Showing 16 results for Sperm Parameters
Mitra Bakhtiari, Aligholi Sobhani, Mohammad Akbari, Parichehr Pasbakhsh, Mehdi Abbasi, Azim Hedayatpoor, Fardin Amidi , Feridoon Sargolzaei,
Volume 5, Issue 3 (7-2007)
Abstract
Background: Various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes.
Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing.
Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 µg/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate.
Results: HA supplementation (750 µg/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 µg/ml HA was also positive on the sperms. But 1,250 µg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.
Conclusion: The dose of 750 µg/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.
Nasrin Sheikh, Iraj Amiri, Marzieh Farimani, Rezvan Najafi, Jafar Hadeie,
Volume 6, Issue 2 (7-2008)
Abstract
Background: It is established that sperm DNA integrity is essential in fertilization and normal embryo and fetal development. Routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm DNA. Therefore, the evaluation of sperm DNA integrity, in addition to routine sperm parameters, could add further information on the quality of spermatozoa and reproductive potential of males.
Objective: The objective of this study was to determine the levels of sperm DNA damage in fertile and infertile males and its correlation with semen parameters.
Materials and Methods: Semen samples were collected from 45 infertile men selected from couples attending the infertility clinic with a history of infertility of ≥1 years and 75 healthy volunteers of proven fertility (initiated a successful pregnancy) served as the control group. After routine sperm analysis, DNA damage was determined using single cell gel electrophoresis (comet) assay method.
Results: The mean of DNA damage (comet value) in the sperms of infertile males was significantly higher than that of fertile males (12.9±7.59 vs. 48.77±24.42, p<0.001). A significant negative correlation was observed between DNA damage and sperm motility in fertile group (p<0.02, R=-0.263). In infertile males, significant negative correlations were observed between DNA damage with sperm motility (p<0.002, R=-0.45) and morphology (p<0.03, R=-0.317). There was no significant correlation between sperm concentration and sperm DNA damage in both groups.
Conclusion: These results indicate that sperm DNA damages in infertile males is significantly higher than fertile males and sperms with abnormal morphology and low levels of motility has more abnormal DNA damages than motile and normal sperms.
Seyed Gholam Ali Jorsaraei, Hiroaki Shibahara, Ayustawati , Yuki Hirano, Yasuko Shiraishi, Ali Khalatbari, Yousofreza Yousofnia Pasha, Mitsuaki Suzuki,
Volume 6, Issue 4 (7-2008)
Abstract
Backgrand: Cotinine (COT) is a major degradation product of nicotine (NIC). The participation of leptin in female reproduction is well-established, but any role in male reproductive function is at the best tenuous.
Objective: The aim of this study was to evaluate the in-vitro effects of nicotine, cotinine and leptin on sperm parameters in normal semen of non-smokers fertile men.
Materials and Methods: Ten healthy nonsmokers aged 25-40 years old were devided into 7 groups, Thier semens were divided into 7 aliquots. (A) was layered with basal solution 70 ng/ml NIC, (B) 35 µg/ml NIC, (C) 300 ng/ml COT, (D) 200 µg/ml COT, (E) 30 ng/ml leptin, (F) 300 ng/ml leptin respectively and (G) was layered with mHTF. After migration, the samples were examined at time 0, +1, +2, +4, +8, and + 24 h of incubation.
Results: These findings were obtained: sperm count: 75.66±66.25x10 6 /ml, forward motility: 75.55±14.80%, progress: 33.66±13.01, VSL: 51.58±6.99 µm/s, VCL: 103.33±14.52 µm/s, ALH: 4.33±0.77 µm, BCF: 25.60±2.97 HZ, STR: 79.33±8.04 %, LIN: 52.55±10.52 %, ELO: 74.22 ± 12.76 % and ARE: 3.04 ± 1.50 u/sq. The parameters were similar before 8 hr and were being decreased after that.
Conclusion: According to the results. nicotin and cotinin have negative effects on the sperm parameters but despite the positive effect of leptin, there is no correlation between leptin concentration in semen and its physical characteristics.
Hamid Reza Momeni, Malek Soleimani Mehranjani, Mohammad Hosien Abnosi, Monireh Mahmoodi,
Volume 7, Issue 3 (7-2009)
Abstract
Background: para-nonylphenol (p-NP) is able to induce malformations in male reproductive system.
