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Reproductive Biomedicine
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Volume 14, Issue 1 (1-2016)
IJRM 2016, 14(1): 15-22 |
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Kargar- Dastjerdy P, Tavalaee M, Salehi M, Falahati M, Izadi T, Nasr Esfahani M H. Altered expression of KLC3 may affect semen parameters. IJRM 2016; 14 (1) :15-22
URL: http://ijrm.ir/article-1-691-en.html
URL: http://ijrm.ir/article-1-691-en.html
Pegah Kargar- Dastjerdy * 
1, Marziyeh Tavalaee1 , Mansoor Salehi2 , Mojtaba Falahati3 , Tayebeh Izadi4 , Mohammad Hossein Nasr Esfahani5
1, Marziyeh Tavalaee1 , Mansoor Salehi2 , Mojtaba Falahati3 , Tayebeh Izadi4 , Mohammad Hossein Nasr Esfahani5
1- Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
2- Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
4- Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
5- Isfahan Fertility and Infertility Center, Isfahan, Iran
2- Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
4- Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
5- Isfahan Fertility and Infertility Center, Isfahan, Iran
Abstract: (3470 Views)
Background: KLC3 protein as a member of the kinesin light-chain protein family plays an important role in spermatogenesis, during formation of mitochondrial sheath in the mid piece of the sperm tail.
Objective: This study for the first time aims to compare the expression of the KLC3 gene between fertile and infertile individuals.
Materials and Methods: Semen samples were collected from 19 fertile individuals who were selected from embryo-donor volunteers and 57 infertile individuals who had abnormal sperm parameters according to world health organization criteria. Sperm parameters using computer assisted sperm analysis and the quantitative KLC3-gene expression using the real-time PCR method were measured.
Results: Our results revealed a significant correlations between sperm concentration with relative expression of KLC3 only in infertile groups (r=0.45, p=0.00). A significant correlation was not found between KLC3 expression and sperm motility; however, the relative expression of KLC3 was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals.
Conclusion: Low expression of KLC3 may result in improper function of midpiece, which has important function in sperm motility. The results of this study show that aberrant expression of KLC3 might be associated with phenomena like oligozoospermia and asthenozoospermia. This article is extracted from student’s thesis.
Objective: This study for the first time aims to compare the expression of the KLC3 gene between fertile and infertile individuals.
Materials and Methods: Semen samples were collected from 19 fertile individuals who were selected from embryo-donor volunteers and 57 infertile individuals who had abnormal sperm parameters according to world health organization criteria. Sperm parameters using computer assisted sperm analysis and the quantitative KLC3-gene expression using the real-time PCR method were measured.
Results: Our results revealed a significant correlations between sperm concentration with relative expression of KLC3 only in infertile groups (r=0.45, p=0.00). A significant correlation was not found between KLC3 expression and sperm motility; however, the relative expression of KLC3 was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals.
Conclusion: Low expression of KLC3 may result in improper function of midpiece, which has important function in sperm motility. The results of this study show that aberrant expression of KLC3 might be associated with phenomena like oligozoospermia and asthenozoospermia. This article is extracted from student’s thesis.
Type of Study: Original Article |
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