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Reproductive Biomedicine
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Volume 12, Issue 11 (12-2014)
IJRM 2014, 12(11): 755-0 |
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Khanaki K, Sadeghi M R, Akhondi M M, Darabi M, Mehdizadeh A, Shabani M, et al . High ω-3:ω-6 fatty acids ratio increases fatty acid binding protein 4 and extracellular secretory phospholipase A2IIa in human ectopic endometrial cells. IJRM 2014; 12 (11) :755-0
URL: http://ijrm.ir/article-1-495-en.html
URL: http://ijrm.ir/article-1-495-en.html
Korosh Khanaki1 
, Mohammad Reza Sadeghi1 , Mohammad Mehdi Akhondi1 , Masoud Darabi2 , Amir Mehdizadeh3 , Mahdi Shabani4 , Ali Rahimipour5 , Mohammad Nouri * 6
, Mohammad Reza Sadeghi1 , Mohammad Mehdi Akhondi1 , Masoud Darabi2 , Amir Mehdizadeh3 , Mahdi Shabani4 , Ali Rahimipour5 , Mohammad Nouri * 6
1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2- Umbilica Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3- Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4- Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
5- Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran ,nourimd@yahoo.com
2- Umbilica Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3- Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4- Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
5- Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran ,
Abstract: (3157 Views)
Background: Endometriosis, a common chronic inflammatory disorder, is defined by the atypical growth of endometrium- like tissue outside of the uterus. Secretory phospholipase A2 group IIa (sPLA2-IIa) and fatty acid binding protein4 (FABP4) play several important roles in the inflammatory diseases.
Objective: Due to reported potential anti-inflammatory effects of ω-3 and ω-6 fatty acids, the purpose of the present study was to investigate the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) on fatty acid binding protein 4 and extracellular secretory phospholipase A2IIa in cultured endometrial cells.
Materials and Methods: Ectopic and eutopic endometrial tissues obtained from 15 women were snap frozen. After thawing and tissue digestion, primary mixed stromal and endometrial epithelial cell culture was performed for 8 days in culture mediums supplemented with normal and high ratios of ω-3 and ω-6 PUFA. sPLA2-IIa in the culture medium and FABP4 level was determined using enzyme immuno assay (EIA) technique.
Results: Within ectopic endometrial cells group, the level of cellular FABP4 and extracellular sPLA2-IIa were remarkably increased under high ω-3 PUFA exposure compared with control condition (p=0.014 and p=0.04 respectively).
Conclusion: ω-3 PUFAs may increase the level of cellular FABP4 and extracellular sPLA2-IIa in ectopic endometrial cells, since sPLAIIa and FABP4 may affect endometriosis via several mechanisms, more relevant studies are encouraged to know the potential effect of increased cellular FABP4 and extracellular sPLA2-IIa on endometriosis.
Objective: Due to reported potential anti-inflammatory effects of ω-3 and ω-6 fatty acids, the purpose of the present study was to investigate the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) on fatty acid binding protein 4 and extracellular secretory phospholipase A2IIa in cultured endometrial cells.
Materials and Methods: Ectopic and eutopic endometrial tissues obtained from 15 women were snap frozen. After thawing and tissue digestion, primary mixed stromal and endometrial epithelial cell culture was performed for 8 days in culture mediums supplemented with normal and high ratios of ω-3 and ω-6 PUFA. sPLA2-IIa in the culture medium and FABP4 level was determined using enzyme immuno assay (EIA) technique.
Results: Within ectopic endometrial cells group, the level of cellular FABP4 and extracellular sPLA2-IIa were remarkably increased under high ω-3 PUFA exposure compared with control condition (p=0.014 and p=0.04 respectively).
Conclusion: ω-3 PUFAs may increase the level of cellular FABP4 and extracellular sPLA2-IIa in ectopic endometrial cells, since sPLAIIa and FABP4 may affect endometriosis via several mechanisms, more relevant studies are encouraged to know the potential effect of increased cellular FABP4 and extracellular sPLA2-IIa on endometriosis.
Keywords: Endometriosis, Secretory phospholipaseA2IIa, Fatty acid binding protein 4, n-3 polyunsaturated fatty acid, Cell culture
Type of Study: Original Article |
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