Showing 27 results for Montazeri
Seddigheh Abdollahi Fard, Parvin Mostafa Gharabaghi, Farnaz Montazeri, Omid Mashrabi,
Volume 10, Issue 4 (8-2012)
Abstract
Background: Hysteroscopy is a safe and high efficient procedure so it is changing to a widespread procedure in dealing with many gynecologic and obstetrical conditions.
Objective: This study aimed to evaluate the diagnostic and therapeutical efficiency of hysteroscopy in managing the common conditions including abnormal uterine bleeding, abortion and infertility.
Materials and Methods: This was a descriptive cross-sectional study to compare hysteroscopy as a minimally invasive approach with conventional laparatomy and hysterectomy or repair of mulerian anomalies and watch the uterine cavity for intrauterine pathology in cases of infertility. Overall 277 women underwent hysteroscopy were evaluated in three groups: with AUB 226 cases, with infertility 34 cases and with recurrent abortions with septate uterus17 cases. The overall success rate was recorded and analyzed after six months in order of indication of hysteroscopy
Results: Hysteroscopy as sole diagnostic procedure in 16.5, 8.8 and 14.3%of AUB, infertility and abortion cases, respectively. In AUB cases, curettage, myomectomy, polypectomy and hysterectomy were the main diagnostic-therapeutical approaches along with hysteroscopy. In infertiles, myomectomy, polypectomy were the main diagnostic-therapeutical approaches
In abortion group, laparoscopy guided, septum resection adhessiolysis , curettage and myomectomy were the main aproach. There was not any major complication. The diagnostic-therapeutically measures accompanying with the hysteroscopy were successful in 73.5% of the bleeding group and 33.3% of the infertility group in follow-up period.
Conclusion: Based on our results, hysteroscopy is a safe, accurate and highly-efficient procedure in managing women with abnormal uterine bleeding, recurrent abortion due to septate uterus
Fatemeh Bazarganipour, Saeide Ziaei, Ali Montazeri, Fatemeh Foroozanfard, Soghrat Faghihzadeh,
Volume 11, Issue 5 (7-2013)
Abstract
Background: Polycystic ovarian syndrome (PCOS) has been shown to cause a reduction in Health-related quality of life (HRQOL).
Objective: This study examines the extent of different clinical symptoms in PCOS patients on HRQOL.
Materials and Methods: A cross-sectional study was undertaken to ascertain the factors related to HRQOL in 200 PCOS patients in Kashan, Iran. Main outcome measures were modified polycystic ovarian syndrome questionnaire (MPCOSQ) and clinical information of PCOS. Major clinical PCOS features including obesity (BMI), excessive body hair (hirsutism score), acne, menstrual cycle disturbances and infertility.
Results: Findings showed that the most common HRQOL concern was menstrual irregularities and infertility, followed in descending order by hirsutism, weight, emotion, and acne. Multivariate analysis revealed the menstrual irregularities as a significant predictor of menstruation (p=0.005), emotion (p=0.02) and infertility (p=0.02) subscales of the MPCOSQ. Having of infertility, predicted scores on the infertility subscale (p<0.0001). Hirsutism score was a significant predictor of hirsutism (p<0.0001) and emotion (p<0.0001) subscales. Weight subscale concerns was predicted by BMI (p<0.0001), also, acne was found to be predicted score of acne subscale (p<0.0001).
Conclusion: Worsened HRQOL in women with PCOS was related to more menstrual irregularities and infertility than to obesity. The finding suggests a potential for poorer compliance with weight management protocols among affected PCOS patients.
Fatemeh Bazarganipour, Saeide Ziaei, Ali Montazeri, Fatemeh Foroozanfard, Soghrat Faghihzadeh,
Volume 11, Issue 9 (12-2013)
Abstract
Background: A preliminary report indicated that the Iranian version of modified polycystic ovary syndrome health-related quality of life questionnaire (MPCOSQ) is a valid measure of health-related quality of life (HRQOL) in PCOS patients. Accordingly, the Iranian version of MPCOSQ was subjected to further psychometric analyses among a different sample of patients with PCOS.
Objective: To examine discriminant and convergent validity of the Iranian version of MPCOSQ.
Materials and Methods: This was a cross sectional study of 200 women with PCOS that was carried out in two private gynecology clinics in Kashan, Iran. Discriminant validity was assessed using the known groups comparison. Convergent validity was evaluated by assessing the correlation between similar content on the MPCOSQ and the SF-36.
