Volume 15, Issue 2 (3-2017)                   IJRM 2017, 15(2): 93-100 | Back to browse issues page

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Gaeini A, Baghaban Eslaminejad M, Choobineh S, Mousavi N, Satarifard S, Shafieineek L. Effects of exercise prior or during pregnancy in high fat diet fed mice alter bone gene expression of female offspring: An experimental study. IJRM 2017; 15 (2) :93-100
URL: http://ijrm.ir/article-1-799-en.html
Effects of exercise prior or during pregnancy in high fat diet fed mice alter bone gene expression of female offspring: An experimental study
Abbasali Gaeini1 , Mohamadreza Baghaban Eslaminejad2 , Siroos Choobineh1 , Neda Mousavi3 , Sadegh Satarifard1 , Leila Shafieineek * 4
1- Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
2- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
3- Department of Biochemistry and Nutrition, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
4- Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran , shafieineek@gmail.com
Abstract:   (3108 Views)
Background: Based on different studies it was shown that exercise training is an important factor in preconception and prenatal care.
Objective: Based on the importance of exercise training in preconception and perinatal careT, the aim of this study was to determine whether regular preconception exercise training with or without exercise training during pregnancy decreases detrimental effects of maternal high fat diet on female offspring bone health.
Materials and Methods: Twenty four C57BL/6 female mice were fed high fat diet (35%) and were randomly divided into four subgroups: trained in preconception period and exercised during pregnancy (TE); trained in preconception periods but unexercised during pregnancy (TC); untrained in preconception periods but exercised during pregnancy (CE); untrained and unexercised (CC). Trained mice were subjected to a protocol of moderate endurance exercise training over a period of 4 weeks before pregnancy. TE and CE Dams groups had access to wheels throughout pregnancy until delivery. Analyses were performed on the female offspring that did not have access to running wheels or exercise training during any portion of their lives. The relative expression levels of β-catenin, PPARƴ, OPG and RANKL were determined by Quantitative RT-PCR (qPCR).
Results: Exercise during pregnancy in isolation had no effect on any measure genes but exercise both before and during pregnancy affected all genes. Exercise only before pregnancy increased β-catenin and OPG and decreased PPARƴ, RANKL and RANKL/OPG ratio (p<0.001).
Conclusion: This study demonstrated that maternal exercise training before and during pregnancy may modulate the risk of bone disorders in offspring of mothers fed a high fat diet.
Keywords: Preconception care, Exercise, High fat diet, Offspring, Bone.
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Type of Study: Original Article |
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