Volume 15, Issue 2 (3-2017)                   IJRM 2017, 15(2): 93-100 | Back to browse issues page

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Gaeini A, Baghaban Eslaminejad M, Choobineh S, Mousavi N, Satarifard S, Shafieineek L. Effects of exercise prior or during pregnancy in high fat diet fed mice alter bone gene expression of female offspring: An experimental study. IJRM 2017; 15 (2) :93-100
URL: http://ijrm.ir/article-1-799-en.html
Effects of exercise prior or during pregnancy in high fat diet fed mice alter bone gene expression of female offspring: An experimental study
Abbasali Gaeini1 , Mohamadreza Baghaban Eslaminejad2 , Siroos Choobineh1 , Neda Mousavi3 , Sadegh Satarifard1 , Leila Shafieineek * 4
1- Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
2- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
3- Department of Biochemistry and Nutrition, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
4- Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran , shafieineek@gmail.com
Abstract:   (2857 Views)
Background: Based on different studies it was shown that exercise training is an important factor in preconception and prenatal care.
Objective: Based on the importance of exercise training in preconception and perinatal careT, the aim of this study was to determine whether regular preconception exercise training with or without exercise training during pregnancy decreases detrimental effects of maternal high fat diet on female offspring bone health.
Materials and Methods: Twenty four C57BL/6 female mice were fed high fat diet (35%) and were randomly divided into four subgroups: trained in preconception period and exercised during pregnancy (TE); trained in preconception periods but unexercised during pregnancy (TC); untrained in preconception periods but exercised during pregnancy (CE); untrained and unexercised (CC). Trained mice were subjected to a protocol of moderate endurance exercise training over a period of 4 weeks before pregnancy. TE and CE Dams groups had access to wheels throughout pregnancy until delivery. Analyses were performed on the female offspring that did not have access to running wheels or exercise training during any portion of their lives. The relative expression levels of β-catenin, PPARƴ, OPG and RANKL were determined by Quantitative RT-PCR (qPCR).
Results: Exercise during pregnancy in isolation had no effect on any measure genes but exercise both before and during pregnancy affected all genes. Exercise only before pregnancy increased β-catenin and OPG and decreased PPARƴ, RANKL and RANKL/OPG ratio (p<0.001).
Conclusion: This study demonstrated that maternal exercise training before and during pregnancy may modulate the risk of bone disorders in offspring of mothers fed a high fat diet.
Keywords: Preconception care, Exercise, High fat diet, Offspring, Bone.
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Type of Study: Original Article |
References
1. McDonald SD, Han Z, Mulla S, Beyene J. Overweight and obesity in mothers and risk of preterm birth and low birth weight infants: systematic review and meta-analyses. BMJ 2010; 341: c3428. [DOI:10.1136/bmj.c3428]
2. Mingrone G, Manco M, Mora MEV, Guidone C, Iaconelli A, Gniuli D, et al. Influence of maternal obesity on insulin sensitivity and secretion in offspring. Diabetes care 2008; 31: 1872-1876. [DOI:10.2337/dc08-0432]
3. Dabelea D, Mayer-Davis EJ, Lamichhane AP, D'Agostino RB, Liese AD, Vehik KS, et al. Association of intrauterine exposure to maternal diabetes and obesity with type 2 diabetes in youth the SEARCH case-control study. Diabetes care 2008; 31: 1422-1446. [DOI:10.2337/dc07-2417]
4. Hanson MA, Gluckman PD. Developmental processes and the induction of cardiovascular function: conceptual aspects. J Physiol 2005; 565: 27-34. [DOI:10.1113/jphysiol.2004.082339]
5. Barker DJ. Fetal origins of coronary heart disease. BMJ 1995; 311: 171. [DOI:10.1136/bmj.311.6998.171]
