Showing 9 results for Ahangarpour
Akram Ahangarpour, Ali Akbar Oroojan,
Volume 8, Issue 4 (7-2010)
Abstract
Background: Cassia italica or Senna is a wild plant distributed in south of Iran. It increased water consumption and has a laxative effect. In traditional medicine this plant has been used for the dysmenorrheal.
Objective: In this study we investigated the effect of aqueous extract of Cassia italica on uterus contraction.
Materials and Methods: Virgin Wistar rats 200-300g were purchased. After laparatomy, a piece of uterus (1.5cm) was excised and mounted in an organ bath (10ml) containing De Jalon (29°C) and isometric contractions were recorded under 1g tension. KCl (60mM) was used to produce contractions. Cassia italica extracts after washing were added at 0.5, 1, 2 and 4 mg/ml cumulatively to the organ bath and the contractions were recorded. Uterus was separately incubated with atropine sulfate (10mM), metoclopramide (10mM) and oxytocin (10mU/ml) and the tissue spasmodic effect of the extract were recorded.
Results: Cumulative concentrations of the extract (0.5-4 mg/ml) increased the basal, peak and frequency of uterus contractions, dose-dependently (p<0.001). Incubation of the tissue with atropine sulfate and metoclopramide did not reduce the spasmodic effect of the extract. Cassia italica extract was shown the oxytocic activity on the uterine smooth muscle which most concentrations of the extract (4mg/ml) were more potent than of oxytocin (10mU/ml).
Conclusion: Cassia italica stimulated the uterus contractions without involving dopaminergic (D2), and muscarinic receptors. This extract has oxytocin mimetic effects on uterus. Since the extract has uterus contraction, therefore we suggest that more study will be necessary about abortive or contraceptive effects of this plant on pregnant uterus.
Akram Ahangarpour, Ali Akbar Oroojan, Ashraf Amirzargar, Maryam Ghanavati,
Volume 9, Issue 4 (7-2011)
Abstract
Background: Citrus aurantium is a small citrus tree, with scented white flowers. The C. aurantium is used in Asian herbal medicine primarily to treat digestive problems.
Objective: The goal of this study is to investigate the effect of C. aurantium flower's aqueous extract on uterine contraction in presence of some known uterus stimulants.
Materials and Methods: In experimental study 30 virgin Wistar rats 200-300gr were obtained. After laparatomy, a piece of Uterus was dissected out and mounted in an organ bath (10ml) containing De Jalon (29°C) and contracted by KCl (60mM), oxytocin (10mU/ml) and barium chloride (4mM) then the effect of C. aurantium flower's aqueous extract (1-8 mg/ml) on the uterine contractions was investigated. Uterus was separately incubated with propranolol (1mM), naloxone (1mM) and the role of β-adrenoceptors, opioid receptors were evaluated.
Results: Cumulative concentrations of the extract (1-8 mg/ml) decreased KCl, oxytocin and barium chloride induced uterine contractions, dose-dependently (p<0.001). C. aurantium flower's aqueous extract was unaffected on incubation the tissue with propranolol and naloxone.
Conclusion: It seems that the extract induced antispasmodic effect mainly via calcium influx blockade. However, neither β-adrenoceptors nor opioid receptors were involved. Since the extract has antispasmodic effect on uterus contraction therefore we can suggest that more study will be necessary to relief dysmenorrheal.
Akram Ahangarpour, Ali Akbar Oroojan, Maryam Radan,
Volume 12, Issue 1 (2-2014)
Abstract
Background: One of the considerable uses of lettuce (Lactuca sativa) seed in traditional medicine has been to reduce semen, sperm and sexuality.
Objective: The aim of this study was to investigate the effects of aqueous and hydro-alcoholic extracts of lettuce seed on testosterone level and spermatogenesis.
Materials and Methods: In this experimental study 24 adult male NMRI mice weighing 20-25gr were purchased. Animals were randomly divided into 4 groups: controls, hydro-alcoholic (200 mg/kg) and aqueous extracts (50, 100mg/kg). The extracts were injected intraperitoneally once a day for 10 consecutive days. 2 weeks after the last injection, the mice were anaesthetized by ether and after laparatomy blood was collected from the heart to determine testosterone by ELISA assay kit. Then testis and cauda epididymis of all animals were removed for analyzing testis morphology and sperm count and viability.
