Volume 13, Issue 9 (10-2015)                   IJRM 2015, 13(9): 525-532 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosen B, Islam R, Begum F, Kabir Y, Howlader Z H. Oxidative stress induced sperm DNA damage, a possible reason for male infertility. IJRM 2015; 13 (9) :525-532
URL: http://ijrm.ir/article-1-681-en.html
1- Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh
2- Department of Obstetrics and Gynecology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka-1000, Bangladesh
3- Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh , hhzakir@yahoo.com
Abstract:   (2886 Views)
Background: Sperm DNA damage is an important factor in the etiology of male infertility.
Objective: The aim of the study was to evaluate the association of oxidative stress induced sperm DNA damage with the pathogenesis of male infertility.
Materials and Methods: The study comprised a total of 66 subjects, including fertile men (n=25) and infertile men (n=41) matched by age. Seminal malondialdehyde (MDA), phospholipid hydroperoxide (PHP), superoxide dismutase (SOD), total antioxidant status (TAS) and 8-hydroxy-2'-deoxy guanosine (8-OHdG) were estimated by spectrophotometric and ELISA based methods and the association with the sperm parameters was assessed.
Results: The percentages of motile and morphologically normal cells were significantly lower (p < 0.001, p <0.001, respectivly) in infertile men. Seminal levels of MDA, PHP and 8-OHdG were significantly higher (p < 0.001, p < 0.001, and p=0. 02, respectively) while the SOD and TAS were significantly lower (p=0. 0003, p< 0.001, respectively) in infertile men. Sperm parameters were negatively correlated with MDA, PHP and 8-OHdG while positively correlated with SOD and TAS. A positive correlation of 8-OHdG with MDA and PHP and a negative correlation with TAS and SOD were also found.
Conclusion: These results suggested that oxidative stress induced sperm DNA damage might have a critical effect on the etiology of infertility. Therefore, evaluation of oxidative status, antioxidant defense systems and DNA damage, together with sperm parameters might be a useful tool for diagnosis and treatment of male infertility.
Full-Text [PDF 758 kb]   (867 Downloads) |   |   Full-Text (HTML)  (1035 Views)  
Type of Study: Original Article |

1. Zeba UN, Ali M, Biswas SK, Kamrun N, Bashar T, Arslan MI. Study of seminal MDA level as an oxidative stress. J Sci Foundation 2011; 9: 85-93.
2. Bashed MA, Mahabubul GA, Kabir MA, Al-Amin AQ. Male Infertility in Bangladesh: What Serve Better-Pharmacological Help or Awareness Programme? Int J Pharmacol 2012; 8: 687-694. [DOI:10.3923/ijp.2012.687.694]
3. Campbell AJ, Irvine DS. Male infertility and intracytoplasmic sperm injection. Br Med Bull 2000; 56: 616-629. [DOI:10.1258/0007142001903427]
4. Rowe PJ, Comhaire FH, Hargreave TB, Mahmoud AMA. WHO manual for the Standardized investigations, diagnosis and management of infertile couple. 4th Ed. Cambridge: Cambridge University Press. World Health Organization, 2000.
5. Sidhu RS, Hallak J, Sharma RK, Thomas AJ, Agarwal A. Relationship of creatine kinase levels with clinical diagnosis of infertility. J Assist Reprod Genet 1998; 15: 188–192. [DOI:10.1023/A:1023096201880]
6. Keel BA. The semen analysis. CRC handbook of the laboratory diagnosis and treatment of infertility. In: Webster, B.W. (Eds). Boca Raton, FL: CRC Press. 1990; 27-69.
7. Aitken RJ, Clarkson JS, Fishel S. Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biol Reprod 1989; 40: 183–197. [DOI:10.1095/biolreprod41.1.183]
8. Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992; 57: 409 –416. [DOI:10.1016/S0015-0282(16)54855-9]
9. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urology 1996; 48: 835–850. [DOI:10.1016/S0090-4295(96)00313-5]
10. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002; 29: 817-827. [DOI:10.1016/S0094-0143(02)00081-2]
11. Agarwal A, Allamaneni SS. Role of free radicals in female reproductive diseases and assisted reproduction. Reprod Biomed Online 2004; 9: 338-347. [DOI:10.1016/S1472-6483(10)62151-7]
12. Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod 1999; 4: 31-37. [DOI:10.1530/ror.0.0040031]
13. Said TMS, Agarwal A, Sharma RK, Thomas AJ, Sikka SCJ. Impact of sperm morphology on DNA damage caused by oxidative stress induced by beta-nicotinamide adenine dinucleotide phosphate. Fertil Steril 2005; 83: 95-103. [DOI:10.1016/j.fertnstert.2004.06.056]
14. Shen H, Ong C. Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility. Free Radic Biol Med 2000; 28: 529–536. [DOI:10.1016/S0891-5849(99)00234-8]
15. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th Ed. Geneva: World Health Organization, 2010.
