en بررسی ویژگی های بیولوژیکی و فیزیولوژیکی سلول های کومولوس انسانی در شرایط آزمایشگاهی: یک مطالعه آزمایشگاهی Reproductive Biology Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">سلول&shy;های کومولوس به عنوان سلول&shy;های پرستار تخمک، یک کنام مناسب را شامل عوامل رشد و تداخل سلولی مورد نیاز برای بلوغ تخمک را ایجاد می&shy;کنند. بنابراین، این سلول&shy;ها ممکن است به عنوان کنامی طبیعی در مطالعات آزمایشگاهی تکوین سلول&shy;های زایا فراهم کنند. سلول</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">‌</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">‌های کومولوس می&shy;تواند به ما برای درک بهتر علل نازایی در زنان کمک کند که در نهایت می&shy;تواند به درمان ناباروری در موارد واکنش ضعیف به دارو درمان کند.</span></span><strong><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از این مطالعه، جداسازی، کشت و بررسی ویژگی&shy;های بیولوژیکی سلول&shy;های کومولوس انسان بود.</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در مطالعه حاضر، سلول&shy;های کومولوس</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">جداسازی و کشت داده شدند و توسط </span></span><span dir="LTR"><span style="font-size:10.0pt;">RT-PCR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با ژن</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های اختصاصی نظیر </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">FOXL2</span></span></em><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CYP19A1</span></span></em><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">FSHR</span></span></em><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">AMHR</span></span></em><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">LHR</span></span></em> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">شناسایی شدند. حضور </span></span><span dir="LTR"><span style="font-size:10.0pt;">vimentin</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، پروتئین ساختاری، با رنگ</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آمیزی ایمونوفلورسانس مورد بررسی قرار گرفت. علاوه بر این، سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">AMH</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و ترشح پروژسترون توسط سلول</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های کومولوس توسط </span></span><span dir="LTR"><span style="font-size:10.0pt;">ELISA</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بعد از 2، 4، 12، 24 و 48 ساعت در کشت اندازه</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گیری شد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">سلول</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های کومولوس انسانی در یک کشت چسبنده ژن</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها و مارکرهای اختصاصی را بیان می&shy;‌کنند، بعلاوه، </span></span><span dir="LTR"><span style="font-size:10.0pt;">AMH</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و پروژسترون را در محیط ترشح می‌کنند.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">سلول&shy;های کومولوس در شرایط کشت چسبنده، </span></span><span dir="LTR"><span style="font-size:10.0pt;">AMH</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و پروژسترون ترشح می</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کنند که ممکن است در بلوغ تخمک در شرایط آزمایشگاهی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و تکوین آزمایشگاهی سلول</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های زایا (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IVG</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) کاربرد داشته باشند.</span></span> <div><strong>Background: </strong>Cumulus cells, as oocyte nurse cells, provide a suitable microenvironment with growth factors and cellular interactions required for oocyte maturation. Thus, these cells may serve as a natural niche for <em>in vitro</em> studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.<br> <strong>Objective:</strong> The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.<br> <strong>Materials and Methods: </strong>In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including <em>FOXL2</em>, <em>CYP19A1</em>, <em>FSHR</em>, <em>AMHR</em>, and <em>LHR. </em>The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.<br> <strong>Results: </strong>In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.<br> <strong>Conclusion:</strong> Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for <em>in vitro</em> maturation (IVM) and <em>in vitro</em> gametogenesis (IVG) studies.<br> &nbsp;</div> سلول های کومولوس, محیط شرطی شده, IVM, IVG, کنام Cumulus cells, Conditioned medium, In vitro maturation, In vitro gametogenesis, Niche 1 10 http://ijrm.ir/browse.php?a_code=A-10-588-1&slc_lang=en&sid=1 Somayyeh Sadat Tahajjodi s.tahajjodi@gmail.com 0000-0001-7386-3753 Yes Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ehsan Farashahi Yazd ehsanfarashahi@gmail.com 0000-0003-1122-0626 No Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Azam Agha-Rahimi 63rahimi@gmail.com 0000-0002-7579-6192 No Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Reza Aflatoonian r.aflatoonian@gmail.com 0000-0001-8329-1445 No Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Mohammad Ali Khalili khalili59@hotmail.com 0000-0001-9940-749X No Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mahnaz Mohammadi mahnaz.moh65@gmail.com 0000-0001-5676-397X No Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Behrouz Aflatoonian b.aflatoonian@ssu.ac.ir 0000-0002-7250-9813 No Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.