Robabeh Taheripanah, Marzieh Zamaniyan, Atefeh Moridi, Anahita Taheripanah, Narges Malih,
Volume 15, Issue 6 (7-2017)
Abstract
Background: The purpose of triggering in ovulation induction is to induce the final maturation of oocytes and their release from the ovary for fertilization.
Objective: The aim of the present study was to compare the effectiveness of gonadotropin-releasing hormone (GnRH) agonist and human chorionic gonadotropin (HCG) on the final maturation of oocytes and pregnancy rates in intrauterine insemination (IUI) cycles.
Materials and Methods: In this randomized clinical trial, 110 infertile women who were selected for IUI entered the study. Ovulation induction was performed. Group I received 0.1 mg GnRH agonist as triggering and group II received 10,000 IU of HCG. The serum Estradiol (E2), Luteinizing Hormone (LH), and Follicle-Stimulating Hormone (FSH) levels were measured at 12 and 36 hr after injection.
Results: LH surge was detected in all patients. LH levels at 12 and 36 hr after triggering was higher in Group I and it washed out earlier than group II (p=0.00). The pregnancy rate was higher in Group I, but the difference was not statistically significant (26.9% vs. 20.8%, respectively p=0.46). Also, the incidence of ovarian hyperstimulation syndrome was not different between the two groups (p=0.11). There was a significant difference regarding the estradiol levels at 36 hours after triggering (p=0.00).
Conclusion: Effects of GnRH on endogenous LH surge is sufficient for oocyte releasing and final follicular maturation. Pregnancy rates and ovarian hyperstimulation syndrome incidence were not different between the groups. We suggest that GnRH agonists might be used as an alternative option instead of HCG in IUI cycles.
Heresh Moridi, Seyed Abdolhakim Hosseini, Hossein Shateri, Nejat Kheiripour, Arastoo Kaki, Mahdi Hatami, Akram Ranjbaran,
Volume 16, Issue 4 (April 2018)
Abstract
Background: Malathion is an organophosphorus pesticide that commonly used in many agricultural and non-agricultural processes. Previous studies have reported the effects of melatonin on the reproductive system. Cerium dioxide nanoparticles (CeNPs) due to their antioxidative properties are promising to impact on the development of male infertility.
Objective: The aim of this study was to evaluate the effect of CeNPs on oxidative stress and sperm parameters after malathion exposure of male rats.
Materials and Methods: 36 adult male Wistar rats were divided into 6 groups (n=6/each): Control, CeNPs -treated control (15 and 30 mg/kg/day), malathion (100 mg/ kg/day), and CeNPs -treated malathion groups (15 and 30 mg/ kg/day). At the end of the study (4 wk), the sperm counts, motility, and viability in the testis of rats were measured, also lipid peroxidation, total antioxidant capacity, and total thiol groups in homogenate testis were investigated.
Results: Malathion significantly reduced sperm count, viability, and motility than the control rats (p<0.001). Co-treatment of malathion with CeNPs 30 mg/kg had a protective effect on sperm counts (p=0.03), motility (p=0.01), and viability (p<0.001) compare to malathion group. Also, the results showed that malathion reduced testis total anti-oxidant capacity, the total thiol group, and increased testis malondialdehyde than the control rats (p<0.001). CeNPs 30 mg/kg are increased total antioxidant capacity (p<0.001) and total thiol group (p=0.03) compared to malathion group. CeNPs at both doses (15 and 30 mg/kg) improved malondialdehyde than the malathion group (p<0.001 and p=0.01 respectively).
Conclusion: CeNPs 30 mg/kg administered considerably restored testicular changes induced by malathion. The improvement of oxidative stress by CeNPs may be associated with increased sperm counts, motility and viability in the testis.
