Volume 12, Issue 5 (6-2014)                   IJRM 2014, 12(5): 335-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Pourentezari M, Talebi A, Abbasi A, Khalili M A, Mangoli E, Anvari M. Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice. IJRM 2014; 12 (5) :335-0
URL: http://ijrm.ir/article-1-541-en.html
Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice
Majid Pourentezari1 , Alireza Talebi2 , Abulghasem Abbasi1 , Mohammad Ali Khalili2 , Esmat Mangoli2 , Morteza Anvari * 3
1- Department of Biology and Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , moanvari@yahoo.com
Abstract:   (2761 Views)
Background: Acrylamide (AA) is an important industrial chemical primarily. AA is also found in carbohydrate-rich foods that are prepared at high temperatures, such as French fries and potato chips. It is demonstrated that AA is a carcinogen and reproductive toxin and has ability to induce sperm damage.
Objective: The aim of this study was to observe the effects of AA on sperm parameters and evaluation of sperm chromatin quality and testosterone hormone in mice.
Materials and Methods: Totally, 16 adult male mice were divided into two groups. Mice of group A fed on basal diet; group B received basal diet and AA (10 mg/kg, water solution) for 35 days. The right cauda epididymis was incised and then placed in Ham’s F10 culture media at 37oC for 15 min. Released spermatozoa were used to analyze count, motility, morphology and viability. To determine the sperm DNA integrity and chromatin condensation, the cytochemical techniques including Aniline blue, Acridine orange and Chromomycin A3 staining were used.
Results: AA-treated mice had poor parameters in comparison with control animals. In sperm chromatin assessments, except TB (p=0.16), significant differences were found in all of the tests between two groups. It was also seen a significant decrease in concentration of blood testosterone in AA-treated animals when compared to controls (p<0.001).
Conclusion: According to our results, AA can affect sperm parameters as well as sperm chromatin condensation and DNA integrity in mice. These abnormalities may be related to the reduction in blood testosterone.
Keywords: Mice, Acrylamide, Sperm, Chromatin, Testosterone
Full-Text [PDF 266 kb]   (666 Downloads) |   |   Full-Text (HTML)  (1006 Views)  
Type of Study: Original Article |
References
1. Friedman M. Chemistry, biochemistry, and safety of acrylamide. A review. J Agric Food Chem 2003; 51: 4504-4526. [DOI:10.1021/jf030204+]
2. Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem 2002; 50: 4998-5006. [DOI:10.1021/jf020302f]
3. World Health Organization. FAO/WHO Consultations on the health implications of acrylamide in food. Summary report of a meeting held in Geneva. Geneva; 2002.
4. LoPachin RM, Balaban CD, Ross JF. Acrylamide axonopathy revisited. Toxicol Appl Pharmacol 2003; 188: 135-153. [DOI:10.1016/S0041-008X(02)00072-8]
5. Ghanayem BI, McDaniel LP, Churchwell MI, Twaddle NC, Snyder R, Fennell TR, et al. Role of CYP2E1 in the epoxidation of acrylamide to glycidamide and formation of DNA and hemoglobin adducts. Toxicol Sci 2005; 88: 311-318. [DOI:10.1093/toxsci/kfi307]
6. Ghanayem BI, Witt KL, El-Hadri L, Hoffler U, Kissling GE, Shelby MD, et al. Comparison of germ cell mutagenicity in male CYP2E1-null and wild-type mice treated with acrylamide: evidence supporting a glycidamide-mediated effect. Biol Reprod 2005; 72: 157-163. [DOI:10.1095/biolreprod.104.033308]
7. Ghanayem BI, Witt KL, Kissling GE, Tice RR, Recio L. Absence of acrylamideinducedgenotoxicity in CYP2E1-null mice: evidence consistent with a glycidamide-mediated effect. Mutat Res 2005; 578: 284-297. [DOI:10.1016/j.mrfmmm.2005.05.004]
8. IARC. Acrylamide. IARC Monogr Eval Carcinog Risks Hum. Institute of Laboratory Animal Resources (1996) Guide for the Care and Use of Laboratory Animals 7th Ed. Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council, Washington DC; 1994: 389-4334.