Objective: The aim of this study was to investigate the preventing role of vitamin E (Vit.E) on sperm parameters and reproductive hormones in developing rats.
Materials and Methods: Pregnant rats were divided into 4 groups: control p-NP Vit.E and p-NP+Vit.E. Treatments were performed on day 7 of gestation and continued during weaning. The male pups were then divided into the same groups as the mothers and were treated till 90 days of age. Finally body and left testis weight were recorded and left epididymis was cut in Ham’s F10. Released sperm were used to analyze number motility and viability of the sperm. Blood serum was used to assess follicle stimulating hormone (FSH) luteinizing hormone (LH) estrogen and testosterone.
Results: In p-NP-treated rats a significant decrease was found in body and testis weight sperm number and sperm motility compared to control and p-NP+Vit.E groups. A significant increase was also found in sperm viability in Vit.E group (83.3±7.6) compared to both p-NP (59.5±7.5) and control (66.3±9.7) groups. Rats treated with p-NP showed a significant decrease in FSH level and a significant increase in estrogen level. However testosterone and LH level remained constant. In p-NP+Vit.E group the change of estrogen level but not FSH was significantly reversed compared to p-NP group. Conclusion: Vit.E not only is able to compensate the toxic effects of p-NP on testis weight sperm number sperm motility and estrogen level but also increases sperm viability in developing rat.
Hamid Reza Momeni, Najmeh Eskandari,
Volume 10, Issue 3 (7-2012)
Abstract
Background: Arsenic as an environmental toxicant is able to exert malformations in male reproductive system by inducing oxidative stress. Vitamin E (Vit.E) is known as antioxidant vitamin.
Objective: The aim of this study was to investigate the harmful effects of sodium arsenite on sperm parameters and the antioxidant effects of Vit.E on sperm anomalies in sodium arsenite treated rats.
Materials and Methods: Adult male rats were divided into 4 groups: control, sodium arsenite (8 mg/kg/day), Vit.E (100 mg/kg/day) and sodium arsenite+Vit.E. Oral treatments were performed till 8 weeks. Body and left testis weight were recorded and then left caudal epididymis was cut in Ham's F10. Released spermatozoa were used to analyze number, motility, viability and abnormalities of the sperm. Sperm chromatin quality was assessed by nuclear staining using acridine orange and aniline blue.
Results: Body and testis weight showed no significant change in 4 groups (p>0.05). A significant decrease in the number, motility, viability and normal sperm morphology was found in sodium arsenite-treated rats compared to the control (p<0.001). Sodium arsenite had no effect on sperm DNA integrity and histon-protamine replacement (p>0.05). In sodium arsenite+Vit.E group, Vit.E could significantly compensate the harmful effects of sodium arsenite on sperm number, motility, viability and morphology compared to sodium arsenite group. In addition, sperm viability and motility was significantly increased in rats treated with Vit.E alone compared to the control and sodium arsenite+Vit.E group.
Conclusion: Vitamin E could compensate the adverse effects of sodium arsenite on sperm parameters in adult rats.
Mohammad Ebrahim Baki, Sayyed Mohsen Miresmaili, Majid Pourentezari, Esmail Amraii, Vahid Yousefi, Hamid Reza Spenani, Ali Reza Talebi, Morteza Anvari, Mohammad Fazilati, Ali Asghar Fallah, Esmat Mangoli,
Volume 12, Issue 2 (2-2014)
Abstract
Background: Nano-particles are extensively employed in most industries. Several studies have been started to explore the probable detrimental effects of nano-particles on human reproduction. However, there is insufficient and controversially evident of effects of silver nano-particles on sperm parameters and other reproductive indices.
Objective: Investigation of the effects of silver nano-particles on sperm parameters, sex hormones and Leydig cells in rat as an experimental model.
Materials and Methods: In this experimental study, 75 male prepubertal Wistar rats were categorized in five groups including control group and 4 experimental groups (n=15 in each group). The rats in the experimental groups were fed silver nano-particles (60 nm in dimension) with concentrations of 25, 50, 100, and 200 mg/kg/day. After 45 days (about one duration of spermatogenesis in rat), samples of blood were taken from the rats for testosterone, leuteinizing hormone (LH), and follicle stimulating hormone (FSH) assessments. Afterwards, the epididymis and the testis of each rat were dissected for analyzing sperm parameters and Leydig cells.
Results: The results demonstrated a statistically significant reduction in number of Leydig cells in experimental groups compared to control one. In addition, the data showed a reduction in testosterone and a rise in LH level which was more obvious in high doses (p<0.05); however, FSH level showed a reduction but it was not statistically significant. A significant decrease was also found in sperm motility and normal sperm morphology in the experimental groups compared to the control one.
Conclusion: Our results demonstrated that silver nano-particles, in addition to interruption in functions of sex hormones, can diminish the number of Leydig cells and sperm parameter indices. It should be noted that the effects of nano-particles on reproductive indices are dose-dependent.
Cyrus Jalili, Mohammad Reza Salahshoor, Ali Naseri,
Volume 12, Issue 6 (8-2014)
Abstract
Background: Nicotine consumption can decrease fertility drive in males by inducing oxidative stress and DNA damage. Urtica dioica L (U.dioica) is a multipurpose herb in traditional medicine for which some anti-oxidative and anti-inflammatory properties have been identified.
Objective: The main goal is to investigate whether the U.dioica could inhibit nicotine adverse effects on sperm cells viability, count, motility, and testis histology and testosterone hormone.
Materials and Methods: In this study, hydro-alcoholic extract of U.dioica was prepared and various doses of U.dioica (0, 10, 20, and 50 mg/kg) and U.dioica plus nicotine (0, 10, 20, and 50 mg/kg) were administered intraperitoneally to 56 male mice for 28 consequent days. These mice were randomly assigned to 8 groups (n=7) and sperm parameters (sperm cells viability, count, motility, and morphology), testis and prostate weight, testis histology and testosterone hormone were analyzed and compared.
Results: The results indicated that nicotine administration (0.5 mg/kg) significantly decreased testosterone level, count and motility of sperm cells, and testis weight compared to control group (p=0.00). However, increasing the dose of U.dioica significantly boosted motility, count, normal morphology of sperm cells, seminiferous tubules diameter, and testosterone in all groups compared to control (p=0.00) and testis weight in 20 and 50 mg/kg doses in comparison with control group (p=0.00).
Conclusion: It seems that U.dioica hydro-alcoholic extract administration could increase the quality of spermatozoa and inhibits nicotine-induced adverse effects on sperm parameters.
Pegah Kargar- Dastjerdy, Marziyeh Tavalaee, Mansoor Salehi, Mojtaba Falahati, Tayebeh Izadi, Mohammad Hossein Nasr Esfahani,
Volume 14, Issue 1 (1-2016)
Abstract
Background: KLC3 protein as a member of the kinesin light-chain protein family plays an important role in spermatogenesis, during formation of mitochondrial sheath in the mid piece of the sperm tail.
Objective: This study for the first time aims to compare the expression of the KLC3 gene between fertile and infertile individuals.
Materials and Methods: Semen samples were collected from 19 fertile individuals who were selected from embryo-donor volunteers and 57 infertile individuals who had abnormal sperm parameters according to world health organization criteria. Sperm parameters using computer assisted sperm analysis and the quantitative KLC3-gene expression using the real-time PCR method were measured.
Results: Our results revealed a significant correlations between sperm concentration with relative expression of KLC3 only in infertile groups (r=0.45, p=0.00). A significant correlation was not found between KLC3 expression and sperm motility; however, the relative expression of KLC3 was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals.
Conclusion: Low expression of KLC3 may result in improper function of midpiece, which has important function in sperm motility. The results of this study show that aberrant expression of KLC3 might be associated with phenomena like oligozoospermia and asthenozoospermia. This article is extracted from student’s thesis.
Maryam Zohour Soleimani, Farideh Jalali Mashayekhi, Morteza Mousavi Hasanzade, Maryam Baazm,
Volume 16, Issue 3 (3-2018)
Abstract
Background: CatSper gene, a member of cation channel sperm family, has an essential role in sperm motility and male fertility. Following varicocele, sperm parameters especially sperm movement decreases. For this reason, we hypothesized that CatSper gene expression might be reduced after varicocele induction in an animal model.
Objective: The aim of this study was to evaluate the expression of CatSper 1 and 2 genes, sperm parameters and testis histology following varicocele induction.
Materials and Methods: A total of 30 Wistar male rats were randomly divided into three following groups (n=10/ each): control, sham, and varicocele group. Experimental varicocele was induced by partial ligation of the left renal vein. The epididymal sperm parameters, CatSper1 and 2 genes expression, and testes histology were studied two months after varicocele induction.
Results: Our results revealed that motility (32.73±16.14%), morphology (48.80±17%) and viability (31.23±9.82%) of sperms significantly reduced following varicocele induction. In addition, we showed a significant decrease in the number of spermatogonia (43.63±5.31) and seminiferous tubules diameters (190.51±19.23 mm) in experimental varicocele rats. The level of CatSper1 and 2 genes expression evaluated using real-time polymerase chain reaction was significantly downregulated 2 months after varicocele induction.
Conclusion: Our data indicated that experimental varicocele has deleterious effects on sperm parameters, testis structure as well as the expression of CatSper 1 and 2 genes.
Haeri F, Shirani M, Nouri M, Dehghan Marvast L, Ghiasvand R,
Volume 19, Issue 5 (5-2021)
Abstract
Background: Some epidemiological studies have reported a relationship between infertility and lifestyle patterns including dietary habits.
Objective: Our objective was to identify the relation between sperm parameters and dietary fatty acid and mineral intake among Iranian infertile men.
Materials and Methods: This cross sectional was performed on 400 newly diagnosed infertile men in Yazd Reproductive Sciences Institute from July to December 2019. Men were recruited when their infertility was confirmed by the expert andrologist based on World Health Organization criteria. They delivered a semen sample and answered a 168 items semiquantitative food frequency questionnaire. All data were analyzed using SPSS V. 22 software. P-value less than 0.5 considered as significant.
Results: We found a positive association between poly-unsaturated fatty acid intake, total motility, and normal morphology (p = 0.03). Also, there was a significant negative association between the second quartile of sodium and calcium intake and sperm volume (ptrend: 0.04), compared with the first quartile.
Conclusion: We concluded that dietary of poly-unsaturated fatty acid intake, sodium and calcium intake are related to sperm morphology, volume and total motility in Iranian infertile men. However, more research is needed to confirm these relations and provide the evidence needed to exert these findings into clinical practice.
Koohestanidehaghi Y, Torkamanpari M, Shirmohamadi Z, Lorian K, Vatankhah M,
Volume 19, Issue 5 (5-2021)
Abstract
Background: Assuming the adverse effects of reactive oxygen species (ROS) on sperm function, this study was conducted to assess the effects of cysteine and glutamine as effective antioxidants on human sperm parameters under vitrification.
Objective: The present study aimed to investigate the protective effect of cysteine and glutamine on motility parameters, plasma membrane potential, mitochondrial membrane potential, DNA damage and human sperm intracellular ROS during vitrification.
Materials and Methods: Twenty normozoospermic samples were used. The samples were subjected to a vitrification process and cysteine (5 and 10 mM) and glutamine (10 and 15 mM). The sperm motility parameters, mitochondrial membrane potential (MMP), plasma membrane integrity (PMI), DNA damage and intracellular ROS damage were assessed for each sample.
Results: Statistical analyses showed that motility, mitochondrial membrane potential and DNA damage decreased in the vitrified groups with cysteine 5, 10 mM and glutamine 10, 15 mM separately. Also intracellular ROS increased significantly compared to the fresh group (p < 0.05). No significant differences were observed for PMI compared with the fresh group (p > 0.05). Supplementation of cysteine and glutamine in both concentrations separately decreased intracellular ROS and DNA damage of spermatozoa with significant increase in PMI, MMP and progressive motility compared to vitrified control group (p < 0.05).
Conclusion: The results showed no significant effect of a specific concentration in cysteine and glutamine on sperm parameters compared to other concentrations. Both amino acids have the potential to improve the harmful effects of freezing on sperm parameters.
Pourentezari M, Dehghan M, Ashoorzadeh S, Talebi Ar,
Volume 19, Issue 5 (5-2021)
Abstract
Background: So far, the effects of blunt trauma on sperm parameters and reproductive capacity have not been firmly established and diverse reports have been presented.
Objective: The aim of this study was to investigate the effect of unilateral blunt testis on sperm parameters in acute and chronic periods after injury in mice.
Materials and Methods: In this study, 40 adult male NMRI mice with a weight of 35-30 gram were selected randomly and divided into 3 groups: control, sham (Mice in this group were only surgically treated) and experimental (Mice were surgically treated in this group and the blunt was hit by their left testes). Sampling was performed in two acute (48 hours after surgery) and chronic (1 month and 2 months after surgery), after anesthesia the tail of epididymis was separated and placed in Ham's F10 solution. Then sperm samples were examined microscopically in terms of motility, number (with 40x lens), viability (eosin stain color and hypoosmotic swelling).
Results: In the case of rapid sperm motility, the control group with acute experimental, one and two month chronic, acute sham group with sham one month, acute and chronic experimental one and two months, and sham group two months with acute experimental groups and sham one and two months were significant. Regarding the sperm viability, the control group with one month acute and chronic sham, the acute sham group with one month sham groups, an acute and chronic one month experimental, and a one month chronic group with acute and chronic two month experimental groups were significant. Regarding sperm count, a one month chronic group with control groups, acute sham, one to two months sham and an acute experimental with one month chronic group was significant.
Conclusion: The testicular blunt has affected sperm parameters. In other words, the mean number of sperm (ml /ml) and the percentage of sperm survival and motility (progressive, non-progressive, and inactivity) were significant between the control and sham groups and the experimental group, which was not effective on fertility.
Ghasemi-Esmailabad S, Talebi Ah, Talebi Ar, Amiri S, Moshrefi M, Pourentezari M,
Volume 19, Issue 5 (5-2021)
Abstract
Background: Morphine is one of the major psychoactive chemicals in opium that can increase the production of free radicals and thus can negatively affect spermatogenesis.
Objective: The purpose of this survey was to demonstrate the effect of morphine consumption on sperm parameters, DNA integrity and apoptosis in men taking morphine.
Materials and Methods: In this case-control study, 30 man abusing morphine (cases) and 30 healthy men (controls) were compared for sperm parameters (count, motility and morphology) and sperm chromatin quality, with aniline blue, toluidine blue and Chromomycin A3 stainings. The participants were matched for age, weight, amount and duration of cigarette smoking.
Results: In men with morphine dependency, sperm progressive and total motility (p = 0.038 and p < 0.001 respectively) showed significant decreasing compared to control group. Regard to morphine abusing, although morphine can decrease the sperm chromatin condensation and increases the rate of sperm apoptosis, but these differences were not statistically significant.
Conclusion: According to our result morphine dependence can reduce male fertility by affecting sperm parameters and also it may affect sperm chromatin/DNA integrity.
Pouriayevali F, Tavalaee M, Nasr-Esfahani Mh,
Volume 19, Issue 5 (5-2021)
Abstract
Background: Diabetes mellitus could have multiple effects on various organs of the body. One of the organs that are sensitive to these effects is testis and spermatogenesis process.
Objective: Aim of this study was to compare the effects diabetes type 1 (DM1) and type 2 (DM2) on sperm parameters.
Materials and Methods: Forty male mice C57 (8 weeks; 22 gr) were divided into 4 groups (n = 10/each). Mice were fed with standard-chow diet except DM2 group that was fed with a 60%-kcal high-fat diet for 8 weeks. Furthermore, sham group received a single dose of sodium citrate buffer (0.005 mg/kg) as soluble of streptozotocin (STZ), DM1 group was induced by multiple low-dose injections of STZ (45 mg/kg/day for 5 consecutive days), and DM2 group after four weeks was given a single dose of STZ (110 mg/kg). After eight weeks, the mice were sacrificed and sperm was extorted from the cauda epididymis for tests on sperm parameters.
Results: This study showed that the effects of diabetes on sperm parameters were compared between groups. The mean percentage of sperm non progressive motility significantly was higher in DM2 group than control group (p = 0.05), however sperm total motility difference between groups wasn’t remarkable. Moreover, the mean percentage of sperm concentration was lower in DM1 group compared to other groups (p < 0.02).
Conclusion: Sperm parameters in type 1 and 2 diabetes mellitus male mice C57 could effect on reproductive system. This result showed that reduction of sperm concentration and progressive motility of sperm in DM1 and DM2 model mice were lower compared to control group.
Doostabadi M, Hassanzadeh-Taheri M, Asgharzadeh M,
Volume 19, Issue 5 (5-2021)
Abstract
Background: Excessive consumption of alcohol induces increasing in oxidative stress production and can lead to detrimental effects in the male reproductive system.
Objective: The aim of the present study was to evaluate possible protective effects of co-administration of vitamin E, as a well-known antioxidant, on detrimental changes of sperm quality in mice intaken ethanol.
Materials and Methods: In this experimental study, 54 BALB/c mice were categorized into 9 groups (n = 6 /each). Group 1: The Control group received a basal diet. Groups 2, 5: Gavaged with 10% and 20% (V/V) ethanol (99% v/v, Merk, Germany) daily, respectively. Groups 3, 4: Gavaged with 10% (V/V) ethanol and injected with Vitamin E (Osveh Co., Iran) 100, 200 mg/kg intraperitoneally, respectively. Groups 6, 7: Gavaged with 20% (V/V) ethanol and injected with Vitamin E 100, 200 mg/kg intraperitoneally, respectively. Groups 8, 9: Received Vitamin E 100, 200 mg/kg intraperitoneally, respectively. The control group received basal diet and experimental groups including (alcohol 10% & 20%, alcohol 10% vit.E 100 & 200 mg, alcohol 20%/ vit.E 100 & 200 mg and vit.E 100 & 200 mg). After 35 days, the epididymis was dissected for analyzing sperm parameters include sperm motility, morphology, and viability. Sperm chromatin was assessed with Aniline Blue and Toluidine Blue staining. TUNEL assay was performed to evaluate the extent of DNA damage in spermatozoa.
Results: The results demonstrated a statistically significant reduction in motility rate (p = 0.04), normal morphology rate (p < 0.0001 and p < 0.0001, respectively), viability rate (p < 0.0001 and p < 0.0001, respectively), increase abnormal DNA structure and packaging Toluidine Blue staining (p = 0.01) and DNA damage (TUNEL) (p = 0.04) in ethanol consumer groups compared to the control. In addition, the findings showed a significant increase in the above-mentioned parameters in ethanol and vitamin E consumer group compared to the counterpart ethanol consumer groups. However, the extent of protamine deficiency Aniline Blue was not different in any experimental group compared to the control. The ethanol group received 20% of the most damage among the groups. The group receiving vitamin E 100 mg/kg and the group receiving ethanol 10% with vitamin 200 mg/kg gained the highest benefit among the groups.
Conclusion: Result showed that sperm forward progressive motility, normal morphology rate and viability decreased significantly in ethanol (10% and 20%) treated groups when compared with the control group also the rates of spermatozoa with abnormal DNA structure and DNA fragmentation increased significantly in the ethanol intake group than the control group. While, co-treatment with vit. E could prevent some of these adverse effects. Our findings in the current study revealed that co-administration of vitamin E and ethanol can protect destructive changes in DNA structure and damage.
Key words: Ethanol, Sperm parameters, Vitamin E.
Mohamadreza Doostabadi, Mohammadmehdi Hassanzadeh-Taheri, Mahmoud Asgharzadeh, Masoomeh Mohammadzadeh,
Volume 19, Issue 6 (6-2021)
Abstract
Background: Excessive consumption of alcohol induces an increase in oxidative stress production and can lead to detrimental effects on the male reproductive system.
Objective: To evaluate the possible protective effects of co-administration of vitamin (vit) E on the detrimental changes in the sperm quality of mice administered ethanol.
Materials and Methods: Fifty-four BALB/c mice were categorized into nine groups (n = 6/each). The control group received a basal diet while the eight experimental groups received ethanol 10%; ethanol 20%; vit. E 100 mg; vit. E 200 mg; ethanol 10% + vit. E 100 mg; ethanol 10% + vit. E 200 mg; ethanol 20% + vit. E 100 mg; ethanol 20% + vit. E 200 mg. After 35 days, the sperm parameters and sperm chromatin were assessed.
Results: The results demonstrated a significant reduction in the motility rate, normal morphology rate, viability rate, increase in abnormal DNA structure and packaging (TB staining), and DNA damage (TUNEL) in ethanol consumer groups. In addition, the findings showed a significant increase in the aforementioned parameters in ethanol- and vit. E-consumer groups compared to the ethanol-only consumer groups. The ethanol group received 20% of the most damage among the groups. The group receiving vit. E 100 mg and those receiving ethanol 10% + vit. E 200 mg gained the highest benefit among the groups.
Conclusion: Sperm forward progressive motility, normal morphology rate, and viability decreased in the ethanol groups. Also, the rates of spermatozoa with abnormal DNA structure and DNA fragmentation increased in the ethanol groups. Our findings revealed that the co-administration of vit. E and ethanol can protect destructive changes in DNA structure and damage.