Results: The mean scores for the MPCOSQ showed that women rated lowest on the infertility and menstrual subscales indicating worst health in these dimensions. The results from the SF-36 questionnaire indicated that emotional and vitality domains were the areas of poorest health. Known groups comparison showed that the MPCOSQ differentiated well between sub-groups of women who differed in PCOS specific symptoms, lending support to its discriminant validity. Convergent validity was assessed and as expected a good positive correlation was found between related subscales of the two instruments.
Conclusion: The MPCOSQ has now been extensively tested in Iran and can be considered for using as an outcome measure in future outcome studies in this population.
Fatemeh Bazarganipour, Saeide Ziaei, Ali Montazeri, Fatemeh Foroozanfard, Anoshirvan Kazemnejad, Soghrat Faghihzadeh,
Volume 11, Issue 10 (12-2013)
Abstract
Background: Most previous research has focused on polycystic ovary syndrome (PCOS) characteristics and their association with psychological disorders, such as anxiety and depression. Objective: In the present study, our aim was to study whether PCOS characteristics are associated with several aspects of psychological well-being namely self-esteem and body satisfaction. Materials and Methods: This was a cross-sectional study of 300 women with PCOS that was carried out in Kashan, Iran. Main outcome measures were the Body Image Concern Inventory (BICI) and the Rosenberg’s Self-Esteem Scale and clinical information of PCOS. Major clinical PCOS features including obesity (BMI), excessive body hair (hirsutism score), acne, menstrual cycle disturbances and infertility. Results: The findings of regression analysis indicated that infertile women had lower levels of self-esteem (=-0.11, p=0.049) and poorer body satisfaction (=0.121, p=0.036) compared with PCOS women without infertility. Furthermore, hirsute women experienced poorer self-esteem than women without hirsutism (=-0.124, p=0.032). Women with menstrual irregularities had higher body dissatisfaction (=0.159, p=0.005). Moreover, women with higher body mass index scores had poorer body satisfaction (=0.151, p=0.009) but were not associated with self-esteem. Conclusion: The emotional well-being of the patients presenting with the syndrome needs to be recognized more fully, particularly in relation to the low self-esteem, poor body image, and struggles with weight, menstrual irregularities, hirsutism and infertility. The results of this study raise implications for clinical practice and suggest that a multidisciplinary approach to the management of women with PCOS.
Fatemeh Bazarganipour, Seyed Abdolvahab Taghavi, Ali Montazeri, Fazlollah Ahmadi, Reza Chaman, Ahmad Khosravi,
Volume 13, Issue 2 (2-2015)
Abstract
Background: Polycystic ovary syndrome (PCOS) has been shown to cause a reduction in health-related quality of life (HRQOL). However, the relative degree of impairment in each domain differed among samples, and it was not clear which aspect of disease-specific HRQOL (modified polycystic ovary syndrome health-related quality of life questionnaire) was most negatively affected.
Objective: To systematically review the effects of PCOS on specific domains of HRQOL.
Materials and Methods: Literature search using search engine of database (PubMed, PsychInfo, CINAHL, CENTRAL, and Scopus) between 1998 to December 2013 yields 6 relevant publications. Pairs of raters used structural tools to analyze these articles, through critical appraisal and data extraction. The scores of each domain of polycystic ovarian syndrome questionnaire (PCOSQ) or modified version (MPCOSQ) of 1140 women with PCOS were used in meta-analysis.
Results: The combine mean of emotional (4.40; 95% CI 3.77-5.04), infertility (4.13; 95% CI 3.81-4.45) and weight (3.88; 95% CI 2.33-5.42) dimensions were better, but menstruation (3.84; 95% CI 3.63-4.04) and hirsutism (3.81; 95% CI 3.26-4.35) domains were lower than the mean score of PCOSQ/MPCOSQ in related dimension.
Conclusion: The meta-analysis showed that the most affected domains in specific HRQOL were hirsutism and menstruation. Based on these findings, we recommend healthcare providers to be made aware that HRQOL impairment of PCOS is mainly caused by their hirsutism and menstruation, which requires appropriate management.
Seyed Abdolvahab Taghavi, Fatemeh Bazarganipour, Ali Montazeri, Anoshirvan Kazemnejad, Reza Chaman, Ahmad Khosravi,
Volume 13, Issue 8 (9-2015)
Abstract
Background: Increasing attention to the concept of polycystic ovary syndrome (PCOS) health-related quality of life has led to the development of tool that aims to measure this concept.
Objective: The purpose of this study was to conduct a systematic review of psychometric properties of the PCOS health-related quality of life questionnaire.
Materials and Methods: A search of database (Pubmed, PsychInfo, CINAHL, CENTRAL, Scopus and SID) from January1998 to December 2013 yielded 6152 references of which 27 papers remained after review of the titles and abstracts. The reviewers used structural tools to analyze the articles, critically appraise papers, and extract the data. Finally, eight papers met the full inclusion criteria.
Results: Studies suggested that the PCOS health-related quality of life questionnaire (PCOSQ)/or its modified version (MPCOSQ) have partial known groups validity. The convergent/divergent validity of the questionnaire also was found to be relatively acceptable. The PCOSQ/MPCOSQ reached acceptable benchmarks for its reliability coefficients. Regarding structural validity, some studies suggested that the PCOSQ/MPCOSQ have an extra dimension (related to menstruation) in addition to its existing dimensions for original or modified versions.
Conclusion: The PCOSQ/MPCOSQ showed acceptable content and construct validity, reliability and internal consistency. However, some other properties, particularly those related to factor and longitudinal validity, absolute error of measurement, minimal clinically important difference and responsiveness still need to be evaluated.
Farzaneh Fesahat, Fatemeh Montazeri, Mohammad Hasan Sheikhha, Hojjatollah Saeedi, Razieh Dehghani Firouzabadi, Seyed Mehdi Kalantar,
Volume 15, Issue 5 (6-2017)
Abstract
Background: Selection of the best embryo for transfer is very important in assistedreproductive technology (ART). Using morphological assessment for this selectiondemonstrated that the correlation between embryo morphology and implantationpotential is relatively weak. On the other hand, aneuploidy is a key genetic factorthat can influence human reproductive success in ART.
Objective: The aim of this lab trial study was to evaluate the incidence ofaneuploidies in five chromosomes in the morphologically high-quality embryosfrom young patients undergoing ART for sex selection.
Materials and Methods: A total of 97 high quality embryos from 23 women at theage of 37or younger years that had previously undergone preimplantation geneticscreening for sex selection were included in this study. After washing, the slides ofblastomeres from embryos of patients were reanalyzed by fluorescence in-situhybridization for chromosomes 13, 18 and 21.
Results: There was a significant rate of aneuploidy determination in the embryosusing preimplantation genetic screening for both sex and three evaluated autosomalchromosomes compared to preimplantation genetic screening for only sexchromosomes (62.9% vs. 24.7%, p=0.000). The most frequent detectedchromosomal aneuploidy was trisomy or monosomy of chromosome 13.
Conclusion: There is considerable numbers of chromosomal abnormalities inembryos generated in vitro which cause in vitro fertilization failure and it seems thatmorphological characterization of embryos is not a suitable method for choosing theembryos without these abnormalities.
Zohreh Sadat, Fazlollah Ghofranipour, Seyed Ali Azin, Ali Montazeri, Azita Goshtasebi, Azam Bagheri, Elham Barati,
Volume 16, Issue 1 (January 2018)
Abstract
Background: Designing a valid and reliable questionnaire that allows a fair evaluation of sexual knowledge and attitudes and develop a proper sexual educational program is necessary.
Objective: The present study was designed to develop and psychometric evaluation of the sexual knowledge and attitudes scale for premarital couples.
Materials and Methods: An exploratory mixed method study was conducted in two phases; in the first, in order to develop a questionnaire an item pool was generated on sexual knowledge and attitudes through focus group discussions and individual interviews. In the second phase, the psychometric properties of the questionnaire were examined. For this purpose, face validity, content validity as well as construct validity were conducted. Reliability was assessed by the Cronbach’s alpha coefficient to assess internal consistency and test-retest reliability.
Results: In the first phase an item pool with 88 questions was generated (sexual knowledge 45 items and sexual attitudes 43 items). In the second phase, the number of final items reduced to 33 and 34 items of sexual knowledge and sexual attitudes respectively, through exploratory factor analysis (EFA). Five factors for sexual knowledge and six factors for sexual attitudes identified by EFA. The Cronbach’s alpha coefficient for two sections was 0.84 and 0.81 respectively. The test- retest correlations for sexual knowledge and sexual attitude was 0.74 and 0.82 respectively.
Conclusion: The findings suggest that the Sexual Knowledge and Attitudes Scale for Premarital Couples is a valid and reliable instrument. Further studies are needed to establish stronger psychometric properties for the questionnaire.
Seyed Hamidreza Mirabutalebi, Noorodin Karami, Fatemeh Montazeri, Farzaneh Fesahat, Mohammad Hasan Sheikhha, Elnaz Hajimaqsoodi, Mojgan Karimi Zarchi, Seyed Mehdi Kalantar,
Volume 16, Issue 8 (August 2018)
Abstract
Background: The study of microRNA expression can be effective in the diagnosing and treating different diseases. miR-135a is one of the most important micro-ribonucleic acids involved in endometriosis. Among the genes that become the target of the miR-135a and are subjected to changes in the endometrium of patients with endometriosis is HOXA10 gene which is expressed in the endometrium in response to steroid hormones.
Objective: The aim of this study was to evaluate the expression of miR-135a and its relationship with the level of HOXA10 gene expression in both endometrial ectopic and eutopic tissues in patients with endometriosis compared to the control samples.
Materials and Methods: In this prospective case-control study, both case-eutopic and case-ectopic tissue samples were obtained from 17 women with endometriosis and the eutopic endometrial tissue was sampled from 17 women with normal endometrium as the control group. The gene's expression of miR-135a and HOXA10 were investigated using quantitative reverse transcription PCR (q-RT PCR).
Results: A significant decrease in the expression of HOXA10 gene was detected in case-eutopic during the luteal phase compared to the control samples (p=0.001), while in the case-ectopic, the expression of this gene was increased (p=0.681) compared to the control samples. In addition, the expression miR-135a in the luteal phase showed a remarkable increase in the case-eutopic endometrial tissue (p=0.026) as well as a significant decrease in the case-ectopic endometrial tissue compared to the control samples (p=0.008).
Conclusion: Considering the inverse relations between the over-expression of miR-135a and the reduction of HOXA10, it seems that miR-135a may be applied as an endometrial diagnostic and therapeutic biomarker.
Noorodin Karami, Seyed Hamidreza Mirabutalebi, Fatemeh Montazeri, Seyed Mehdi Kalantar, Mohammad Hasan Sheikhha, Maryam Eftekhar,
Volume 16, Issue 10 (October 2018)
Abstract
Background: Recurrent miscarriage, as the occurrence of two or more of pregnancy loss before the 20th wk, can occur for multiple causes. One of the causes of miscarriage may be a defect in the process of angiogenesis because the delivery of nutrients to the fetus is decreased and it may lead to miscarriage. Also, micro ribonucleic acids play an important role in the development of diseases. The microRNAs 16 and 21 are the most well-known angiogenesis-related miRNAs, which their gene targets are vascular endothelial growth factor-A and phosphatase and tensin homolog, respectively. Objective: To evaluate the changes in expression of microRNAs 16 and 21 and their association with the gene targets in women with unexplained RM. Materials and Methods: In this case-control study, blood samples were taken from 25 women with unexplained RM and 25 controls. After extraction of RNA, the relative expression of microRNAs and their gene targets was measured using real-time quantitative reverse transcription-PCR method.
Results: Our findings showed that miR-21 expression was significantly decreased in both plasma and peripheral mononuclear cells (p=0.04 and p=0.02, respectively) and could be associated with the PTEN expression (p=0.03), however, there is no significant correlation between miR-16 and VEGF-A. Conclusion: One of the most remarkable results of this study is that miR-21 showed significant changes in both plasma and peripheral mononuclear cells, which can be related to the etiology and progression of RM.
Seyed Mehdi Hoseini, Fateme Montazeri, Maryam Moghaddam-Matin, Ahmad Reza Bahrami, Hassan Heidarian Meimandi, Saeed Ghasemi-Esmailabad, Seyed Mehdi Kalantar,
Volume 18, Issue 10 (October 2020)
Abstract
Background: The genomic stability of stem cells to be used in cell therapy and other clinical applications is absolutely critical. In this regard, the relationship between in vitro expansion and the chromosomal instability (CIN), especially in human amniotic fluid cells (hAFCs) has not yet been completely elucidated.
Objective: To investigate the CIN of hAFCs in primary and long-term cultures and two different culture mediums.
Materials and Methods: After completing prenatal genetic diagnoses (PND) using karyotype technique and chromosomal analysis, a total of 15 samples of hAFCs from 650 samples were randomly selected and cultured in two different mediums as AmnioMAX II and DMEM. Then, proliferative cells were fixed on the slide to be used in standard chromosome G-banding analysis. Also, the senescent cells were screened for aneuploidy considering 8 chromosomes by FISH technique using two probe sets including PID I (X-13-18-21) & PID II (Y-15-16-22).
Results: Karyotype and interphase fluorescence in situ hybridization (iFISH) results from 650 patients who were referred for prenatal genetic diagnosis showed that only 6 out of them had culture- derived CIN as polyploidy, including mosaic diploid-triploid and diploid-tetraploid. Moreover, the investigation of aneuploidies in senesced hAFCs demonstrated the rate of total chromosomal abnormalities as 4.3% and 9.9% in AmnioMAX- and DMEM-cultured hAFCs, respectively.
Conclusion: hAFCs showed a low rate of CIN in two AmnioMAX II and DMEM mediums and also in the proliferative and senescent phases. Therefore, they could be considered as an attractive stem cell source with therapeutic potential in regenerative medicine.
Mansouri N, Hajari Ma, Sadeghi Abandansari H, Niknejadi N, Heidari Khoei H, Baharvand H, Montazeri L,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Severe trauma of uterus may cause damage in the basal layer of endometrium leading to intrauterine adhesion (IUA) or Asherman’s syndrome (AS), and eventually infertility. Nowadays, conventional approaches including surgical adhesiolysis and following hormone therapy are used to treat AS in clinics. However, the high recurrence after these procedures reveals the importance and superiority of IUA prevention instead of treatment. As a preventive agent, auto-crosslinked hyaluronic acid (HA) hydrogels can provide an anti-adhesive barrier to decrease the incidence of IUA. In addition, MSC-derived extracellular vesicles (EVs) have been recently introduced as an effective and novel therapeutic agent to reduce inflammation and fibrosis. This study has been designed to investigate whether combining the auto-crosslinked HA hydrogel with mesenchymal stem cell-extracellular vesicles (MSC-EVs) could improve the efficiency of HA in endometrial regeneration in the rat model of AS.
Objective: To evaluate whether the combination of HA hydrogel and MSC-EVs could facilitates functional regeneration of injured uterus in experimental rats.
Materials and Methods: Forty eight-week-old female Wistar rats weighting 200–250 g were randomly assigned into 5 groups (n = 8/each): I) Intact group: without any intervention, II) AS Model group: model was established by three surgical steps of mechanical injury (incision, curettage, and suture), III) Sham surgery group: Subjected to the abdominal surgery, incisions and suturing, but not the curettage procedure, IV) HA + MSC-EVs: A mixture of 400 μl HA hydrogel + 160 μg/kg/dose MSC-EVs (around 200 μl hydrogel containing 20 μg EV per each horn) was injected into the uterine horn immediately after making the AS model and V) HA: Receiving an intrauterine injection of only 400 μl HA hydrogel immediately after making the AS model. Two weeks after the transplantation, four rats from each group sacrificed and uterine samples were harvested to be evaluated histologically by H&E and Masson’s trichrome staining. The remaining four animals in each group were coupled with fertile males (female: male = 2:1) 1 week after modeling for 3 months. The number of deliveries and the cumulative number of pups were assessed at the end of this time to survey reproductive function.
Results: Histology examination revealed significantly thicker endometrium, increased gland numbers and fewer fibrotic areas in the HA + MSC-EVs and HA transplantation groups compared with the model group. The cumulative number of pups and number of deliveries also showed a significant increase in the HA group compare to the model group. But our results displayed no significant differences between the HA + MSC-EVs and HA groups in terms of morphometric parameters and mating test outcomes.
Conclusion: MSC-EVs cannot amplify preventive properties of HA on the rat model of AS.
Shafienia H, Montazeri F, Heydari L, Khalili Ma, Sheikhha Mh, Mazloomzadeh S, Biglari A,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. One of the problems in IVF cycles in PCOS women is predisposing to develop ovarian hyperstimulation syndrome. Therefore, in vitro maturation (IVM) of the oocytes has grown as an alternative treatment. Transcriptomic signatures of cumulus cells (CC) have the potential to serve as valuable non-invasive biomarkers for oocyte competence. Recent studies suggest miRNA involvement in regulating follicular growth, differentiation and development. miR-144 is one of the miRNAs that has been shown to involve in oocyte maturation.
Objective: In this study, the expression level of miR-144 and cyclooxygenase-2 (COX-2) as its candidate target gene was examined in women with PCOS, then its impact on IVM outcome of oocyte was evaluated.
Materials and Methods: A total of 30 cumulus-oocyte complexes with oocyte at GV stage were retrieved from 20 women with PCOS during IVF cycles and cultured in IVM medium for 24 hr at 37°C. After IVM, maturity of oocytes was assessed through morphological criteria and the samples were divided into two groups: matured and unmatured oocytes. The expression level of miR-144 and COX-2 in CCs of each group were detected by qRT-PCR and the relation between the expression level of them and IVM of oocytes was evaluated.
Results: In the 30 retrieved GV oocytes, 18 oocytes (60%) were matured after IVM and placed in matured group, whereas 12 oocytes (40%) could not mature and placed in U group. We found that the expression level of miR-144 was lower (P-value: 0.0008) and the COX-2 mRNA level was higher (P-value: 0.005) in CCs of matured group than in CCs of unmatured group. So, the selected miRNA was related to oocyte nuclear maturation in PCOS women.
Conclusion: We determined that the expression profile of miR-144 and COX-2 were different in CCs isolated from oocytes that could mature after IVM compared with those that could not in PCOS women. Since oocyte competence has an important role in formation of normal zygote and blastocyst, the expression level of this miRNA can be used for predicting oocyte quality before IVM process.
Zare E, Javid A, Hoseini Sm, Montazeri F,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Stem cells are undifferentiated cells that have the ability to transform and differentiate into different cell types. These cells have two important characteristics that differentiate them from others. They have the ability to reproduce unlimited and remain in the undifferentiated state. The most important mature types of these cells are mesenchymal cells, which become more susceptible to accumulation of cell damage with passing time and increase longevity. These damages can help to improving recovery, senescence and finally death of cells. Various factors cause the intrinsic and harmful process of senescence such as internal factors as genetics, the expression of some genes as P53, Nuclear factor NF-kappa-B and Forkhead Box M1 (FoxM1). free radicals and external factors such as environmental changes that affect the function of the cell. FoxM1 is a member of the Forkhead transcription family, which has been actively involved in regulating organism growth, differentiation and cell proliferation and is important for the expression of cell cycle-dependent genes in the G2 phase.
Objective: The main goal of this study was to evaluate the expression level of FoxM1 gene as a marker of senescence in mesenchymal stem cells isolated from human amniotic fluid at different passages.
Materials and Methods: Totally 37 amniotic fluid samples were obtained from pregnant mothers referred to the PND Department of Yazd Reproductive Sciences Institute. After culturing successive passages and examining the cells morphologically and characterizing them by flow cytometry, their aging status was evaluated in several passages by using beta-galactosidase (X-gal Cinna Gene) staining. After RNA extraction by Tripure kit and cDNA synthesis by Thermo Fisher kit, 10 samples in passages 4 and 7 were evaluated for FoxM1 gene expression change as a marker of aging and GAPDH gene as internal reference using (quantitative PCR) technique. The data were analyzed using GraphPad Prism and SPSS version16 software.
Results: Microscopic examination and staining of beta-galactosidase showed that mesenchymal stem cells isolated from amniotic fluid enter the aging stage in different passages. Comparing the results of FoxM1 gene expression in different passages (2, 4, and 7), showed a statistical meaningful increase of expression in old cells compared to young cells (F = 10.43; P < 0.001). Despite the increased expression of FoxM1 gene in the passage 4 compared to the young cells in passage 2, indicated that there was no significant difference between two groups (t = 1.134, p<0.05). Comparison of FoxM1 gene expression in aging cells of passage 7 compared with young cells of passage 2, showed that the increase was statistically significant (t = 3.758; p < 0.003).
Conclusion: FoxM1 gene expression in cellular aging has an effective role in preventing cellular aging, and control of aging-related traits includes reducing cell doubling time, regenerative power, and differentiation potential.
Hojati S, Miresmaeili Sm, Montazeri F, Amiri S, Hoseini Sm, Kalantar Sm, Fesahat F,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Thin long tail deletion of Y chromosome is the most common molecular genetic cause of infertility. It is considered to be severe in men which occurs in the three region of the azoospermic factor; AZFa, AZFb and AZFc. These region contain multiple genes involved in spermatogenesis.
Objective: The aim of this study was to investigate the Y chromosome deletion pattern among infertile men with globozoospermic referring to Yazd Infertility Treatment Center.
Materials and Methods: 19 infertile men referred to Yazd Reproductive Science Institute with globozoospermia (from 2014 to 2016) were studied considering microdeletions in Y chromosome. Using multiplex PCR and six different STS (Sequence-Tagged Site) markers microdeletions of Y chromosome in AZFa, AZFb and AZFc regions was analysed.
Results: In our samples, the deletion of AZF regions of the Y chromosomes was not abserved in any blood sample of globozoospermic man.
Conclusion: In 19 samples, no defect was observed in the AZF regions of the Y chromosomes was not the cause of globozoospermia.
Hossini Sm, Kalantar Sm, Aflatoonian B, Bahrami A, Montazeri F, Moghaddam Matin M,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: The amniotic fluid contains a heterogeneous population of different cells that are produced prior to the gastrulation process. Therefore, it is expected that mesenchymal stem cells derived from the amniotic fluid will have high plasticity between mature and pluripotent stem cells. Due to unique features of these cells such as high cloning potential, high self-renewal capacity along with chromosomal stability and low immunogenicity as well as anti-inflammatory and immune-modulating properties, it has attracted more and more attention from researchers.