6. Ge Z-J, Zhang C-L, Schatten H, Sun Q-Y. Maternal diabetes mellitus and the origin of non-communicable diseases in offspring: the role of epigenetics. Biol Reprod 2014; 90: 139. [DOI:10.1095/biolreprod.114.118141]
7. Winsloe C, Earl S, Dennison EM, Cooper C, Harvey NC. Early life factors in the pathogenesis of osteoporosis. Curr Osteop Rep 2009; 7: 140-144. [DOI:10.1007/s11914-009-0024-1]
8. Martyn-St James M, Carroll S. Meta-analysis of walking for preservation of bone mineral density in postmenopausal women. Bone 2008; 43: 521-531. [DOI:10.1016/j.bone.2008.05.012]
9. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143-147. [DOI:10.1126/science.284.5411.143]
10. Akune T, Ohba S, Kamekura S, Yamaguchi M, Chung U-i, Kubota N, et al. PPAR γ insufficiency enhances osteogenesis through osteoblast formation from bone marrow progenitors. J Clin Invest 2004; 113: 846. [DOI:10.1172/JCI200419900]
11. Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, et al. PPARγ is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 1999; 4: 611-617. [DOI:10.1016/S1097-2765(00)80211-7]
12. Boyce BF, Xing L. Functions of RANKL/RANK/OPG in bone modeling and remodeling. Arch Biochem Biophys 2008; 473: 139-146. [DOI:10.1016/j.abb.2008.03.018]
13. Khosla S. Minireview: The opg/rankl/rank system. Endocrinology 2001; 142:5050-5055. [DOI:10.1210/endo.142.12.8536]
14. Wan Y, Chong L-W, Evans RM. PPAR-γ regulates osteoclastogenesis in mice. Nat Med 2007; 13: 1496-1503. [DOI:10.1038/nm1672]
15. Bennett CN, Longo KA, Wright WS, Suva LJ, Lane TF, Hankenson KD, et al. Regulation of osteoblastogenesis and bone mass by Wnt10b. Proc Nat Acad Sci U S A 2005; 102: 3324-3329. [DOI:10.1073/pnas.0408742102]
16. Kang S, Bennett CN, Gerin I, Rapp LA, Hankenson KD, MacDougald OA. Wnt signaling stimulates osteoblastogenesis of mesenchymal precursors by suppressing CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor γ. J Biol Chem 2007; 282: 14515-14524. [DOI:10.1074/jbc.M700030200]
17. Chen J-R, Lazarenko OP, Wu X, Tong Y, Blackburn ML, Shankar K, et al. Obesity reduces bone density associated with activation of PPARgamma and suppression of Wnt/beta-catenin in rapidly growing male rats. PloS One 2010; 5: e13704. [DOI:10.1371/journal.pone.0013704]
18. Oken E, Gillman MW. Intervention strategies to improve outcome in obese pregnancies: focus on gestational. Matern Obes 2012: 151.
19. Weissgerber TL, Wolfe LA, Davies G. The role of regular physical activity in preeclampsia prevention. Med Sci Sports Exercise 2004; 36: 2024-2031. [DOI:10.1249/01.MSS.0000147627.35139.DC]
20. Sankaralingam S, Jiang Y, Davidge ST, Yeo S. Effect of exercise on vascular superoxide dismutase expression in high-risk pregnancy. Am J Perinatol 2011; 28: 803-810. [DOI:10.1055/s-0031-1284230]
21. Newcomer S, Taheripour P, Bahls M, Sheldon R, Foust K, Bidwell C, et al. Impact of porcine maternal aerobic exercise training during pregnancy on endothelial cell function of offspring at birth. J Dev Origins Health Dis 2012; 3: 4-9. [DOI:10.1017/S2040174411000687]
22. da Silva SG, de Almeida AA, Fernandes J, Lopim GM, Cabral FR, Scerni DA, et al. Maternal Exercise during Pregnancy Increases BDNF Levels and Cell Numbers in the Hippocampal Formation but Not in the Cerebral Cortex of Adult Rat Offspring. PloS one 2016; 11: e0147200. [DOI:10.1371/journal.pone.0147200]
23. Blaize AN, Pearson KJ, Newcomer SC. Impact of maternal exercise during pregnancy on offspring chronic disease susceptibility. Exercise Sport Sci Rev 2015; 43: 198-203. [DOI:10.1249/JES.0000000000000058]
24. Stanford KI, Lee M-Y, Getchell KM, So K, Hirshman MF, Goodyear LJ. Exercise before and during pregnancy prevents the deleterious effects of maternal high-fat feeding on metabolic health of male offspring. Diabetes 2015; 64: 427-433. [DOI:10.2337/db13-1848]
25. Rosa BV, Blair HT, Vickers MH, Dittmer KE, Morel PC, Knight CG, et al. Moderate Exercise during Pregnancy in Wistar Rats Alters Bone and Body Composition of the Adult Offspring in a Sex-Dependent Manner. PloS one 2013; 8: e82378. [DOI:10.1371/journal.pone.0082378]
