Volume 11, Issue 5 (7-2013)                   IJRM 2013, 11(5): 405-0 | Back to browse issues page

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Cetin C, Serdaroglu H, Tuzlali S. The importance of endometrial nerve fibers and macrophage cell count in the diagnosis of endometriosis. IJRM 2013; 11 (5) :405-0
URL: http://ijrm.ir/article-1-416-en.html
The importance of endometrial nerve fibers and macrophage cell count in the diagnosis of endometriosis
Cihan Cetin * 1, Hasan Serdaroglu2 , Sitki Tuzlali3
1- Department of Obstetrics and Gynecology, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey , cihancetin00@gmail.com
2- Department of Obstetrics and Gynecology, Reproductive Endocrinology and Infertility Unit, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey
3- Department of Pathology, Gynecopathology Unit, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey
Abstract:   (2354 Views)
Background: Endometriosis is a disease that is hard to diagnose without the gold standard method, laparoscopy. An easier diagnostic method is needed.
Objective: The aim of the study is to determine whether the number of macrophage cells in the endometrium and/or the detection of nerve fibers can be used in the diagnosis of endometriosis.
Materials and Methods: Endometrial sampling was done to 31 patients prior to laparoscopy (L/S) or laparotomy (L/T) at Istanbul University Istanbul School of Medicine Hospital between January 2010 February 2011. Also 34 patients who were retrospectively chosen from their files were added to the study. 5 patients were excluded from the study. Totally, 31 patients were placed in the endometriosis and 29 patients in the control group. Endometrial samples were evaluated immunohistochemically with the markers protein gene product 9.5 (PGP 9.5) and neurofilament (NF) for nerve fibers and CD68 for macrophages.
Results: None of the samples were stained with PGP 9.5 and NF. As for CD68+cells, no statistically significant difference was observed between groups (endometriosis: 216.10±104.41; control: 175.93±43.05, p=0.06). Results were also evaluated in the subgroups of menstruel phases and disease stages. Only in the proliferative phase there was a significant increase in the endometriosis group (p=0.03). No significant difference was observed between the stages.
Conclusion: The detection of nerve fibers in the eutopic endometrium with the markers of PGP 9.5 and NF is not found to be helpful in the diagnosis of endometriosis. Macrophage cells may be helpful in the diagnosis only in the proliferative phase.
Keywords: Endometriosis, Macrophage, CD68 Antigen, Neurofilament Proteins
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Type of Study: Original Article |
References
1. Lebovic DI, Mueller MD, Taylor RN. Immunobiology of endometriosis. Fertil Steril 2001; 75: 1-10. [DOI:10.1016/S0015-0282(00)01630-7]
2. Al-Jefout M, Dezarnaulds G, Cooper M, Tokushige N, Luscombe GM, Markham R, et al. Diagnosis of endometriosis by detection of nerve fibres in an endometrial biopsy: a double blind study. Hum Reprod 2009; 24: 3019-3024. [DOI:10.1093/humrep/dep275]
3. Ballard K, Lowton K, Wright J. What's the delay? A qualitative study of women's experiences of reaching a diagnosis of endometriosis. Fertil Steril 200; 86: 1296-1301. [DOI:10.1016/j.fertnstert.2006.04.054]
4. Moore J, Copley S, Morris J, Lindsell D, Golding S, Kennedy S. A systematic review of the accuracy of ultrasound in the diagnosis of endometriosis. Ultrasound Obstet Gynecol 2002; 20: 630-634. [DOI:10.1046/j.1469-0705.2002.00862.x]
5. Kennedy S, Bergqvist A, Chapron C, D'Hooghe T, Dunselman G, Greb R, et al. ESHRE guideline for the diagnosis and treatment of endometriosis. Hum Reprod 2005; 20: 2698-2704. [DOI:10.1093/humrep/dei135]
6. Bondza PK, Maheux R, Akoum A. Insights into endometriosis-associated endometrial dysfunctions: a review. Front Biosci (Elite Ed) 2009; 1: 415-428.