9. Sumner SC, MacNeela JP, Fennell TR. Characterization and quantitation of urinary metabolites of [1, 2, 3-13C] acrylamide in rats and mice using 13C nuclear magnetic resonance spectroscopy. Chem Res Toxicol 1992; 5: 81-89. [DOI:10.1021/tx00025a014]
10. Manjanatha MG, Aidoo A, Shelton SD, Bishop ME, McDaniel LP, Lyn-Cook LE, et al. Genotoxicity of acrylamide and its metabolite glycidamide administered in drinking water to male and female Big Blue mice. Environ Mol Mutagen 2006; 47: 6-17. [DOI:10.1002/em.20157]
11. Shelby MD, Cain KT, Cornett CV, Generoso WM. Acrylamide: induction of heritable translocation in male mice. Environ Mutagen 1987; 9: 363-368. [DOI:10.1002/em.2860090403]
12. Dobrzyn´ska MM, Gajewski AK. Induction of micronuclei in bone marrow and sperm head abnormalities after combined exposure of mice to low doses of X-rays and acrylamide. Teratogen Carcinogen Mutagen 2000; 20: 133-140. https://doi.org/10.1002/(SICI)1520-6866(2000)20:3<133::AID-TCM4>3.0.CO;2-B [DOI:10.1002/(SICI)1520-6866(2000)20:33.0.CO;2-B]
13. Collins, BW, Howard, DR, Allen JW. Kinetochore-staining of spermatid micronuclei: studies of mice treated with X-radiation or acrylamide. Mutat Res 1992; 281: 287-294. [DOI:10.1016/0165-7992(92)90023-B]
14. Tyl RW, Friedman MA. Effects of acrylamide on rodent reproductive performance. Reprod Toxicol 2003; 17: 1-13. [DOI:10.1016/S0890-6238(02)00078-3]
15. Tyl RW, Marr MC, Myers CB, Ross WP, Friedman M. A. Relationship between acrylamide reproductive and neurotoxicity in male rats. Reprod Toxicol 2000; 14: 147-157. [DOI:10.1016/S0890-6238(00)00066-6]
16. Adler ID, Baumgartner A, Gonda H, Friedman MA, Skerhut M. 1-Aminobenzotriazole inhibits acrylamide-induced dominant lethal effects in spermatids of male mice. Mutagenesis 2000; 15: 133-136. [DOI:10.1093/mutage/15.2.133]
17. Talebi AR. Sperm nuclear maturation. 1thEd. New York, Nova Science Publishers Inc.; 2011: 45-55.
18. Talebi AR, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Firoozabadi RD, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. J Androl 2012; 44: 462-470. [DOI:10.1111/j.1439-0272.2011.01206.x]
19. Talebi AR, Khalili MA, Nahangi H, Abbasi AG, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat. Iran J Reprod Med 2007; 5: 171-176.
20. Carrell DT, Emery BR, Hammoud S. Altered protamine expression and diminished spermatogenesis: What is link? Hum Reprod 2007; 13: 313-327. [DOI:10.1093/humupd/dml057]
21. Kermani-Alghoraishi M, Anvari M, Talebi AR, Amini-Rad O, Ghahramani R, Miresmaili SM. The effects of acrylamide on sperm parameters and membrane integrity of epididymal spermatozoa in mice. Eur J Obstet Gynecol Reprod Biol 2010; 153: 52-55. [DOI:10.1016/j.ejogrb.2010.07.008]
22. Amaral S, Oliveira PJ, Ramalho-Santos J. Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive species. Curr Diabetes Rev 2008; 4: 46-54. [DOI:10.2174/157339908783502398]
23. Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245-251. [DOI:10.1111/j.1439-0272.2008.00852.x]
24. Yang HJ, Lee SH, Jin Y, Choi JH, Han CH, Lee MH. Genotoxicity and toxicological effects of acrylamide on reproductive system in male rats. J Vet Sci 2005; 6: 103-109.
25. Wang H, Huang P, Lie T, Li J, Hutz RJ, Li K, et al. Reproductive toxicity of acrylamide-treated male rats. Reprod Toxicol 2010; 29: 225-230. [DOI:10.1016/j.reprotox.2009.11.002]
26. Song HX, Wang R, Geng ZM, Cao SX, Liu TZ. [Subchronic exposure to acrylamide affects reproduction and testis endocrine function of rats]. Zhonghua Nan Ke Xue 2008; 14: 406-410. (In Chinese)
27. Khalaj M, Abbasi AR, Nishimura R, Akiyama K, Tsuji T, Noguchi J, et al. Leydig cell hyperplasia in an ENU-induced mutant mouse with germ cell depletion. J Reprod Dev 2008; 54: 225-228. [DOI:10.1262/jrd.19191]
28. Paniagua R, Rodrı´guez MC, Nistal M, Fraile B, Regadera J, Amat P. Changes in surface area and number of Leydig cells in relation to the 6 stages of the cycle of the human seminiferous epithelium. Anat Embryol (Berl) 1988; 178: 423-427. [DOI:10.1007/BF00306048]
29. Calleman CJ. The metabolism and pharmacokinetics of acrylamide: implications for mechanisms of toxicity and human risk estimation. Drug Metab Rev 1996; 28: 527-590. [DOI:10.3109/03602539608994018]
30. Gamboa da Costa G, Churchwell MI, Hamilton LP, Von Tungeln LS, Beland FA, Marques MM, et al. DNA adduct formation from acrylamide via conversion to glycidamide in adult and neonatal mice. Chem Res Toxicol 2003; 16: 1328-1337. [DOI:10.1021/tx034108e]
31. Sega GA, Generoso EE. Measurement of DNA breakage in specific germ-cell stages of male mice exposed to acrylamide, using an alkaline-elution procedure. Mutat Res 1990; 242: 79-87. [DOI:10.1016/0165-1218(90)90101-7]
32. Dearfield KL, Douglas GR, Ehling UH, Moore MM, Ga GA, Brusick DJ. Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Mutat Res 1995; 330: 71-99. [DOI:10.1016/0027-5107(95)00037-J]
33. Tice RR, Andrews PW, Hire O, Singh NP. The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells. In: Biological Reactive Intermediates. IV Witmer CM, et al. New York, Plenum Press; 1990: 157-164.
34. Zhang JX, Yue WB, Ren YS, Zhang CX. Enhanced role of elaidic acid on acrylamide-induced oxidative stress in epididymis and epididymal sperm that contributed to the impairment of spermatogenesis in mice. Toxicol Ind Health 2010; 26: 469-477. [DOI:10.1177/0748233710373084]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb