Showing 7 results for Khaki
Arash Khaki, Mahnaz Heidari, Marefat Ghaffari Novin, Amir Afshin Khaki,
Volume 6, Issue 3 (7-2008)
Abstract
Background: Ciprofloxacin is a commonly prescribed antibiotic in the treatment of genitourinary tract infection.
Objective: The aim of this study was to investigate the effects of ciprofloxacin on testis apoptosis and sperm parameters in rat.
Materials and Methods: Twenty male Wistar rats were selected and randomly divided into two groups; control (n=10) and experimental (n=10). The experimental group was orally received 12.5 mg/kg ciprofloxacin daily for 60 days and the control group just received water and food. Rats were then killed and sperm removed from cauda epididymis and analyzed for sperm motility, morphology, and viability. Testis tissues were also removed and prepared for TUNEL assay to detect apoptosis.
Results: Results showed that ciprofloxacin significantly decreased the sperm concentration, motility (p<0.05) and viability (p<0.001). In addition, ciprofloxacin treatment resulted in a significant decrease in the number of spermatogenic cells (spermatogonia, spermatocyte, spermatid and sperm) in the seminiferous tubules when compared with the control group. The apoptotic germ cells per seminiferous tubular cross section was significantly increased in the experimental group (15.11±3.523) as compared with the control group (7.3±0.762) (p<0.05).
Conclusion: It is concluded that ciprofloxacin has the toxicological effects on reproductive system in male rats.
Amir Afshin Khaki, Arash Khaki, Shahram Garachurlou, Fereshteh Khorshidi, Nazila Tajadini, Navid Madinei,
Volume 6, Issue 3 (7-2008)
Abstract
Background: Men are unavoidably exposed to ambient electromagnetic fields (EMF) generated from various electrical gadgets and from power transmission lines. Prostate gland plays an important role in secretion of semen as largest accessory gland of male reproductive system. It seems that protection of this gland against EMF is important in spermatogenesis process.
Objective: The goal of this study was to investigate the effect of non ionizing radiation on ultra structure of prostate gland.
Materials and Methods: In total 50 male and 50 female rats, aged 15 weeks, were mated in animal house of Tabriz University of Medical Sciences. Then among born rats, 20 randomly were chosen as control and 30 were randomly chosen for exposure to EMF. They were exposed to 50 Hz EMF (8 M.T.) during in utero development (approximately 3 weeks) and postnatal life (5 weeks). Samples of prostate gland were processed and observed under light and transmission electron microscope.
Results: In the experimental group, the secretory epithelial cells were generally inactive and cuboidal and their nuclei were dense with more corpus amylace compared to the control. Smooth muscle fibers spread out in different directions with heterochromatic nuclei. Mitochondria seemed without cristae and electron opaque.
Conclusion: The results indicate that EMF had a deleterious effect on ultra structure of prostate gland in rat.
Arash Khaki, Fatemeh Fathiazad, Mohammad Nouri, Amir Afshin Khaki, Chelar C Ozanci, Marefat Ghafari-Novin, Mohammad Hamadeh,
Volume 7, Issue 1 (7-2009)
Abstract
Background: Ginger rhizome (Zingiber officinale R., family: Zingiberaceae) is used medicinally and as a culinary spice.
Objective: Medicinal use of ginger dates back to ancient China and India. Ginger and its constituents are stated to have antiemetic, antithrombotic, antihepatotoxic, anti-inflammatory, stimulant, cholagogue and antioxidant. It has been used since ancient time as medicinal and food origins it contain antioxidative and androgenic activities and have well effect in diseases treatment in more countries world-wide. As an antioxidant’s ginger has a useful effect on spermatogenesis and sperm parameters.
Materials and Methods: Wistar male rat (n=30) were allocated into three groups, control (n=10) and test groups (n=20), that subdivided into groups of 2 that received ginger rhizome powder (50 and 100mg/kg/day) for 20 consequence day. Animals were kept in standard conditions. In twentieth day the testes tissue of Rats in whole groups were removed and sperm was collected from epididymis and prepared for analysis.
Results: Serum total testosterones significantly increased in experimental group that has received 100 mg/kg/day Ginger (p<0.05) in comparison to control group. Besides, the percentage of sperm viability and motility in both test groups significantly increased (p<0.05) in comparison to control group, Whereas, LH, FSH hormones, sperm concentration, morphology and testes weights in both experimental and control group were similar.
Conclusion: Results revealed that administration of 100 mg/kg/day of ginger significantly increased sperm percentage, viability, motility and serum total testosterones. This suggested that ginger may be promising in enhancing sperm healthy parameters.
Arash Khaki,
Volume 13, Issue 3 (3-2015)
Abstract
Background: Antibiotic therapies used in treatment of many diseases have adverse effects on fertility. This review analyzes previous comparative studies that surveyed the effects of two common groups of antibiotics on male fertility.
Objective: To evaluate histo-pathological effects of fluoroquinolones and aminoglycosides on sperm parameters and male reproductive tissue.
Materials and Methods: Articles about the effects of aminoglycosides and fluoroquinolones on male infertility, sperm parameters, male reproductive tissue, and spermatogenesis in English and Persian languages published on Google Scholar and PubMed databases from January 2000 to December 2013 were assessed. Randomized controlled trials (RCTs) assessing the effects of aminoglycosides or fluoroquinolones on sperm parameters, artificial insemination, and male reproductive tract or RCTs comparing aminoglycosides vs. fluoroquinolones were eligible for inclusion. For ascertaining the reliability of study, data were extracted independently and in duplicate by two investigators.
