI:10.1111/j.1439-0272.1993.tb02674.x]
31. Diario Oficial de la Federación Diario official de la Federación de México CDXCV (23 de Diciembre) 1994; 4.
32. U.S. Environmental Protection Agency. Engineering Bulletin: Technology Alternatives for the Remediation of Soils Contaminated with As, Cd, Cr, Hg and Pb. EPA/540/S-97/500. Washington, DC: U.S. EPA, Office of Emergency and Remedial Response. 1997.
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