7. Zeller JM, Henig I, Radwanska E, Dmowski WP. Enhancement of human monocyte and peritoneal macrophage chemiluminescence activities in women with endometriosis. Am J Reprod Immunol Microbiol 1987; 13: 78-82. [DOI:10.1111/j.1600-0897.1987.tb00097.x]
8. BraunDP, Muriana A, Gebel H, Rotman C, Rana N, Dmowski WP. Monocyte-mediated enhancement of endometrial cell proliferation in women with endometriosis. Fertil Steril 1994; 61: 78-84. [DOI:10.1016/S0015-0282(16)56456-5]
9. Cao WG, Morin M, Metz C, Maheux R, Akoum A. Stimulation of macrophage migration inhibitory factor expression in endometrial stromal cells by interleukin I, beta involving the nuclear transcription factor NFkBI. Biol Reprod 2005; 73: 565-570. [DOI:10.1095/biolreprod.104.038331]
10. Faweett JW, Keynes RJ. Peripheral nerve regeneration. Annu Rev Neurosci 1990; 13: 43-50. [DOI:10.1146/annurev.ne.13.030190.000355]
11. Gulati AK. Immune Response and Neurotrophic Factor Interactions in Peripheral Nerve Transplants. Acta Haematol 1998; 99: 171-174. [DOI:10.1159/000040832]
12. Tokushige N, Markham R, Russell P, Fraser IS. High density of small nerve fibres in the functional layer of the endometrium in women with endometriosis. Hum Reprod 2006; 21: 782-787. [DOI:10.1093/humrep/dei368]
13. Aghaey Meibody F, Mehdizadeh Kashi A, Zare Mirzaie A, Ghajarie Bani Amam M, Shariati Behbahani A, Zolali B, et al. Diagnosis of endometrial nerve fibers in women with endometriosis. Arch Gynecol Obstet 2011; 284: 1157-1162. [DOI:10.1007/s00404-010-1806-5]
14. Bokor A, Kyama CM, Vercruysse L, Fassbender A, Gevaert O, Vodolazkaia A, et al. sensory nerve fibres in endometrium: a semi-invasive diagnostic test for minimal to mild endometriosis. Hum Reprod 2009; 24: 3025-3032. [DOI:10.1093/humrep/dep283]
15. Zhang X, Lu B, Huang X, Xu H, Zhou C, Lin J. Endometrial nerve fibers in women with endometriosis, adenomyosis, and uterine fibroids. Fertil Steril 2009; 92: 1799-1801. [DOI:10.1016/j.fertnstert.2009.05.016]
16. Schulke L, Berbic M, Manconi F, Tokushige N, Markham R, Fraser IS. Dendritic cell populations in the eutopic and ectopic endometrium of women with endometriosis. Hum Reprod 2009; 24: 1695-1703. [DOI:10.1093/humrep/dep071]
17. Xu W, Roos A, Daha MR, van Kooten C. Dendritic cell and macrophage subsets in the handling of dying cells. Immunobiology 2006; 211: 567-575. [DOI:10.1016/j.imbio.2006.05.023]
18. Braun DP, Ding J, Shen J, Rana N, Fernandez BB, Dmowski WP. Relationship between apoptosis and the number of macrophages in eutopic endometrium from women with and without endometriosis. Fertil Steril 2002; 78: 830-835. [DOI:10.1016/S0015-0282(02)03334-4]
19. Khan KN, Masuzaki H, Fujishita A, Kitajima M, Sekine I, Ishimaru T. Differential macrophage infiltration in early and advanced endometriosis and adjacent peritoneum. Fertil Steril 2004; 81: 652-661. [DOI:10.1016/j.fertnstert.2003.07.037]
20. Berbic M, Schulke L, Markham R, Tokushige N, Russell P, Fraser IS. Macrophage expression in endometrium of women with and without endometriosis. Hum Reprod 2009; 24: 325-332. [DOI:10.1093/humrep/den393]
21. Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol 2004; 25: 677-686. [DOI:10.1016/j.it.2004.09.015]
22. Salamonsen LA, Lathbury LJ. Endometrial leukocytes and menstruation. Hum Reprod Update 2000; 6: 16-27. [DOI:10.1093/humupd/6.1.16]
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