Volume 13, Issue 11 (11-2015)                   IJRM 2015, 13(11): 721-728 | Back to browse issues page

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Abbas T, Raees Ahmad K, Ullah A, Iqbal S, Raees K. Mitigating effects of Jambul against lead induced toxicity in epididymis and vas deferens of mice. IJRM 2015; 13 (11) :721-728
URL: http://ijrm.ir/article-1-602-en.html
Mitigating effects of Jambul against lead induced toxicity in epididymis and vas deferens of mice
Tahir Abbas * 1, Khawaja Raees Ahmad2 , Asmat Ullah3 , Samreen Iqbal2 , Kausar Raees4
1- Department of Biology, Government Degree College, Kotmomin Sargodha, Pakistan , tahirabbasbloch@gmail.com
2- University of Sargodha, Sargodha, Pakistan
3- University of the Punjab Lahore, Pakistan
4- Department of Biology, Government College for Women Farooq Colony Sargodha, Pakistan
Abstract:   (2679 Views)
Background: Precious fruits like jambul are neglected and wasted while environmental pollutants like lead intake remain overlooked. Objective: The aim of this study was to investigate the effects of the Jambul pulp extract on lead detrimental effects in pseudostratified epithelium and the stereocilia of mice epididymis and vas deferens. Materials and Methods: Thirty young males mice (Mus musculus) were distributed randomly in 3 groups (n= 10) called control, Pb (Lead) and Pb-J (Lead-Jambul). The Pb and Pb-J were provided 50ppm Pb in drinking water ad libitum for 15 days and Pb free water for the next 5 days. The Pb-J group received 0.2ml jambul pulp extract on 12 hourly bases. Control group was not given any treatment. Organs (epididymis and vas deference) were recovered on 21st day after euthanasia. The organs were finally processed for histological and micrometric studies. Results: Marked histologic and micrometric changes in both organs were noted in Pb group. These include significant (P ≤ 0.05) decrease in cross sectional area of caput and cauda epididymis folding tubing along with evident alterations of their endothelial thickness. Prominent signs of apoptosis (vacuolations) in the corpus pseudostratified endothelium and the destruction of stereocilia of the epididymis and vas deferens in Pb compared to control group were observed. Evident signs of recovery, in both organs, such as proliferation and rearrangements in pseudostratified endothelium and the stereocilia along with convincing recovery in micrometric parameters were observed in Pb-J group. Conclusion: The results indicate that epididymis and vas deferens are highly sensitive to Pb exposure while Jambul pulp extract has shown rich mitigating potentials against such histopathologies.
Keywords: Epididymis, Vas deferens, Apoptosis, Stereocilia, Jambul.
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Type of Study: Original Article |
References
1. Freer-Smith P, Carnus JM. The sustainable management and protection of forests analysis of the current position globally. Ambio 2008; 37: 254-262. [DOI:10.1579/0044-7447(2008)37[254:TSMAPO]2.0.CO;2]
2. Yang YQ, Chen FR, Zhang L, Liu J, Wu S, Kang M. Comprehensive assessment of heavy metal contamination in sediment of the Pearl River Estuary and adjacent shelf. Mar Pollut Bull 2012; 64: 1947-1955. [DOI:10.1016/j.marpolbul.2012.04.024]
3. Florea AM, Busselberg D. Occurrence use and potential toxic effects of metals and metal compounds. Biometals 2006; 19: 419-427. [DOI:10.1007/s10534-005-4451-x]
4. Mahdy TM, Giorgi T, Adewole F, Ernest I, Idoko M, Matey N, et al. Effect of Moringa oleifera, activated carbon and wood charcoal on biochemical and hematological parameters of Wistar rats exposed to lead acetate. Med Weter 2012; 68: 101-106.
5. De Marco M, Halpern R, Barros HM. Early behavioral effects of lead perinatal exposure in rat pups. J Toxicol 2005; 211: 49-58. [DOI:10.1016/j.tox.2005.02.007]
6. Pizzol M, Thomsen M, Anderson MS. Long-term Human Exposure to Lead from Different Media and intake Pathways. Sci Total Environ 2010; 408: 5478-5488. [DOI:10.1016/j.scitotenv.2010.07.077]
7. Kojima M, Sekikawa K, Nemoto K, Degawa M. Tumor Necrosis Factor-α-Independent down regulation of hepatic cholesterol 7α-Hydroxylase gene in mice treated with Lead Nitrate. Toxicol Sci 2005; 87: 537-542. [DOI:10.1093/toxsci/kfi267]
8. Bomfim KMA, Garcia CAB, Reis FP, Palmeira RJAV, Scher R, Aragao JA. Absorption levels and morphological features of fetal organs in wistar rats treated with lead acetate. Int J Morphol 2012; 30: 119-124. [DOI:10.4067/S0717-95022012000100021]
9. Hamadouche NA, Sadi N, Kharoubi O, Slimani M, Aoues A. The protective effect of Vitamin E against genotoxicity of Lead Acetate intraperitoneal administration in male rat. Arch Biol Sci Belgrade 2013; 65: 1435-1445. [DOI:10.2298/ABS1304435H]
10. Lie C, Xie B. Evaluation of the antioxidant pro-oxidanteffects of tea catechin oxypolymers. J Agric Food Chem 2000; 48: 6362-6366. [DOI:10.1021/jf000110p]
11. Gulcin I. The antioxidant and radical scavenging activities of black pepper seeds. Int J Food Sci Nutr 2005; 56: 491-499. [DOI:10.1080/09637480500450248]
