Maryam Hormozi, Saeed Talebi, Hamid Reza Khorram Khorshid, Amir-Hassan Zarnani, Koorosh Kamali, Mahmood Jeddi-Tehrani, Haleh Soltangoraee, Mohammad Mehdi Akhondi,
Volume 13, Issue 10 (10-2015)
Abstract
Background: One of the promising methods in fertility preservation among women with cancer is cryopreservation of ovarian cortex but there are many drawbacks such as apoptosis and considerable reduction of follicular density in the transplanted ovary. One solution to reduce ischemic damage is enhancing angiogenesis after transplantation of ovarian cortex tissue.
Objective: The aim of this study was to investigate the effect of Setarud, on angiogenesis in transplanted human ovarian tissue.
Materials and Methods: In this case-control study, twenty-four nude mice were implanted subcutaneously, with human ovarian tissues, from four women. The mice were randomly divided into two groups (n=12): the experimental group was treated with Setarud, while control group received only vehicle. Each group was divided into three subgroups (n=4) based on the graft recovery days post transplantation (PT). The transplanted fragments were removed on days 2, 7 and 30 PT and the expression of Angiopoietin-1, Angiopoietin-2, and VEGF at both gene and protein levels and vascular density were studied in the grafted ovarian tissues.
Results: On the 2nd and 7th day PT, the level of Angiopoietin-1 gene expression in case group was significantly lower than that in control group, while the opposite results were obtained for Angiopoietin-2 and VEGF. These results were also confirmed at the protein level. The density of vessels in Setarud group elevated significantly on day 7 PT compared to pre-treatment state.
Conclusion: Our results showed that administration of Setarud may stimulates angiogenesis in transplanted human ovarian tissues, although further researches are needed before a clear judgment is made.
Sanaz Fathizadeh, Reza Amani, Mohammad Hossein Haghighizadeh, Razieh Hormoznejad,
Volume 14, Issue 11 (11-2016)
Abstract
Background: Premenstrual syndrome (PMS) is one of the important health problems with high incidence in young women. The exact cause of this syndrome is not clear and some theories have been declared from hormonal factors to nutritional disorders.
Objective: We investigated the correlation between serum zinc and antioxidant status with PMS.
Materials and Methods: In this case-control study, forty eight young girls were selected from a total sample of 110 students residing at university dormitories including PMS (n=23) and healthy (n=25) groups based on PMS questionnaire. Dietary intake questionnaire and blood samples were collected from all participants. Serum total antioxidant capacity (TAC) and zinc concentrations were also measured.
Results: Serum TAC and zinc concentrations were lower in PMS patients compared with healthy groups (p<0.01 and p<0.05, respectively). Healthy controls consumed lower servings of hydrogenated oils (p<0.05). There were significant differences in terms of muscle mass between the PMS and healthy groups (p<0.05). Both serum TAC and zinc levels were negatively correlated to PMS scores (r=-0.39, p<0.05 and r= -0.36; p<0.05, respectively).
Conclusion: This study shows that higher TAC and zinc serum levels are associatedwith lower risk of PMS. PMS cases have more hydrogenated oils than their normalcounterparts
