Volume 13, Issue 4 (5-2015)                   IJRM 2015, 13(4): 209-214 | Back to browse issues page

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Nematollahi Z, Hadinedoushan H, Aflatoonian A, Eslami G, Ghasemi N. The association between single nucleotide polymorphism in interleukin-27 gene and recurrent pregnancy loss in Iranian women. IJRM 2015; 13 (4) :209-214
URL: http://ijrm.ir/article-1-642-en.html
The association between single nucleotide polymorphism in interleukin-27 gene and recurrent pregnancy loss in Iranian women
Zeinab Nematollahi1 , Hossein Hadinedoushan * 2, Abbas Aflatoonian3 , Gilda Eslami4 , Nasrin Ghasemi3
1- Department of Immunology, Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Immunology, Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , hhadin@ssu.ac.ir
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (2967 Views)
Background: Recurrent pregnancy loss (RPL) has been defined as two or more miscarriages before 20 th week of gestation. It seems that IL-27 may reduce inflammatory responses and affect the survival of the embryo during human pregnancy. IL-27 polymorphisms may influence RPL by altering the levels or the activity of gene product.
Objective: We studied for the first time the association of IL-27 -964 A>G single nucleotide polymorphism (SNP) with RPL in Iranian women.
Materials and Methods: A case-controlled study was performed on two groups consisting of 150 healthy women with at least one delivery (control group) and 150 women with two or more primary RPLs history (RPL group). The -964 A>G SNP in IL-27 gene was determined by PCR-RFLP technique. Genotype and allele frequencies were compared using c 2 tests between two groups.
Results: There was no difference between the two groups regarding age of women (29±4.4 [control] vs. 30.84±5.2 years [case]). In the RPL group, the genotype frequencies of -964 A>G polymorphism were AG (49.3%), AA (40%), and GG (10.7%), and in the control group, they were AG (43.3%), AA (48.7%), and GG (8%). There was no significant difference between the genotypes of AA, AG, and GG in two groups (p=0.23). As the frequency of allele A was 64.7% in the RPL group and 70.3% in the control group, the difference in frequency of allele A in -964 A>G between two groups was not significant (p=0.19).
Conclusion: Our findings indicate that SNP of -964 A>G in IL-27 gene is not a risk factor for RPL in Iranian women.
Keywords: Cytokine, IL-27, Inflammation, Polymorphism, Recurrent abortion.
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Type of Study: Original Article |
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