Objective: The aim of this study was to investigate the immunosuppressive genes in mesenchymal stem cells isolated from amniotic fluid of women with a history of recurrent pregnancy loss (RPL) and the effect of gamma interferon as an immunological stimulus on the expression of these genes.
Materials and Methods: The study group included pregnant women with a history of unexplained RPL. The control group consisted of pregnant women with at least one healthy child, no history of miscarriage, and normal hormonal and immunologic profiles. In this study, mesenchymal stem cells (MSCs) isolated from amniotic fluid from RPL and non-RPL women. On the other hand, each cell line was examined under 5 different treatment groups, control and 4 groups with 20 and 100 IU IFN-γ per ml of medium over two periods of 24 h and 72 h. Finally, the relative mRNA expression level of immune-suppressive/modulator gene including two indole amine-2 and 3-dioxygenase 1 and 2 in AF-MSCs in both groups were evaluated and compared using Q-PCR.
Results: The average expression of candidate gene IDO1 and IDO2 showed a significant increase in the RPL group rather than non-RPL, specially under treatment with 100 IU IFN-γ and after 24 h. Interestingly, expression of both genes IDO1 and IDO2 decrease after 72 h in RPL and non-RPL groups (p = 0.05).
Conclusion: Immunosuppression by MSCs, which is currently recognized as a powerful tool in preventing acute rejection, graft therapy, and regenerative medicine, is not an inherent potential but is induced by environmental factors. Various studies have identified that some potential causes of unexplained RPL are due to immunological factors. The results of this study, especially for indole amine-2 and 3-dioxygenase genes, do not rule out such a possibility. Despite the unknown role of AF-MSCs in the abortion mechanism, the results of this study suggest that there is a significant difference between the mRNA level of understudied genes between AF-MSCs in the RPL and non-RPL group. Due to the absence of such a similar study, it cannot be fully interpreted, however, these cells appear to represent genetic compartments of couples with a history of RPL that may defective in immunological factors. However, planning for further investigation of these uncertain immunological mechanisms appears to be valuable in the future.
Jahanara M, Montazeri F, Sharifiyazdi H, Kalantar Sm, Hoseini Sm, Moshrefi M,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Chromosomal abnormalities are one of the most important causes of failure in in vitro fertilization. Preimplantation genetic testing can be a way to prevent the transfer of aneuploid embryos. It entails the use of invasive techniques to obtain embryonic DNA, with major technical limitations and ethical issues today. Therefore, the use of new non-invasive methods is a suitable solution to this problem. One of the non-invasive methods is to use the embryo spent culture medium. The origin of cell free DNA in embryo spent culture medium is trophectoderm cells and the internal cell mass.
Objective: Cell-free genomic DNA in the embryonic culture medium can be a non-invasive method for genetic assessment.
Materials and Methods: This study reviewed 25 spent embryo culture mediums. The spent culture medium used between day 3 and day 5 of embryonic development. Patients were undergoing intracytoplasmic sperm injection, and each embryo was in one drop of culture medium. We had two control samples: the culture medium contaminated with purified DNA from human blood and the culture medium without embryonic development. All samples were evaluated with nanodrop for dsDNA and ssDNA concentration. Among the collected medium, ten samples (group 1) concentrated by heating, then evaluating SRY and FMR1 genes with real-time polymerase chain reaction (RT-PCR) (group 1). Six samples were three days, and four samples were five days. The rest of the samples were classified into three groups. The cell-free DNA from the medium was purified with the blood DNA extraction kit. in group 2 with Genet bio kit, group 3 with YTzol pure DNA kit (yekta Tajhiz), and group 4 with High Pure Viral Nucleic Acid extraction kit (Roche). They evaluated by RT-PCR. Nine samples were three days, and six samples were five days.
Results: Although cell-free DNA was confirmed in the samples using nanodrop (with a range of 160 to 225 ng per microliter), the cycle of threshold did not observe in the RT-PCR product of group 1. The Purified samples were amplified in group 2,3 and 4 for SRY and FMR1 genes with RT-PCR and observed only acceptable cycle of threshold in the fourth group.
Conclusion: The high protein and solutes in the culture medium and the low amount and quality of DNA are restrictive. For better results, it is necessary to purify the genomic DNA and amplify it with precise kits. Our research is underway to improve DNA collection, amplification, and testing to isolate genomic DNA.
Aksoun M, Montazeri F, Hoseini Sm, Nikounahad N,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: One of the applications of human amniotic fluid mesenchymal stem cell is repairing nerve tissue. New studies have been conducted on the application of these cells in neural differentiation under different conditions including different concentrations of growth factors, different concentrations of amniotic fluid and various scaffolds. The results have been satisfactory and promising. Various factors have been optimized to improve the results of differentiation into neural tissues in these studies.