12. Gebicka L, Banasiak E. Flavonoids as reductants of ferryl hemo- globin. Acta Biochim Pol 2009; 56: 509-513.
13. Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol 2011; 3: 10-14.
14. Moshi MJ, Otieno DF, Weisheit A. Ethnomedicine of the Kagera Region, North Western Tanzania. Part 3: plants used in traditional medicine in Kikuku village, Muleba District. J Ethnobiol Ethnome 2012; 8: 14. [DOI:10.1186/1746-4269-8-14]
15. Abdel-Hameed ES, Bazaid SA, Shohayeb MM, El-Sayed MM, El-Wakil EA. Phytochemical studies and evaluation of antioxidant, anticancer and antimicrobial properties of Conocarpus erectus L. growing in Taif, Saudi Arabia. Europ J Med Plants 2012; 2: 93–112. [DOI:10.9734/EJMP/2012/1040]
16. Gordon A, Jungfer E, Da-Silva BA, Maia JG, Marx F. Phenolic constituents and antioxidant capacity of four underutilized fruits from the amazonregion. J Agric Food Chem 2011; 59: 7688-7699. [DOI:10.1021/jf201039r]
17. Venkateswarlu G. On the nature of the colouring matter of the jambul fruit (Eugenia jambolana). J Indian Chem Soc 1952; 29: 434-437.
18. Sengupta P, Das PB. Terpenoids and related compunds part IV triterpenoids the stem-bark of Eugenia jambolana Lam. Indian Chem Soc 1965; 42: 255-258.
19. Soncharan P, Shanmugarajan TS, Somasundaram, Niladri M. Protective effect of Syzygium cumini seeds against doxorubicin induced cardiotoxicity in rats. Int J Phar Life Sci 2010; 6: 343-349.
20. Ahmad KR, Nauroze T, Raees K, Abbas T, Kanwal MA, Noor S, et al. Protective role of jambul (Syzygium cumini) fruit-pulp extract against fluoride-induced toxicity in mice testis: a histopathologiocal study. Flouride 2012: 45: 281-289.
21. Bancroft JD, Gamble M. Theory and Practice of histological techniques, 5th Ed. New York: Churchill Livingstone.2003; 593-620.
22. Bharali MR. Effect of acute lead acetate exposure on liver of mice. Journal of Global Biosciences 2013; 2: 121-125.
23. Xin-hong L, Zhi-ying W, Li Qin Zhen-liang C, Xu Y. Effects of cadmium on testis spermatocyte apoptosis and epididymis mature spermatozoa quality of mice. Acad J 2010; 21: 987-992.
24. Shum WW, Smith TB, Cortez-Retamozo V, Grigoryeva LS, Roy JW, Hill E, et al. Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis. Biolo Reprod 2014; 90: 90. [DOI:10.1095/biolreprod.113.116681]
25. Sharma R, Garu U. Effects of lead toxicity on developing testes in Swiss mice. Univ J Environ Res Technol 2011; 1: 390-398.
26. Singh P, Deora K, Bano H, Mogra P, Javeria S, Barolia S. Protective effect of curcumin on cadmium chloride induced epididymal toxicity in Swiss mice (Mus musculus). J Chem Bio Phy Sci Sec 2012; 2: 797-805.
27. Chinoy NJ, Momin R, Jhala DD. Fluoride and Aluminium induced toxicity in Mice Epididymis and its mitigation by Vitamin C. Fluoride 2005; 38: 115–121.
28. Young-Ok SJ, Hitron A, Wang X, Chang Q, Pan J. Cr (VI) induces mitochondrial-mediated and caspase-dependent apoptosis through reactive oxygen species-mediated p53 activation in JB6 Cl41 cells. Toxicol App Pharmacol 2010; 245: 226-235. [DOI:10.1016/j.taap.2010.03.004]
29. Fatima S, Mahmood R. Vitamin C attenuates potassium dichromate-induced nephrotoxicity and alterations in renal brush border membrane enzymes and phosphate transport in rats. Clin Chim Acta 2007; 386: 94-99. [DOI:10.1016/j.cca.2007.08.006]
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