International Journal of Reproductive BioMedicine International Journal of Reproductive BioMedicine IJRM Medical Sciences http://ijrm.ir 1 admin 2476-4108 2476-3772 10.29252/ijrm en jalali 1399 10 1 gregorian 2021 1 1 19 1 online 1 fulltext
en مهار قابل برگشت کنترل شده اسپرم به عنوان یک ضدحاملگی داخل توبولی و قابل برگشت در رت های ماده: یک مطالعه تجربی Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study Reproductive Biology Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مهار قابل برگشت کنترل شده </span></span><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">اسپرم یک روش موثر ضد حاملگی داخل وازال مردان است.</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">یک مطالعه تجربی جهت بررسی جنبه&shy;های مختلف</span></span><em><span style="font-size:10.0pt;"> <sup>&reg;</sup></span></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به طریقه غیر</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">تهاجمی و تک</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مرحله</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ای در جنس مونث انجام شد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه تجربی 60 رت ماده نژاد آلبینو ویستار با وزن 150 تا 155 گرم و با سن 3 تا 4 ماه به 4 گروه تقسیم شدند: گروه 1: کنترل شم، گروه 2: انسداد توبال با </span></span><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به مدت 90 روز، گروه 3: انسداد توبال با </span></span><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به مدت 90 روز و سپس برگشت با دی&shy;متیل&shy;سولفکسید و گروه 4: انسداد توبال با </span></span><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به مدت 90 روز و سپس برگشت با 5% </span></span><span dir="LTR"><span style="font-size:10.0pt;">NaHCO3</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">. حیوانات تحت انسداد دوطرفه توبال با </span></span><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و برگشت با </span></span><span dir="LTR"><span style="font-size:10.0pt;">DMSO</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">NaHCO3</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> قرار گرفتند. سیکل </span></span><span dir="LTR"><span style="font-size:10.0pt;">estrous</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">باروری و بافت</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">شناسی لوله</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های فالوپ بررسی شدند.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">گروه 1 میزان باروری 100% را در تمام جفت</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">گیری</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ها نشان دادند. حیوانات مطالعات تجربی جفت&shy;گیری مثبت را نشان دادند اما میزان باروری بعد از 30 و 60 و 90 روز پس از انسداد توبال 0% بود. به هر حال بعد از برگشت میزان باروری به طور پیوسته به میزان طبیعی در گروه&shy;های 3 (50% در 45 روز، 80% در 105 روز و 100% در 150 و 195 روز) و 4 (70% در 45 روز و 105 روز و 100% در 150 و 195 روز) افزایش یافت. در گروه 2 لایه&shy;های پوششی داخلی به هم ریخته و لوله&shy;های پرشده از ایمپلنت </span></span><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ائوزینه مشاهده شد. در گروه&shy;های برگشت داده شده 3 و 4 بازسازی کامل ساختمان بافت&shy;شناسی سلولی مشاهده شد. سیکل استروس منظم در تمام گروه&shy;های تجربی مشاهده شد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><em><sup><span style="font-size:10.0pt;">&reg;</span></sup></em><span dir="LTR"><span style="font-size:10.0pt;">RISUG</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">یک روش موثر تک مرحله</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ای ضد حاملگی داخل توبولی برگشت</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">پذیر در رت ماده است</span></span><br> &nbsp;<br> &nbsp; <div style="text-align: justify;"><strong>Background: </strong>Reversible inhibition of sperm under guidance (&ldquo;RISUG<sup>&reg;</sup>&rdquo;) is a promising intravasal male contraceptive.<br> <strong>Objective:</strong> An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUG<sup>&reg; </sup>in females.<br> <strong>Materials and Methods</strong>: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUG<sup>&reg;</sup> for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUG<sup>&reg;</sup> for 90 days and reversal with 5% NaHCO<sub>3</sub>. Animals were subjected to bilateral fallopian tube occlusion with RISUG<sup>&reg;</sup> and reversal with DMSO and NaHCO<sub>3</sub>. The estrous cycle, fertility and histology of fallopian tube were evaluated.<br> <strong>Results:</strong> Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUG<sup>&reg; </sup>implant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups.<br> <strong>Conclusion: </strong>RISUG<sup>&reg;</sup> is suitable for single intervention, intratubular, reversible contraception in female rats.<br> &nbsp;</div> پیشگیری از بارداری, لوله فالوپ, انسداد لوله,®RISUG, برگشت پذیر. Contraception, Fallopian tube, Tubal occlusion, RISUG®, Reversible. 47 56 http://ijrm.ir/browse.php?a_code=A-10-959-1&slc_lang=en&sid=1 Abdul Salam Ansari abdulsansari@yahoo.com 0000-0001-5244-5069 No Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India. Kiran Sevliya kiransevliya@gmail.com 0000-0003-4147-458X No Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India. Ayesha Badar: badrayesha@gmail.com 0000-0003-3549-1754 Yes Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India. Nirmal Kumar Lohiya lohiyank@gmail.com 0000-0002-3872-5803 No Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India.