International Journal of
Reproductive Biomedicine
Mon, Dec 23, 2024
[Archive]
Volume 12, Issue 8 (8-2014)
IJRM 2014, 12(8): 567-0 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Zhang H, Zhou Y, Li Y, Zheng Y, Xiao S, Wu Y, et al . Prediction of clinical pregnancy in vitrified-warmed single blastocyst transfer cycles by pre-freeze morphology. IJRM 2014; 12 (8) :567-0
URL: http://ijrm.ir/article-1-568-en.html
URL: http://ijrm.ir/article-1-568-en.html
Huan Zhang1 
, Ying Zhou1 , Yan Li1 , Yi Zheng1 , Shiquan Xiao1 , Yonggen Wu1 , Haiqing Wang1 , Xuefeng Huang * 2
, Ying Zhou1 , Yan Li1 , Yi Zheng1 , Shiquan Xiao1 , Yonggen Wu1 , Haiqing Wang1 , Xuefeng Huang * 2
1- Department of Reproductive Medical Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2- Department of Reproductive Medical Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China ,shuoz10@126.com
2- Department of Reproductive Medical Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China ,
Abstract: (2317 Views)
Background: The selection of blastocyst warmed for transfer is based on pre-freeze morphology in vitrified-warmed single blastocyst transfer cycles. But, it is controversial which parameter of blastocyst morphology most closely related to the clinical outcomes.
Objective: To estimate the effect of blastocoele expansion, trophectoderm (TE) morphology grade, and inner cell mass (ICM) morphology grade on clinical pregnancy in vitrified-warmed single blastocyst transfers.
Materials and Methods: There were 172 vitrified-warmed single blastocyst transfer cycles during the year 2012 included in this analysis. Comparison of clinical results between pregnancy and no pregnancy group based on patient and blastocyst morphology characteristics was done. Then stepwise logistic regression analysis was used to select the best morphological predictor for clinical pregnancy. Last, comparison of patient characteristics and clinical outcomes separated by the best independent morphological predictor was done.
Results: Comparison of clinical results between pregnancy and no pregnancy group and logistic regression showed the clinical pregnancy rate was affected by ICM. Comparison of patient characteristics separated by ICM grade, ICM grade A cycles got higher clinical pregnancy rate than ICM grade B cycles (54.3% vs. 35.0% respectively, p=0.037).
Conclusion: Blastocyst with good ICM morphology could increase clinical pregnancy rate in vitrified-warmed single blastocyst transfer cycles.
Objective: To estimate the effect of blastocoele expansion, trophectoderm (TE) morphology grade, and inner cell mass (ICM) morphology grade on clinical pregnancy in vitrified-warmed single blastocyst transfers.
Materials and Methods: There were 172 vitrified-warmed single blastocyst transfer cycles during the year 2012 included in this analysis. Comparison of clinical results between pregnancy and no pregnancy group based on patient and blastocyst morphology characteristics was done. Then stepwise logistic regression analysis was used to select the best morphological predictor for clinical pregnancy. Last, comparison of patient characteristics and clinical outcomes separated by the best independent morphological predictor was done.
Results: Comparison of clinical results between pregnancy and no pregnancy group and logistic regression showed the clinical pregnancy rate was affected by ICM. Comparison of patient characteristics separated by ICM grade, ICM grade A cycles got higher clinical pregnancy rate than ICM grade B cycles (54.3% vs. 35.0% respectively, p=0.037).
Conclusion: Blastocyst with good ICM morphology could increase clinical pregnancy rate in vitrified-warmed single blastocyst transfer cycles.
Type of Study: Original Article |
References
1. Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft WB. Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertil Steril 1998; 69: 84-88. [DOI:10.1016/S0015-0282(97)00438-X]
2. Gardner DK, Schoolcraft WB. In vitro culture of human blastocyst. In: Mortimer JR, editor. Toward Reproductive Certainty: Infertility and Genetics Beyond 1999. Carnforth, UK, Parthenon Press; 1999: 378-388.
3. Alpha Scientists in Reproductive Medicine and ESHRE Special Interes Group of Embryology. Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 2011; 26: 1270-1280. [DOI:10.1093/humrep/der037]
4. Van den Abbeel E, Balaban B, Ziebe S, Lundin K, Cuesta MJ, Klein BM, et al. Association between blastocyst morphology and outcome of single-blastocyst transfer. Reprod Biomed Online 2013; 27: 353-361. [DOI:10.1016/j.rbmo.2013.07.006]
5. Richter KS, Harris DC, Daneshmand ST, Shapiro BS. Quantitative grading of a human blastocyst: optimal inner cell mass size and shape. Fertil Steril 2001; 76: 1157-1167. [DOI:10.1016/S0015-0282(01)02870-9]
6. Kovacic B, Vlaisavljevic V, Reljic M, Cizek-Sajko M. Developmental capacity of different morphological types of day 5 human morulae and blastocysts. Reprod Biomed Online 2004; 8: 687-694. [DOI:10.1016/S1472-6483(10)61650-1]
7. Hill MJ, Richter KS, Heitmann RJ, Graham JR, Tucker MJ, DeCherney AH, et al. Trophectoderm grade predicts outcomes of single-blastocyst transfers. Fertil Steril 2013; 99: 1283-1289. [DOI:10.1016/j.fertnstert.2012.12.003]
8. Ahlström A, Westin C, Reismer E, Wikland M, Hardarson T. Trophectoderm morphology: an important parameter for predicting live birth after single blastocyst transfer. Hum Reprod 2011; 26: 3289-3296. [DOI:10.1093/humrep/der325]
9. Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Hudson C, Thomas S. Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and frozen-thawed embryo transfers in high responders. Fertil Steril 2011; 96: 516-518. [DOI:10.1016/j.fertnstert.2011.02.059]
10. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 2007; 67: 73-80. [DOI:10.1016/j.theriogenology.2006.09.014]
11. Endo T, Honnma H, Hayashi T, Chida M, Yamazaki K, Kitajima Y, et al. Continuation of GnRH agonist administration for 1 week, after hCG injection, prevents ovarian hyperstimulation syndrome following elective cryopreservation of all pronucleate embryos. Hum Reprod 2002; 17: 2548-2551. [DOI:10.1093/humrep/17.10.2548]
12. Kini S, Li HW, Morrell D, Pickering S, Thong KJ. Anti-mullerian hormone and cumulative pregnancy outcome in in-vitro fertilization. J Assist Reprod Genet 2010; 27: 449-456. [DOI:10.1007/s10815-010-9427-2]
13. Rossant J, Lis WT. Potential of isolated mouse inner cell masses to form trophectoderm derivatives in vivo. Dev Biol 1979; 70: 255-261. [DOI:10.1016/0012-1606(79)90022-8]
14. Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Thomas S, et al. Large blastocyst diameter, early blastulation, and low preovulatory serum progesterone are dominant predictors of clinical pregnancy in freah autologous cycles. Fertil Steril 2008; 90: 302-309. [DOI:10.1016/j.fertnstert.2007.06.062]
15. Marikawa Y, Alarcón VB. Establishment of trophectoderm and inner cell mass lineages in the mouse embryo. Mol Reprod Dev 2009; 76: 1019-1032. [DOI:10.1002/mrd.21057]
16. Ebner T, Vanderzwalmen P, Shebl O, Urdl W, Moser M, Zech NH, et al. Morphology of vitrified/warmed day-5 embryos predicts rates of implantation, pregnancy and live birth. Reprod Biomed Online 2009; 19: 72-78. [DOI:10.1016/S1472-6483(10)60049-1]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