Objective: The purpose of this study was investigating the potential of human amniotic fluid mesenchymal stem cell in regenerative medicine special in neural tissue regenerating.
Materials and Methods: Evaluation of various studies performed in vitro and in vivo so far suggests the high differentiation potential of amniotic fluid mesenchymal stem cells. Considering the fact that the neural differentiation of these cells has received a lot of attention recently, the present study provides a complete overview of published research on the use of these cells in neural differentiation and neural tissue regeneration.
Results: Finding of review show amniotic fluid mesenchymal stem cell can alter morphological characteristic and become neural-like cells, which stimulate the expression of neuronal markers. Amniotic fluid stem cells showed a more primitive phenotype than the potential for differentiation of other stem cell sources, which could potentially be suitable for cell-based therapy in reconstructive medicine for neurodegenerative diseases.
Conclusion: According to the high potential of these cells in differentiation nerve tissue, as well limited studies on Human amniotic fluid mesenchymal stem cell for differentiation into neural tissue have been performed by the same research team in this obtained promising result.
Yazdi A, Montazeri F, Sheikhha Mh, Nikounahad N,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: In vitro fertilization (IVF) is a common approach for infertile couples with different etiologies. In a standard IVF cycle, exogenous follicle stimulating factor (FSH) is administered for ovarian stimulation. Numerous studies have been performed to determine individual variability in the ovarian response to gonadotropins in routine stimulation protocols. Different factors including hormonal FSH anti mullerian hormone, functional like antral follicle count (AFC), or genetic markers have been studied for optimizing individualized dose of exogenous gonadotropin in available protocols. Mutations in the genes coding for FSH receptor, luteinizing hormone receptor, estrogen receptor (ESR) and anti mullerian hormone receptor may influence ovarian response.
Objective: Considering the vital role of estrogen in ovulation, we aimed to investigate the SNP in position 1082 G/A (rs1256049) of the beta-estrogen receptor gene (ESR2) gene that may be correlated with an altered response to FSH in IVF cycles.
Materials and Methods: In this study, 200 women with poor ovarian resesponse and 40 women with good response were studied in terms of polymorphism RsaI 1082 G>A of ESR2 gene. Different genotypes of this polymorphism (GG, AG, AA) were determined using RFLP-PCR technique and using RsaI restriction enzyme.
Results: Statistical analysis was performed using SPSS software and appropriate statistical tests. Finding showed that there was no significant difference between the genotypic distribution of the under-studied polymorphism and the allele frequency with in and between groups.
Conclusion: Our finding suggests the polymorphism 1082 G > A of ESR2 gene is not related to poor ovarian response. However, the mechanism involved in this relationship is still unknown and considering the polymorphic nature of the ESR2gene (around 1800 SNP), more studies are needed to ascertain this relationship. In fact, more understanding of these genomic variants would help to managing controlled ovarian stimulation protocol, individually. Generally, the study of SNPs of the reproductive hormone receptor genes is a remarkable field of research that could provide us with new evidence about the way each woman responds to exogenous gonadotropin administration during ovulation induction.
Dehestani M, Montazeri F, Nikounahad N,
Volume 19, Issue 5 (Suppl- 2021)
Abstract
Background: Physiological function of ovarian regulates by reproductive hormones including estrogen. Estrogen is a steroidal hormone and its actions in ovary mostly occur through its binding to intracellular receptor α and β. ESR1 (α) gene include many polymorphic sites (SNPs) located along various regions of it that control expression and function of this receptor.
Objective: This paper aimed to investigate the association of XbaI A/G (rs9340799) with poor ovarian response in Iranian women undergoing IVF treatment referred to Yazd Reproductive Sciencse Institute.
Materials and Methods: To do so, a group of 40 women with normal response ovarian and a group of 209 women with poor response ovarian in IVF cycles were included. Genomic DNA extraction was performed with Blood DNA Extraction Kit (Favorgen Co.). Using PCR-FRLP technique and XbaI restriction enzyme SNP in -29 G/A site of ESR1 gene were genotyped.
Results: Our finding show polymorphisms in ESR1 (rs9340799) was significantly different between women with normal and poor response ovary considering AG+GG and GG+AA (p = 0.005) genotype but was not statisticaly signification regarding to AA+AG (p ≤ 0.05).
Conclusion: The study of SNPs of the ESR1 gene is an interesting field of research that could provide us with new facts considering the way each woman responds to standard stimulation protocol in IVF cycle.