Results: Sperm viability was decreased significantly with streptomycin, gentamicin, and neomycin (p<0.001). Sperm motility was decreased significantly with gentamicin and neomycin (p<0.05). Total sperm count was significantly decreased with ofloxacin, gentamicin, streptomycin, and neomycin (p<0.022). There was significant decrease in post-thawing motility with low dose and high dose of ciprofloxacin. Testis weight was decreased with gentamicin and ofloxacin significantly (p<0.011). There was significant decrease in seminal vesicle weight with gentamicin, neomycin, and ofloxacin (p<0.022). Furthermore, changes in epididymis weight, percentage of total apoptotic cells, and diameter of seminiferous tubule were significant with all drugs including streptomycin, gentamicin, neomycin, and ofloxacin (p<0.05).
Conclusion: Streptomycin has less negative effects on cell’s apoptosis and sperm parameters as compared to other drugs. Gentamicin has more detrimental effects so lesser dosage and duration is recommended. Fluoroquinolones showed negative effects on testis tissue and sperm parameters. Ciprofloxacin has less adverse effects than gentamicin in artificial insemination.
Amir Afshin Khaki, Arash Khaki, Seyed Shahin Ahmadi,
Volume 14, Issue 2 (2-2016)
Abstract
Background: Recently, there are increasing concerns and interests about the potential effects of Electromagnetic Field (EMF) on both human and animal health.
Objective: The goal of this study was to evaluate the harmful effects of 50 Hz non-ionizing EMF on rat oocytes.
Materials and Methods: In this experimental study 30 rats were randomly taken from laboratory animals and their ags and weights were determined. These 3 month's old rats were randomly divided into 3 groups. The control group consisted of 10 rats without receiving any treatment and kept under normal conditions. Experimental group 1 (10 rats) received EMF for 8 weeks (3 weeks intrauterine +5 weeks after births) and experimental group 2 (10 rats) received EMF for 13 weeks (3 weeks intrauterine +10 weeks after birth). After removing the ovaries and isolating follicles, granulosa cells were fixed in glutaraldehyde and osmium tetroxide. Electron microscopy was used to investigate the traumatic effects of EMF on follicles.
Results: In control group nucleus membrane and mitochondria in follicle’s cytoplasm seemed normal in appearance. Theca layer of primary follicles in experimental group was separated clearly, zona layer demonstrated trot with irregular thickness and ovarian stroma seemed isolated with dilated vessels showing infiltration.
Conclusion: According to the results of this study, it can be concluded that EMF has harmful effects on the ovarian follicles
Mohammadreza Gholami, Seyyed Amir Yasin Ahmadi, Abolfazl Abaszadeh, Arash Khaki,
Volume 15, Issue 5 (6-2017)
Abstract
Spermatocytogenesis starts from lumens of seminiferous cords and after migrationto the basal membrane ends to the lumens again. We attempt to review the protectiveeffects of melatonin and ghrelin on Spermatocytogenesis and in particular onspermatogonial stem cells, as two rather newly-discovered hormones. Testicularfreezing prior to chemotherapy and radiotherapy is one of the ways of preservingfertility in children with cancer. The freezing has two methods of slow-freezing(cryopreservation) and rapid-freezing (vitrification). Administration of melatonincan maintain the quality of the germ cells underwent such processes, as well asghrelin, can protect germ cells from the toxicities secondary to ischemic injuries,and pathologic apoptosis. This review indicates that in vitro or in vivoadministration of melatonin or ghrelin, could be effective to preserve fertilizationand also they can be used in assisted reproductive technologies to improve thequality of sperms. Future original studies should be propelled toward human studies,of course with observing the ethics.
Ramesh Baradaran Bagheri, Mahshid Bazrafkan, Abbas Sabour, Mina Ataei, Bita Badehnoosh, Banafsheh Mashak, Bahareh Khakifirooz, Ramin Moghaddam,
Volume 21, Issue 7 (July 2023)
Abstract
Background: The benefits of frozen embryo transfer (FET) vs. fresh embryo transfer for in vitro fertilization (IVF) have been discussed in previous studies.
Objective: To determine and compare the pregnancy outcomes following FET and frozen embryos transfer in women who underwent assisted reproductive techniques.
Materials and Methods: In this cross-sectional study, 233 women candidates for IVF/intra cytoplasmic sperm injection who referred to the Kamali Training Medical Center, Karaj, Iran during 2019-2020 were evaluated in 2 groups of fresh (n = 127) and frozen (n = 106) embryo transfers. The rates of pregnancy outcomes including chemical and clinical pregnancy, live birth, preeclampsia, ectopic pregnancy, still birth, and pregnancy loss were compared between groups in 3 age subgroups (< 25, 25-35, and 35-40 yr old).
Results: No significant difference in terms of chemical and clinical pregnancy and live birth rates were observed between groups in women aged < 25 yr. Chemical and clinical pregnancy and live birth rates were significantly higher in the FET group compared to fresh group in 25-35-yr-old women (p = 0.01, p = 0.03, and p = 0.01, respectively). In 35-40-yr-old women, no significant differences were observed in terms of chemical and clinical pregnancy rates, but live birth rate was found to be significantly higher in the FET group (p = 0.02). The pregnancy loss was lower in the FET group (p = 0.038).
Conclusion: In conclusion, the FET method in women aged 25-35 yr significantly increases the chance of successful IVF/intra cytoplasmic sperm injection.
