Showing 4 results for Hyaluronic Acid
Mitra Bakhtiari, Aligholi Sobhani, Mohammad Akbari, Parichehr Pasbakhsh, Mehdi Abbasi, Azim Hedayatpoor, Fardin Amidi , Feridoon Sargolzaei,
Volume 5, Issue 3 (7-2007)
Abstract
Background: Various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes.
Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing.
Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 µg/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate.
Results: HA supplementation (750 µg/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 µg/ml HA was also positive on the sperms. But 1,250 µg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.
Conclusion: The dose of 750 µg/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.
Seiede Zahra Ghanadzadeh Tafti, Atiye Javaheri, Razie Dehghani Firoozabadi, Samane Kabirpour Ashkezar, Hosain Falahzade Abarkoe,
Volume 19, Issue 4 (4-2021)
Abstract
Background: Adhesion due to intrauterine surgery such as septal resection associated with damaged endometrium can increase the risk of Asherman's syndrome. The main goal of treatment in this syndrome is to repair the damaged endometrium for creating a physiological pregnancy.
Objective: To investigate the effect of intrauterine injection of hyaluronic acid on the prevention of Asherman's syndrome in women undergoing uterine septum resection.
Materials and Methods: In this double-blind randomized clinical trial, 65 women undergoing the uterine septum resection were divided into two groups; the case group (n = 34) and the control group (n = 31). Immediately after the septal resection with a resectoscope, 1cc of hyaluronic acid gel in the case group and 1cc normal saline solution as a placebo in the control group was injected into the uterine cavity. After two months, existence of intrauterine adhesions in the both groups was examined by the hysteroscope and assessment of menstrual patterns, according to the American Society for Reproductive Medicine criteria.
Results: Our results showed that after intervention, the incidence of Asherman's syndrome in the control group was higher than the case group (p = 0.012). In the case group, only four women had poor adhesion (Asherman's syndrome) at the end of the study, while the rest of them were free of any adhesions in the uterine cavity. In the control group, however, only 19 were free of intrauterine adhesions and 12 had mild symptoms.
Conclusion: The results of the study exhibited the hyaluronic acid capacity to reduce the risk of Asherman's syndrome in women with endometrial damage following a septal resection surgery.
Mansouri N, Hajari Ma, Sadeghi Abandansari H, Niknejadi N, Heidari Khoei H, Baharvand H, Montazeri L,
Volume 19, Issue 5 (5-2021)
Abstract
Background: Severe trauma of uterus may cause damage in the basal layer of endometrium leading to intrauterine adhesion (IUA) or Asherman’s syndrome (AS), and eventually infertility. Nowadays, conventional approaches including surgical adhesiolysis and following hormone therapy are used to treat AS in clinics. However, the high recurrence after these procedures reveals the importance and superiority of IUA prevention instead of treatment. As a preventive agent, auto-crosslinked hyaluronic acid (HA) hydrogels can provide an anti-adhesive barrier to decrease the incidence of IUA. In addition, MSC-derived extracellular vesicles (EVs) have been recently introduced as an effective and novel therapeutic agent to reduce inflammation and fibrosis. This study has been designed to investigate whether combining the auto-crosslinked HA hydrogel with mesenchymal stem cell-extracellular vesicles (MSC-EVs) could improve the efficiency of HA in endometrial regeneration in the rat model of AS.
Objective: To evaluate whether the combination of HA hydrogel and MSC-EVs could facilitates functional regeneration of injured uterus in experimental rats.
Materials and Methods: Forty eight-week-old female Wistar rats weighting 200–250 g were randomly assigned into 5 groups (n = 8/each): I) Intact group: without any intervention, II) AS Model group: model was established by three surgical steps of mechanical injury (incision, curettage, and suture), III) Sham surgery group: Subjected to the abdominal surgery, incisions and suturing, but not the curettage procedure, IV) HA + MSC-EVs: A mixture of 400 μl HA hydrogel + 160 μg/kg/dose MSC-EVs (around 200 μl hydrogel containing 20 μg EV per each horn) was injected into the uterine horn immediately after making the AS model and V) HA: Receiving an intrauterine injection of only 400 μl HA hydrogel immediately after making the AS model. Two weeks after the transplantation, four rats from each group sacrificed and uterine samples were harvested to be evaluated histologically by H&E and Masson’s trichrome staining. The remaining four animals in each group were coupled with fertile males (female: male = 2:1) 1 week after modeling for 3 months. The number of deliveries and the cumulative number of pups were assessed at the end of this time to survey reproductive function.
Results: Histology examination revealed significantly thicker endometrium, increased gland numbers and fewer fibrotic areas in the HA + MSC-EVs and HA transplantation groups compared with the model group. The cumulative number of pups and number of deliveries also showed a significant increase in the HA group compare to the model group. But our results displayed no significant differences between the HA + MSC-EVs and HA groups in terms of morphometric parameters and mating test outcomes.
Conclusion: MSC-EVs cannot amplify preventive properties of HA on the rat model of AS.
Serajoddin Vahidi, Nima Narimani, Laleh Dehghan Marvast, Esmat Mangoli, Ali Nabi, Mohammad Sadeghi,
Volume 20, Issue 5 (5-2022)
Abstract
Background: The sperm DNA fragmentation index (DFI) is one of the men's reproductive health criteria that affects assisted reproductive technique outcomes. Efforts in obtaining high-quality mature sperms seem to be necessary. Advanced sperm selection techniques (including physiological intracytoplasmic sperm injection [PICSI], zeta potential, microfluidic, etc.) have gained popularity in this regard.
Objective: The study aimed to compare the efficacy of zeta potential and PICSI sperm selection in obtaining sperms with better DNA integrity.
Materials and Methods: In this cross-sectional study, 48 couples were enrolled where the male partner had increased sperm DFI in his ejaculated sample and the female was in normal reproductive health. For each male partner, the semen sample was processed with zeta potential and PICSI techniques, then the sperm DFI of neat semen was compared to zeta and PICSI samples by the sperm chromatin dispersion test.
Results: Data showed that both the zeta potential and PICSI technique decreased sperm DFI in comparison with the neat semen sample (p < 0.001 for both). In addition, there was a statistically significant difference in sperm DFI between the PICSI and zeta potential samples (p < 0.01).
Conclusion: The current study showed that both zeta potential and PICSI could result in sperm with a lower DFI. However, PICSI seems to be superior to zeta potential in this regard.