26. Council NR. Nutrient Requirement of Laboratory Animals. 4th Ed. Washington DC, USA, National Academy Press; 1995.
27. Billiat V. Inter- and intrastrain variation in mouse critical running speed. J Appl Physiol 2005; 98: 1258-1263. [DOI:10.1152/japplphysiol.00991.2004]
28. Ireland D. Analysis of gene expression in bone by quantitative RT-PCR. Bone research protocols: Springer 2003: 433-440.
29. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 2001; 25: 402-408. [DOI:10.1006/meth.2001.1262]
30. Pate RR, Pratt M, Blair SN, Haskell WL, Macera CA, Bouchard C, et al. Physical activity and public health: a recommendation from the Centers for Disease Control and Prevention and the American College of Sports Medicine. JAMA 1995; 273: 402-407. [DOI:10.1001/jama.1995.03520290054029]
31. Bucay N, Sarosi I, Dunstan CR, Morony S, Tarpley J, Capparelli C, et al. Osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification. Gen Dev 1998; 12: 1260-1268. [DOI:10.1101/gad.12.9.1260]
32. Guadalupe-Grau A, Fuentes T, Guerra B, Calbet JA. Exercise and bone mass in adults. Sports Med 2009; 39: 439-468. [DOI:10.2165/00007256-200939060-00002]
33. Camozzi V, Tossi A, Simoni E, Pagani F, Francucci C, Moro L. Role of biochemical markers of bone remodeling in clinical practice. J Endocrinol Invest 2006; 30: 13-17.
34. Saunders M, Taylor A, Du C, Zhou Z, Pellegrini V, Donahue H. Mechanical stimulation effects on functional end effectors in osteoblastic MG-63 cells. J Biomech 2006; 39: 1419-1427. [DOI:10.1016/j.jbiomech.2005.04.011]
35. Hopkins SA, Cutfield WS. Exercise in pregnancy: weighing up the long-term impact on the next generation. Exercise Sport Sci Rev 2011; 39: 120-127. [DOI:10.1097/JES.0b013e31821a5527]
36. Clapp JF, Kim H, Burciu B, Schmidt S, Petry K, Lopez B. Continuing regular exercise during pregnancy: effect of exercise volume on fetoplacental growth. Am J Obstet Gynecol 2002; 186: 142-147. [DOI:10.1067/mob.2002.119109]
37. Varghese AC, Ly KD, Corbin C, Mendiola J, Agarwal A. Oocyte developmental competence and embryo development: impact of lifestyle and environmental risk factors. Reprod Biomed Online 2011; 22: 410-420. [DOI:10.1016/j.rbmo.2010.11.009]
38. McBride CM EK, Lipkus IM. Understanding the potential of teachable moments: the case of smoking cessation. Health Educ Res 2003; 18: 156-170. [DOI:10.1093/her/18.2.156]
39. Morris MJ, Chen H. Established maternal obesity in the rat reprograms hypothalamic appetite regulators and leptin signaling at birth. Int J Obes 2009; 33: 115-122. [DOI:10.1038/ijo.2008.213]
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