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Reproductive Biomedicine
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Volume 12, Issue 4 (5-2014)
IJRM 2014, 12(4): 269-274 |
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Asgharnia M, Mirblouk F, Salamat F, Ashrafkhani B, Dirbaz Z. Predictive value of aspartate aminotransferase and alanine aminotransferase levels in vaginal fluid for the diagnosis of premature rupture of membranes. IJRM 2014; 12 (4) :269-274
URL: http://ijrm.ir/article-1-528-en.html
URL: http://ijrm.ir/article-1-528-en.html
1- Reproductive Health Research Center, Department of Obstetrics and Gynecology, Guilan University of Medical Sciences, Rasht, Iran
2- Reproductive Health Research Center, Department of Obstetrics and Gynecology, Guilan University of Medical Sciences, Rasht, Iran , faribamirblouk@yahoo.com
3- Guilan University of Medical Sciences, Rasht, Iran
2- Reproductive Health Research Center, Department of Obstetrics and Gynecology, Guilan University of Medical Sciences, Rasht, Iran , faribamirblouk@yahoo.com
3- Guilan University of Medical Sciences, Rasht, Iran
Abstract: (2414 Views)
Background: Preterm premature rupture of membranes (PPROM) occurs in 3% of pregnancies and 30-40% of preterm labors are related to this problem. Early diagnosis of PPROM is very important due to its impact on pregnancy outcomes.
Objective: To determine the diagnostic value of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in vaginal fluid for the diagnosis of preterm premature rupture of membranes as a non-invasive and available test.
Materials and Methods: A total of 148 pregnant women between the 26th-36th gestational weeks were enrolled in the study. 74 patients were in PROM group and 74 in control group. AST and ALT levels in vaginal fluid were measured in each group. Mann Whitney U-test was used to compare AST and ALT levels in each group.
Results: The mean of AST level in vaginal fluid was 12.77±10.06 in PROM group vs. 6.91±10.92 in control group (p<0.001), while there were no significant difference between ALT levels in PROM group 1.51±3.17 and control group 0.89±1.15 (p=0.49). Optimal cut point of AST for the diagnosis of PROM was 4.5 IU/L in this study. The sensitivity, specificity, positive and negative predictive values were 82.4%, 63.5%, 69.32% and 78.33% respectively.
Conclusion: According to the findings of this study, measurement of AST level in vaginal fluid can be used as a reliable test for diagnosis of PROM, but there is no good cut point for ALT level that can be practically used.
Objective: To determine the diagnostic value of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in vaginal fluid for the diagnosis of preterm premature rupture of membranes as a non-invasive and available test.
Materials and Methods: A total of 148 pregnant women between the 26th-36th gestational weeks were enrolled in the study. 74 patients were in PROM group and 74 in control group. AST and ALT levels in vaginal fluid were measured in each group. Mann Whitney U-test was used to compare AST and ALT levels in each group.
Results: The mean of AST level in vaginal fluid was 12.77±10.06 in PROM group vs. 6.91±10.92 in control group (p<0.001), while there were no significant difference between ALT levels in PROM group 1.51±3.17 and control group 0.89±1.15 (p=0.49). Optimal cut point of AST for the diagnosis of PROM was 4.5 IU/L in this study. The sensitivity, specificity, positive and negative predictive values were 82.4%, 63.5%, 69.32% and 78.33% respectively.
Conclusion: According to the findings of this study, measurement of AST level in vaginal fluid can be used as a reliable test for diagnosis of PROM, but there is no good cut point for ALT level that can be practically used.
