International Journal of Reproductive BioMedicine International Journal of Reproductive BioMedicine IJRM Medical Sciences http://ijrm.ir 1 admin 2476-4108 2476-3772 10.29252/ijrm en jalali 1399 7 1 gregorian 2020 10 1 18 10 online 1 fulltext
en برهمکنش اسپرم با آندومتر می تواند ژن های موجود در مسیر پذیرش آندومتر در موش را تنظیم کند: یک مطالعه تجربی Interaction of sperm with endometrium can regulate genes involved in endometrial receptivity pathway in mice: An experimental study Reproductive Biology Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">زمینه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بسیاری از محققان لانه&shy;گزینی و پذیرش آندومتر را موضوعاتی مهم در علم تولیدمثل می&shy;دانند. اگرچه آزمایش&shy;های متعدد انجام شده و نتایج آنها ارزیابی شده است، با این حال، هنوز هم شواهدی تأیید شده در مورد عوامل مرتبط و نقش اسپرم در میزان پذیرش آندومتر وجود ندارد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف از این مطالعه بررسی اثر تعامل اسپرم با آندومتر روی تنظیم ژن&shy;های درگیر در مسیر پذیرش آندومتر است.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش&shy; ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه تجربی، 10 موش نر و 30 موش ماده </span></span><span dir="LTR"><span style="font-size:10.0pt;">NMRI</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">گنجانده شدند و نیمی از موش&shy;های نر تحت وازکتومی قرار گرفتند. موش&shy;های مورد مطالعه به دو گروه تقسیم شدند: گروه 1 (مورد) شامل 15 موش ماده جفتگیری شده با 5 موش نر وازکتومی نشده، در حالی که گروه 2 (شاهد) شامل 15 موش ماده جفتگیری شده با 5 نر وازکتومی شده بود. موش&shy;های گروه مورد کشته شدند و پس از 36 ساعت مورد ارزیابی قرار گرفتند و بافت آندومتر استخراج و برای ادامه مطالعه، در</span></span><span dir="LTR"><span style="font-size:10.0pt;">&ordm;C</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">80 نگهداری شد. بیان ژن&shy;های مسیر پذیرش آندومتر، از جمله </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">VEGF</span></span></em><span style="font-size:10.0pt;">،</span><em><span dir="LTR"><span style="font-size:10.0pt;">HBEGF</span></span></em> <span style="font-size:10.0pt;">،</span><em><span dir="LTR"><span style="font-size:10.0pt;">FGF2</span></span></em> <span style="font-size:10.0pt;">،</span><em><span dir="LTR"><span style="font-size:10.0pt;">EGF</span></span></em> <span style="font-size:10.0pt;">،</span><em><span dir="LTR"><span style="font-size:10.0pt;">LIF</span></span></em> <span style="font-size:10.0pt;">،</span><em><span dir="LTR"><span style="font-size:10.0pt;">LIFR</span></span></em> <span style="font-size:10.0pt;">، </span><em><span dir="LTR"><span style="font-size:9.5pt;">HOXA10</span></span></em><span style="font-size:10.0pt;">،</span><em><span dir="LTR"><span style="font-size:9.5pt;">MUC1</span></span></em> <span style="font-size:10.0pt;">،</span> <em><span dir="LTR"><span style="font-size:10.0pt;">PGR</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CSF</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، در هر دو گروه مورد بررسی قرار گرفت. برای آنالیز آماری از آزمون </span></span><span dir="LTR"><span style="font-size:10.0pt;">independent samples test</span></span> <span dir="LTR"><span style="font-size:10.0pt;">(Mean &plusmn; SD)</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> استفاده شد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">mRNA</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> ژن (045/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">)</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">LIF</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، (040/0</span></span><span style="font-size:10.0pt;"> = </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">)</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">LIFR</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، (032/0</span></span><span style="font-size:10.0pt;"> = </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">)</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">MUC1</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، (022/0</span></span><span style="font-size:10.0pt;"> =</span><span dir="LTR"><span style="font-size:10.0pt;">p </span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">VEGF</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، (035/0</span></span><span style="font-size:10.0pt;"> = </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">EFG</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;(040/0</span></span><span style="font-size:10.0pt;"> = </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">)</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">FGF2</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">&nbsp;به طور معنی&shy;داری در گروه مورد در مقایسه با کنترل بالا بوده است.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه گیری: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در نهایت، سمینال پلاسما بر روی بیان ژن&shy;های درگیر در مسیر لانه&shy;گزینی موفق مانند </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">LIF</span></span></em><span dir="LTR"><span style="font-size:10.0pt;">, <em>LIFR</em>, <em>MUC1</em>, <em>VEGF</em>, <em>EGF</em>, <em>FGF2</em></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> موثر است.</span></span> <strong>Background: </strong>Many researchers consider implantation and endometrial receptivity as pertinent issues in reproductive science by. Although, several experiments have been performed and their results evaluated, yet there is no confirmed evidence about the related factors and the role of sperm in endometrial receptivity.<br> <strong>Objective: </strong>To investigate the effect of the sperm-endometrium interaction in regulating genes involved in the endometrial receptivity pathway.<br> <strong>Materials and Methods:</strong> In this experimental study, 10 male and 30 female NMRI mice were included, and half of the male cases were vasectomized. The subjects were divided into two groups as follows; group 1 (case) comprised of 15 females mated with 5 non-vasectomized male mice, while group 2 (control) consisted of 15 females mated with 5 vasectomized males. Cases were sacrificed and assessed after 36 hr and the endometrial tissue was extracted and kept at -80&ordm;C until the next use. The expression of the endometrial receptivity pathway genes, including <em>VEGF, HBEGF, FGF2, EGF, LIF, LIFR, HOXA10, MUC1, PGR, </em>and<em> CSF,</em> was examined in both groups. For statistical analysis, an independent samples test (Mean &plusmn; SD) was used.<br> <strong>Results:</strong> The mRNA levels of <em>LIF </em><em>(</em>p = 0.045)<em>, LIFR</em> (p = 0.040)<em>, MUC1</em> (p = 0.032)<em>, VEGF </em>(p = 0.022)<em>, EFG </em>(p = 0.035)<em>, and FGF2</em> (p = 0.040) were <em>significantly upregulated</em> in the case group compared with the control group.<br> <strong>Conclusion:</strong> Finally, seminal plasma was observed to be effective in expressing the involved genes in the successful implantation pathway, including <em>LIF, LIFR, MUC1, VEGF, EGF, </em>and<em> FGF2</em>.<br> &nbsp; آندومتر پذیرنده, اسپرم, بیان ژن, موش. Endometrial receptivity, Sperm, Gene expression, Mice 815 824 http://ijrm.ir/browse.php?a_code=A-10-972-1&slc_lang=en&sid=1 Marziyeh Ajdary maa.biology92@gmail.com 0000-0002-4154-7788 No Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Zahra Zandieh zandie.dvm@gmail.com 0000-0002-7720-9465 No Department of Anatomical Science, Iran University of Medical Sciences, Tehran, Iran. Fatemeh Sadat Amjadi amjadi.bh@gmail.com 00-0003-4902-388X No Department of Anatomical Science, Iran University of Medical Sciences, Tehran, Iran. Fariborz Keyhanfar keyhanfar.f@iums.ac.ir 0000-0001-8312-9739 No Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mehdi Mehdizadeh mehdizadeh.m@iums.ac.ir 0000-0002-9268-7318 No Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Department of Anatomical Science, Iran University of Medical Sciences, Tehran, Iran. Reza Aflatoonian r.aflatoonian@gmail.com 0000-0001-8329-1445 Yes Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.