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Showing 3 results for Asemi-Rad
P-30 Upregulation of elafin expression in the fallopian tube of ectopic tubal pregnancies compared to the normal tubes
Zakizadeh F, Mahmoudzadeh-Sagheb H, Asemi-Rad A, Ghasemi M, Moudi B, Sheibak N, Asadikalameh Z, Heidari Z,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Ectopic pregnancy is one of the most important causes of maternal mortality and fallopian tubes are the location of 95% of ectopic pregnancies. Elafin is a natural antimicrobial molecule that plays an important role as an anti-inflammatory agent in mucosal surfaces and has been found in the female reproductive tract.
Objective: The aim of this study was to investigate elafin expression, in the fallopian tube mucosa of ectopic pregnancies compared to the normal tubes using immunohistochemistry techniques and quantitative reverse transcription (qRT-PCR).
Materials and Methods: In this case-control study, uterine tube samples were obtained from patients with an indication for surgical removal of the tubes. The case group (n = 20) consisted of patients who were undergoing salpingectomy due to an ectopic pregnancy, the control group (n = 20) included patients who had a salpingectomy and hysterectomy. Using qRT-PCR and immunohistochemistry, the expression of elafin was investigated in both study groups.
Results: Immunohistochemical expression of elafin in the epithelium and connective tissue was significantly increased in the implantation site of the patients in comparison with the control group (p < 0.001). The level of elafin mRNA increased in the mucous membrane of the fallopian tube from patients with the ectopic pregnancy compared to the normal mucosa (p < 0.001).
Conclusion: Increasing expression of elafin during an ectopic pregnancy may be a mechanism for enhancing innate immune response and be involved in related pathological conditions such as infection and ectopic implantation.

P-81 Cannabinoid receptor type-1 (CB1) and its correlation with CB1 gene polymorphism-1359G/A in ectopic pregnancy compared to the control group
Moudi B, Heidari Z, Asemi-Rad A, Mahmoudzadeh-Sagheb H, Sheibak N, Ghasemi M, Eslami S,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Ectopic pregnancy (EP) is one of the most important causes of maternal mortality. Novel information could help to identify the signaling pathway and mechanism of fetal transfer disruption and its potential use in the diagnosis and prevention of this disorder.
Objective: This study aimed to evaluate the immunohistochemical expression of the cannabinoid receptor type 1 (CB1) and its association with CB1 -1359G/A gene polymorphism (rs1049353) in the fallopian tubes in EP compared to controls.
Materials and Methods: In this case control study, 100 women with EP (cases) and 100 women that underwent abdominal surgery due to the hysterectomy or uterine tubal ligation (healthy controls) were included. Genotyping of CB1-1359G/A polymorphism, tissue expression of CB1 at the protein and mRNA levels were studied using restriction fragment polymorphism, immunohistochemical (IHC) method, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis.
Results: Genotyping showed that in EP, the frequency of AA, AA+AG genotypes, and A allele was significantly higher than healthy control subjects (p = 0.001). Also, patients with EP had significantly increased IHC expression of CB-1 compared to the control samples (p = 0.016). Patients with AA and AG genotypes had a significantly higher IHC expression of CB-1 compared to the GG genotype. Quantitative real-time PCR analysis showed that patients with EP had significantly increased expression of CB-1 compared to the control samples (p < 0.001). Patients with AA and AG genotypes had higher significant mRNA expression of CB-1 compared to the GG genotype.
Conclusion: Based on molecular and cellular analysis of CB1, the frequency of an allele and expression of CB1 were higher in patients with ectopic tubal pregnancy. Identifying the causes of the EP is essential to find effective methods of prevention and treatment of EP. CB1 is likely to be effective in creating innate immunity in humans and can affect the process of EP in the fallopian tube. CB1 is also a pathological valuable factor in identifying the pathway of inflammation during ectopic implantation. However, it is not possible to claim that a single SNP could be the only cause of the EP. In other words, EP is a polygenic disease, but the possible effects of these genetic changes in EP remain unknown. More investigations are required to introduce a risk prediction tool for susceptibility to EP.

Prevalence of congenital anomalies and related factors in live births in Zahedan, Southeast of Iran: A cross-sectional study
Azam Asemi-Rad, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb, Yousef Mehdipour, Bita Moudi, Nadia Sheibak, Saeid Ebrahimi,
Volume 21, Issue 8 (August 2023)
Abstract
Background: The term congenital anomalies (CAs) refer to structural or functional abnormalities at the time of conception. Approximately 12 deaths related to congenital disabilities occur in every 10,000 babies born.
Objective: This study aimed to evaluate the prevalence and associated factors of single and multiple CAs in live births in Zahedan, Southeast Iran.
Materials and Methods: This cross-sectional study was conducted on 59,087 live births in a referral hospital in Zahedan located in the southeast of Iran during 2009-2019. All live births were examined by pediatricians and the CAs and categorized based on the international classification of diseases.
Results: Of 59,085 live births, at least 883 had a significant anomaly, and the prevalence rate of CAs was about 149 per 10,000. Anomalies of the nervous (24.1%) and cardiovascular systems (21.10%) were the most frequent, occurring in 213 and 187 of the live births, respectively. Spina bifida is the most common anomaly of the central nervous system. The most common anomalies in the cardiovascular system were unspecified heart malformations (17.1%), cardiovascular malformations (18.7%), and patent ductus arteriosus (11.7%). Significant correlations were found between the parent's consanguinity marriage, the mother's age, an existing anomaly in the family, and relatives in single and multiple CAs (p = 0.02, p = 0.02, p < 0.001, p = 0.01, respectively).
Conclusion: The prevalence of CAs was 149 per 10,000 live births. The highest prevalence of CAs was related to the central nervous system. Increasing the public's knowledge about fetal defects can reduce the prevalence of CAs.

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