Results: Testis weight in hydro-alcoholic and aqueous extracts 100 mg/kg (p=0.001) and aqueous extract 50 mg/kg (p=0.008) groups was increased .Sperm viability in hydro-alcoholic (p=0.001) and aqueous extracts 50 (p=0.026), 100 mg/kg (p=0.045) groups was decreased, Also the results showed a significant decrease in sperm count in hydro-alcoholic (p=0.035) and aqueous extracts 50 mg/kg (p=0.006) groups in comparison with control group. Also there was a significant increase in serum level of testosterone in aqueous extract 50 mg/kg group in comparison with control (p=0.002) hydro-alcoholic (p=0.001) and aqueous extracts 100 mg/kg (p=0.003) groups.
Conclusion: Present results demonstrated that hydro-alcoholic and aqueous 50 mg/kg extracts of lettuce seed have antispermatogenic effects, also aqueous extract 50 mg/kg increased serum level of testosterone in mice. Therefore we can suggest that lettuce seed could be a potential contraceptive agent.
Akram Ahangarpour, Zohreh Lamoochi, Hadi Fathi Moghaddam, Seyed Mohamad Taghi Mansouri,
Volume 14, Issue 3 (3-2016)
Abstract
Background: Aging contains morphological and functional deterioration in biological systems. D-galactose (D-gal) generates free radicals and accelerates aging. Portulaca oleracea (Purslane) may have protective effect against oxidative stress.
Objective: Purslane ethanolic extract effects were evaluated on antioxidant indices and sex hormone in D-gal aging female mice.
Materials and Methods: 48 female NMRI mice (25-35 gr) were randomly divided into, 6 groups: 1- control (normal saline for 45 days), 2- Purslane (200 mg/kg for last 3 weeks), 3-D-gal (500 mg/kg for 45 days), 4-D-gal+Purslane, 5- Aging, 6-Aging+Purslane. Sex hormones, antioxidants and malondialdehyde (MDA) level of ovary and uterus were measured. Histological assessment was also done.
Results: In D-gal treated and aging animals, LH and FSH levels were significantly increased (p<0.001) while estrogen and progesterone levels were significantly reduced (p<0.001) in comparison with control group. MDA contents were significantly increased in ovaries and uterus of D-gal and aging groups (p<0.01). Superoxide dismutase (SOD) (p<0.001) and catalase (p<0.01) activities were significantly decreased in both aging and D-gal treated animals. Ovarian follicles were degenerated and atrophy on uterine wall and endometrial glands was observed in D-gal and aging groups. Alteration in hormone levels, MDA contents and antioxidant activity were significantly reversed by Purslane (p<0.05). Purslane could also improve histological changes such as atrophy of endometrium.
Conclusion: These findings indicate that Purslane can attenuate aging alternations induced by D-gal and aging in female reproductive system.
Mina Omidi, Akram Ahangarpour , Seyed Ali Mard, Layasadat Khorsandi,
Volume 17, Issue 11 (November 2019)
Abstract
Abstract
Background: Aging is accompanied by decreasing general function in the cells and tissues. D-galactose (D-gal) induces aging and plays a role in the pathogenesis of it. Myricitrin is a plant-derived antioxidant.
Objective: The present study was performed to evaluate the effects of myricitrin on antioxidant defense, sex hormone levels, uterus, and ovarian histology in D-gal-induced aging female mouse model.
Materials and Methods: In this experimental study, 72 female adult NMRI mice, weighing 30-35 gr, 3-4 months old, were randomly divided into six groups (n = 12/each): (I) Control (vehicle; normal saline), (II) D-gal at 500 mg/kg/d for 45 days, (III-V) D-gal + myricitrin-treated groups (these groups received myricitrin at 5, 10, and 20 mg/kg/d, and (VI) D-gal + 100 mg/kg/d vitamin E orally for the last 28 days. The antioxidant indices were done on the basis of colorimetric method, and sex hormone levels were measured by using enzyme-linked immunosorbent assay kits. Histological assessment of the uterus and ovaries were also evaluated.
Results: D-gal impaired the estrous cycle, also degenerative changes occur in the ovarian follicles and damage to the uterus and ovarian tissue occurs. In D-gal group, the level of sex hormones (p =0.03) and the total antioxidant capacity (p = 0.002) decreased, while the level of malondialdehyde and gonadotropins increased (p = 0.03). Myricitrin at lower doses and vitamin E ameliorated the D-gal effects.
Conclusion: These findings suggest that myricitrin at low doses can effectively prevent D-gal-induced oxidation and aging in mice. The effect of myricitrin was equivalent and sometimes better than vitamin E.
Kheirollahi M, Ahangarpour A, Khorsandi L,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Diabetes has recently been a serious problem in the world. Sexual and reproductive disorders are one of the most important secondary complications in patients with diabetes.
Objective: The effect of crocin on methylglyoxal (MGO)-induced diabetes in the male reproductive system has not been studied yet; so this study performed on MGO-induced diabetic male mice.
Materials and Methods: 70 male NMRI mice, one-month-old, weighing 20-25 g were divided into 7 groups (n = 10): sham, MGO (600 mg/Kg/d), MGO+crocin15, 30 and 60 mg/kg/d, MGO+Metformin (200 mg/kg/d), and crocin 60 mg/kg/d. Methylglyoxal administered orally in 30 days. In 14st day, after proving hyperglycemia, Metformin and crocin administered orally. On the 31st day of the study, plasma and tissue samples prepared for experimental assessments.
Results: Blood glucose and insulin levels in the MGO group are higher than the sham group (p < 0.001), and decreased with Metformin (p < 0.001) and crocin treatment (not in all doses). Testis width and volume decreased in the MGO receiving mice, and improved in crocin treated mice (p < 0.05), but not in the metformin group. Superoxide dismutase decreased in diabetic mice (p < 0.05) and Malondialdehyde enhanced (p < 0.001). Crocin and Metformin improved MDA and SOD. Testosterone (p < 0.001), and sperm count (p < 0.05) decreased in diabetic mice, treatment in all doses recovered these variables. Luteinizing hormone increased in diabetic mice (p < 0.001) and crocin treatment (but not metformin) decreased it. Seminiferous diameter and height decreased in diabetic mice and increased in treatment groups. Vacuoles and ruptures have been seen in diabetic testicular tissue, crocin improved testicular morphology (p < 0.01).
Conclusion: MGO increases oxidative stress, reduces sex hormones, and induces histological problems in male reproductive organ. Crocin and metformin improved the reproductive damage caused by MGO induced diabetes.
Zeinab Behdarvand-Margha, Akram Ahangarpour, Mohammadreza Shahraki, Gholamreza Komeili, Layasadat Khorsandi,
Volume 19, Issue 8 (August 2021)
Abstract
Background: Diabetes mellitus is a disease that has reached a dangerous point. Today, nearly 500 million men and women around the world live with diabetes. Gallic acid (Gal) affects diabetes.
Objective: To evaluate the effects of Gal and metformin (met) on the levels of glucose, insulin, testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), sperm count, antioxidant status, and histological changes in the testes of diabetic mice induced by methylglyoxal (MGO).
Materials and Methods: In this experimental study, 50 male adult NMRI mice, weighting 25-30 gr, aged 3-4 months were randomly divided into five equal groups (n = 10/each). (i) Control (vehicle, normal saline), (ii) MGO (600 mg/kg/d) orally for 28 days, (iii) Gal (50 mg/kg/d), (iv) MGO+Gal, and (v) MGO+met (200 mg/kg/d). Gal and met were administered orally for 21 consecutive days after the induction of diabetes. Blood samples were taken at 24 hr after the latest doses of treatment. Histological assessment of the testis was done, and the epididymis sperm count was obtained. Antioxidant indices, glucose, insulin, LH, FSH, and testosterone levels were measured.
Results: In the MGO group compared to the control group, insulin, glucose (p = 0.001), LH (p = 0.04) and malondialdehyde (p = 0.001) were increased. However, the level of testosterone (p = 0.001), seminiferous tubule diameters, epithelial height, sperm count, superoxide dismutase activity (p = 0.02), and testis volume (p = 0.01) were decreased. The results indicated that Gal and met ameliorated the MGO effects.
Conclusion: These findings suggested that the animals receiving MGO became diabetic. According to the results, Gal and met can effectively prevent MGO-induced diabetes. The effect of Gal was equivalent and sometimes better than metformin.
Seyed Sobhan Bahreiny, Akram Ahangarpour, Ali Asghar Hemmati, Razieh Kazemzadeh, Mohammad-Navid Bastani, Mohammad Reza Dabbagh, Mojtaba Aghaei,
Volume 21, Issue 10 (October 2023)
Abstract
Background: Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder in females. Nesfatin-1 is a neuropeptide synthesized in the hypothalamus and other peripheral organs, and there are conflicting opinions about its correlation with PCOS.
Objective: This study aims to investigate the correlation between nesfatin-1 and PCOS and evaluates the effectiveness of nesfatin-1 as a biomarker for the detection of PCOS in women.
Materials and Methods: A systematic review and meta-analysis were conducted to identify pertinent articles from databases such as PubMed, Web of Science, Cochrane, EMBASE, Scopus, and Google Scholar. The standardized mean difference (SMD) and 95% confidence interval (CI) were calculated using a random effects model to compare group outcomes. Additionally, meta-regression and subgroup analysis were performed to elucidate sources of heterogeneity.
Results: The meta-analysis involved 12 studies with 1222 participants, and the findings revealed a significant relationship between PCOS and nesfatin-1 levels. The pooled (SMD = 0.54; 95% CI: 0.00-1.07; p = 0.04) indicated a significant difference between the evaluated groups. Moreover, a subgroup analysis showed that there was a substantial difference in nesfatin-1 levels among women with PCOS and higher homeostatic model assessment for insulin resistance ratio (SMD = 1.46; 95% CI: 0.92-2.00; p < 0.001).
Conclusion: Our meta-analysis indicates an association between high nesfatin-1 levels and PCOS. This suggests a potential role of nesfatin-1 in the development of PCOS and proposes it as a potential diagnostic biomarker for the disease. However, further research is necessary to validate these findings.
Seyed Sobhan Bahreiny, Akram Ahangarpour, Elnaz Harooni, Mahdi Amraei, Mojtaba Aghaei, Reza Mohammadpour Fard,
Volume 22, Issue 12 (December 2024)
Abstract
Background: Polycystic ovary syndrome (PCOS) casts a wide shadow over the reproductive health of millions of women worldwide, emerging as one of the most complex and multifaceted endocrine disorders. In addition, nitric oxide (NO) stands out as a pivotal signaling molecule, orchestrating a symphony of physiological processes.
Objective: This meta-analysis aims to elucidate the association between NO levels and PCOS, investigate the potential of NO as a biomarker for PCOS diagnosis, and evaluate its clinical significance.
Materials and Methods: A systematic review was conducted in several electronic databases, including PubMed, Web of Science, Cochrane Library, Scopus, EMBASE, and Google Scholar, to identify relevant studies published up to January 2024. Standardized mean difference and 95% CI were calculated using a random effects model to assess the overall effect size. Meta-regressions and subgroup analysis were performed to investigate sources of heterogeneity.
Results: A meta-analysis of 14 studies with 1171 participants showed that NO levels were significantly lower in the PCOS group than in the control group. The pooled analysis yielded a standardized mean difference of -0.482; 95% CI: -0.908 to -0.056; p = 0.027. Subgroup analyses further demonstrated variations in NO levels between different PCOS phenotypes and in relation to metabolic parameters.
Conclusion: This meta-analysis provides evidence for an association between PCOS and dysregulated NO levels and suggests a potential role of NO as a biomarker in the diagnosis and pathogenesis of PCOS.
Registration ID in PROSPERO: CRD42024507048