Type of Study: Original Article |
References
1. Movahed F, Yarahmadi Z, Samei Rad F, Khani S. Diagnostic value of lactate dehydrogenase and creatinine in diagnosis of premature rupture of membranes and vaginal fluid. J Mazand Univ Med Sci 2009; 76: 53-42. (In Persian)
2. Kishida T, Yamada H, Negishi H, Sagawa T, Makinoda S, Fujimoto S. Diagnosis of preterm premature rupture of the membranes using a newly developed AFP monoclonal antibody test kit. Eur J Obstet Gynecol Reprod Biol 1995; 58: 67-72. [DOI:10.1016/0028-2243(94)01973-B]
3. Esim E, Turan C, Unal O, Dansuk R, Cengizglu B. Diagnosis of premature rupture of membranes by identification of βHCG in vaginal washing fluid. Eur J Obstet Gynecol Reprod Biol 2003; 107: 37-40. [DOI:10.1016/S0301-2115(02)00277-4]
4. Ladfors L. Prelabour rupture of them embranes at or near term: clinical and epidemiological studies. PhD Thesis, University of Gothenburg, Sweden, 1998.
5. Kafali H, Oksüzler C. Vaginal fluid urea and creatinine in diagnosis of premature rupture of membranes. Arch Gynecol Obstet 2007; 275: 157-160. [DOI:10.1007/s00404-006-0240-1]
6. Kale E, Kuyumcuoğlu U, Kale A, Güzel AI, Canoruc N. A new and practical aspartate aminotransferase test in vaginal washing fluid for the detection of preterm premature rupture of membranes. Fetal Diagn Ther 2008; 24: 425-428. [DOI:10.1159/000170093]
7. Cunningham FG, Leveno KJ, Bloom SL, Hauth JC, Rouse DJ, Spong CY. Williams Obstetrics.Preterm Birth. 23th Ed. New York, MC Graw Hill Medical; 2010: 804-832.
8. Akercan F, Cirpan T, Kazandi M, Cosan Terek M, Mgoyi L, Ozkinay E. The value of the insulin-like growth factor binding protein-1 in the cervical-vaginalsecretion detected by immune-chromatographic dipstick test in the prediction of delivery in women withclinically unconfirmed preterm premature rupture of membranes. Eur J Obstet Gynecol Reprod Biol 2005; 121: 159-163. [DOI:10.1016/j.ejogrb.2004.12.006]
9. Shaarawy M, El-Minawi AM. Prolactin and calcitropic hormones in preterm premature rupture of membranes. Int J Gynaecol Obstet 2004; 84: 200-207. [DOI:10.1016/S0020-7292(03)00334-5]
10. Kuczyńska-Sicińska J, Wójcicka-Jagodzińska J, Romejko E, Smolarczyk R, Siekierski BP, Chodzińska B. Biochemical studies of the amniotic fluid in arterial hypertension in relation to intrauterine growth retardation. I. Parameters of the proteins, lipids, enzymes and renal maturity. Ginekol Pol 1989; 60: 266-270.
11. Smolarczyk R, Wójcicka-Jagodzińska J, Romejko E, Czajkowski K, Piekarski P, Teliga-Czajkowska J, et al. Evaluation of fetal condition in pregnancy complicated by hypertension-biochemical assessment of amniotic fluid. II. Enzymes. Acid-base balance. Ginekol Pol 1996; 67: 603-606.
12. Kale A, Kale E, Akdeniz N, Kuyumcuoğlu U, Canoruc N. Proposal of a diagnostic test for the detection of preterm premature rupture of membranes based on the determination of thyroid hormones in vaginal washing fluid. Fetal Diagn Ther 2007; 22: 330-334. [DOI:10.1159/000103290]
13. Anai T, Tanaka Y, Hirota Y, Miyakawa I. Vaginal fluid hCG levels for detecting premature rupture of membranes. Obstet Gynecol 1997; 89: 261-264. [DOI:10.1016/S0029-7844(96)00448-6]
14. Martinez de Tejada B, Boulvain M, Dumps P, Bischof P, Meisser A, Irion O. Can we improve the diagnosis of rupture of membranes? The value of insulin-like growth factor binding protein-1. BJOG 2006; 113: 1096-1099. [DOI:10.1111/j.1471-0528.2006.01028.x]
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