16. Yagi K. Simple procedure for specific assay of lipid hydroperoxides in serum or plasma. Methods Mol Biol 1998; 108: 101-106. [DOI:10.1385/0-89603-472-0:107]
17. Miyazawa T. Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay. Free Radic Biol Med 1989; 165: 988-993. [DOI:10.1016/0891-5849(89)90017-8]
18. Peskin AV, Winterbourn CC. A microtiter plate assay for superoxide dismutase using a water-olubletetrazolium salt (WST-1). Clin Chim Acta 2000; 293: 157–166. [DOI:10.1016/S0009-8981(99)00246-6]
19. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 53: 1841-1856. [DOI:10.1021/jf030723c]
20. Shen J, Deininger P, Hunt JD, Zhao H. 8-hydroxy-2′-deoxyguanosine as apotential survival biomarkers in patients with non-small cell lung cancer. Cancer 2007; 109: 574-580. [DOI:10.1002/cncr.22417]
21. Saleh RA, Agarwal A. Oxidative stress and male infertility: from research bench to clinical practice. J Androl 2002; 23: 737-752.
22. Khosrowbeygi A, Zarghami N. Levels of oxidative stress biomarkers in seminal plasma and their relationship with seminal parameters. BMC Clin Pathol 2007; 7: 6. [DOI:10.1186/1472-6890-7-6]
23. Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ, Agarwal A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing fertility investigation. Fertil Steril 2000; 73: 459-464. [DOI:10.1016/S0015-0282(99)00567-1]
24. Nabil H, Lekaka A, Moemen MH, Eela A. Studying the Levels of Malondialdehyde and Antioxidant Parameters in Normal and Abnormal Human Seminal Plasma. Australian Journal of Basic and Applied Sciences 2008; 2: 773-778.
25. Hsieh YY, Chang C, Lin CS. Seminal malondialdehyde concentra‌tion but not glutathione peroxidase activity is negatively cor‌related with seminal concentration and motility. Int J Biol Sci 2006; 2: 23-29. [DOI:10.7150/ijbs.2.23]
26. Sanocka D, Miesel R, Jedrzejczak P, Chelmonska-Soyta AC, Kurpisz M. Effect of reactive oxygen species and the activity of antioxidant systems on human semen; association with male infertility. Int J Androl 1997; 20: 255–264. [DOI:10.1046/j.1365-2605.1997.00050.x]
27. Siciliano L, Tarantino P, Longobardi F, Rago V, Stefano, DC, Carpino A. Impaired seminal antioxidant capacity in human semen with hyperviscosity or oligoasthenozoospermia. J Androl 2001; 22: 798-803.
28. Murawski M, Saczko J, Marcinkowska A, Chwikowska A, Grybooe M, Banaoe T. Evaluation of superoxide dismutase activity and its impact on semen quality parameters of infertile men. Folia Histochemt Cytobiol 2007; 45: 123-126.
29. Badade G, More K, Narshetty G, Badade VZ, Yadav B. Human semi‌nal oxidative stress: correlation with antioxidants and sperm quality parameters. Annal Biol Res 2011; 2: 351-359.
30. Khosrowbeygi A, Zarghami N, Deldar Y. Cor‌relation between sperm quality parameters and seminal plasma antioxidants status. Iran J Reprod Med 2012; 2: 58-64.
31. Duru NK, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermato‌zoa. Fertil Steril 2000; 74: 1200-1207. [DOI:10.1016/S0015-0282(00)01591-0]
32. Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 1997; 68: 519–524. [DOI:10.1016/S0015-0282(97)00236-7]
33. Aksoy H, Aksoy Y, Ozbey I, Altuntas I, Akcay F. The relationship between varicocele and semen nitric oxide concentrations. Urol Res 2000; 28: 357–359. [DOI:10.1007/s002400000132]
34. Huang YL, Tseng WC, Cheng SY, Lin TH. Trace elements and lipid peroxidation in human seminal plasma. Biol Trace Elem Res 2000; 76: 207-215. [DOI:10.1385/BTER:76:3:207]
35. Asbagh AF, Mostafavi E, Hamdi K, Azmodeh O, Ghasemyne‌jad A, Moshtaghi J. Relation of serum and semen malondial‌dehyde and total anti-oxidants with sperm parameters in infer‌tile men. Am J Immunolo 2010; 6: 43-49. [DOI:10.3844/ajisp.2010.43.49]
36. Patel S, Panda S, Nanda R, Mangaraj M, Mohapatra PC. Influence of oxidants and anti-oxidants on semen parameters in infertile males. J Indian Med Assoc 2009; 107: 78-80.
37. Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2003; 49: 83-94. [DOI:10.1080/01485010390129269]
38. Ni ZY, Liu YQ, Shen HM, Chia SE, Ong CN. Does the increase of 8-hydroxydeoxyguanosine lead to poor sperm quality? Mutat Res 1997; 381: 77–82. [DOI:10.1016/S0027-5107(97)00151-6]

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb