Showing 4 results for Amiri S
Ghasemi-Esmailabad S, Talebi Ah, Talebi Ar, Amiri S, Moshrefi M, Pourentezari M,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Morphine is one of the major psychoactive chemicals in opium that can increase the production of free radicals and thus can negatively affect spermatogenesis.
Objective: The purpose of this survey was to demonstrate the effect of morphine consumption on sperm parameters, DNA integrity and apoptosis in men taking morphine.
Materials and Methods: In this case-control study, 30 man abusing morphine (cases) and 30 healthy men (controls) were compared for sperm parameters (count, motility and morphology) and sperm chromatin quality, with aniline blue, toluidine blue and Chromomycin A3 stainings. The participants were matched for age, weight, amount and duration of cigarette smoking.
Results: In men with morphine dependency, sperm progressive and total motility (p = 0.038 and p < 0.001 respectively) showed significant decreasing compared to control group. Regard to morphine abusing, although morphine can decrease the sperm chromatin condensation and increases the rate of sperm apoptosis, but these differences were not statistically significant.
Conclusion: According to our result morphine dependence can reduce male fertility by affecting sperm parameters and also it may affect sperm chromatin/DNA integrity.
Hojati S, Miresmaeili Sm, Montazeri F, Amiri S, Hoseini Sm, Kalantar Sm, Fesahat F,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Thin long tail deletion of Y chromosome is the most common molecular genetic cause of infertility. It is considered to be severe in men which occurs in the three region of the azoospermic factor; AZFa, AZFb and AZFc. These region contain multiple genes involved in spermatogenesis.
Objective: The aim of this study was to investigate the Y chromosome deletion pattern among infertile men with globozoospermic referring to Yazd Infertility Treatment Center.
Materials and Methods: 19 infertile men referred to Yazd Reproductive Science Institute with globozoospermia (from 2014 to 2016) were studied considering microdeletions in Y chromosome. Using multiplex PCR and six different STS (Sequence-Tagged Site) markers microdeletions of Y chromosome in AZFa, AZFb and AZFc regions was analysed.
Results: In our samples, the deletion of AZF regions of the Y chromosomes was not abserved in any blood sample of globozoospermic man.
Conclusion: In 19 samples, no defect was observed in the AZF regions of the Y chromosomes was not the cause of globozoospermia.
Amiri S, Amjadi F, Aflatoonian R, Ashrafi M, Akbari Sene A, Mehdizadeh M, Zandieh Z,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Although embryo selection for transfer is usually based on morphology, 70% of embryos with high morphological quality have chromosomal abnormalities. The results of implantation and pregnancy rate assessments following preimplantation genetic screening (PGS) are controversial. There is still no in vitro study to compare the implantation of human euploid and aneuploid embryos.
Objective: This study was designed to compare the ability of aneuploid embryos to attach to endometrial cells with euploid embryos by simulating the human endometrium using a three-dimensional scaffold.
Materials and Methods: After informed consent, 10 endometrial biopsies were taken from fertile women. Endometrial cells were isolated and expanded in 2D cultures to achieve enough cells. The fibrin-agarose scaffold was made and stromal cells were cultured into the scaffold, after 24 hr, the epithelial cells were seeded on the scaffold. Cell culture continued for 5 days to reach the appropriate confluence. Then, cell proliferation was assessed by MTT assay. The simulated endometrial construct was confirmed by H&E and immunohistochemistry (IHC). The embryos were also examined by performing PGS following conventional comparative genomic hybridization array. 10 euploid and 10 aneuploid blastocysts were selected for co-culturing. Partial hatching of blastocysts was performed using a laser system. Blastocysts were co-cultured with the 3D structure of human endometrial cells for 72 hr. The blastocyst's attachment to the endometrial-like structure was examined under a phase-contrast microscope and scanning electron microscopy.
Results: The MTT OD of scaffolds increased during 5 days of cell culture (p < 0.05). The histological evaluation of the co-culture systems was done under light microscopy by H&E staining. On the top of the 3D culture system, epithelial cells shaped a constricted cell monolayer. Stromal cells combined with the fibrin-agarose scaffold got lengthened and expanded, displaying that the 3D culture systems supplied a suitable environment for the growth of endometrial cells. In the 3D culture, the origins and locations of epithelial and stromal cells were defined by cytokeratin and vimentin immunostaining, respectively. IHC for cytokeratin was only positive for epithelial cells in the surface epithelium. IHC for the vimentin was positive for the stromal cells located in the 3D matrix. These results showed that fibrin-agarose scaffold could simulate the human endometrial structure. Using scanning electron microscopy and phase-contrast microscopy, it was found that only euploid embryos were able to attach to the endometrial construct while aneuploid embryos weren't.
Conclusion: Our findings determined that PGS allows us to transfer top-quality embryos with higher implantation potential. It improves implantation and pregnancy rate during assisted reproductive technologies cycles, especially in patients with recurrent implantation failure.
Mosavi A, Amjadi F, Barati M, Aflatoonian R, Amiri S, Mehdizadeh M, Mirsanei J,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Preimplantation genetic diagnosis (PGD) is a useful clinical tool to identify embryos with or at risk of specific genetic malady before embryo implantation. Current procedures for embryo chromosomal screening require an invasive biopsy of the embryo. Blastomere biopsy has a potential lesion to the embryos may result in developmental defects or abortion. Thus, a non- invasive PGD is needed.This study hypothesized that embryonic DNA is present in the spent culture medium. We focused on X-linked disorders, these single-gene diseases due to the presence of defective genes on the X chromosome are dominant in males.
Objective: Therefore, the objective of this study was to discriminate between female (XX) and male (XY) embryos by detecting Y chromosome- specific genes in cell-free DNA and comparing to PGD results. It opens a new window for the development of a non-invasive PGD method.
Materials and Methods: Embryo´s spent media from day 3 and day 5 embryos development were collected. The modified phenol-chloroform solution was used for DNA extraction from spent media. DNA from spent media was evaluated using SRY, TSPY, and AMELOGENIN as targets using the qPCR method. IBM SPSS and Medcals were used for statistical analyses, to compare sex determination of embryos using spent medium with PGD results.
Results: Yield and purity of the extracted DNA as well as repeatability of the method were performed well using the modified phenol-chloroform solution. The amount of DNA at day 5 embryo culture medium was significantly higher than day 3. Results of sex determination using spent medium by Q-PCR were consistent with the results of PGD and 12th wk sonography. This invasive PGD method using a spent culture medium gave a sensitivity of 66.7%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 67.6 (N = 56, Nxx = 23, Nxy = 33).
Conclusion: This investigation provides a potentially effective procedure that can help to avoid the invasive preimplantation genetic diagnosis, especially about X-linked diseases. Results of sex determination using spent medium by Q-PCR were consistent with the results of PGD. Improvements in DNA collection, amplification, and testing may allow for PGD without biopsy in the Future.
