Volume 12, Issue 7 (8-2014)                   IJRM 2014, 12(7): 459-0 | Back to browse issues page

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Askari Jahromi M, Movahedin M, Mazaheri Z, Amanlu M, Mowla S J, Batooli H. Evaluating the effects of Escanbil (Calligonum) extract on the expression level of Catsper gene variants and sperm motility in aging male mice. IJRM 2014; 12 (7) :459-0
URL: http://ijrm.ir/article-1-567-en.html
Evaluating the effects of Escanbil (Calligonum) extract on the expression level of Catsper gene variants and sperm motility in aging male mice
Mahsa Askari Jahromi1 , Mansoureh Movahedin * 2, Zohreh Mazaheri1 , Masoud Amanlu3 , Seyed Javad Mowla4 , Hosein Batooli5
1- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , movahed.m@modares.ac.ir
3- Department of Medicinal Chemistry, Faculty of Pharmacy, Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Genetic, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
5- Isfahan Research Center of Agriculture and Natural Resources, Kashan Botanical Garden, Kashan, Iran
Abstract:   (3237 Views)
Background: Catsper proteins are responsible for entering Ca2+ to the cell and play an important role in sperm motility and male fertility. Antioxidants are vital for sperm motility too. Escanbil (Calligonum) extract possess some of the important antioxidant like Catechin and Quercetin.
Objective: Here we investigated the effects of Escanbil (Calligonum) extract on the sperm parameters and the expressing of Catsper gene in aging male mice.
Materials and Methods: In this animal study, firstly, dose response was performed by using these three doses of Escanbil (Calligonum) (10, 30 and 50 mg/kg). 5 mice in each group were considered and Intra Peritoneal injection was done for 5 weeks. the sperm parameters analyzed and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL )staining was done. 30 mg/kg dose was considered as optimum dose. Secondly: fifteen aging male mice (11-13 months) were divided into three groups: control, sham and experiment. The experiments were injected Intra peritonealy with Escanbil (Calligonum) extract (30mg/kg) weekly for up to 5 weeks. The sham group was injected Intra Peritoneal (DMSO). Sperm parameters were analyzed. Expression of Catsper genes was analyzed by Real time PCR.
Results: Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05). Our data showed that there was a statistical significance difference between the expressions of Catsper 2, 4 in aging experiment group comparison with aging control group (p<0.05).
Conclusion: We investigated that the Escanbil (Calligonum) extract (30 mg/kg) can improve sperm parameters and change the expression of Catsper genes in aging male mice. This herbal extract can be used as an antioxidant component for clinical usages.
 
Keywords: Spermatozoa, Catsper protein, Antioxidants, Ca2+ channels
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Type of Study: Original Article |
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