International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1399
6
1
gregorian
2020
9
1
18
9
online
1
fulltext
en
روی با کاهش بیان ژن کاسپاز 3 در رده سلولی لایدیگ موشی، آپوپتوز ناشی از اکستازی را کاهش میدهد
Zinc attenuates ecstasy-induced apoptosis through downregulation of caspase-3 in cultured TM3 cells: An experimental study
Reproductive Biology
Original Article
Original Article
<strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">3 و ۴ متیلن دی اکسی متا آمفتامین (</span></span><span dir="LTR"><span style="font-size:10.0pt;">MDMA</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">؛ اکستازی) بطور معمول به عنوان معروف</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ترین ترکیب مشتق شده از آمفتامین شناخته شده است. اثر اکستازی بر روی سلول</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های لایدیگ و بافت بیضه به طور گسترده</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ای مورد بررسی قرار نگرفته است. حداقل از نظر آپوپتوز در سلول­های لایدیگ، گزارشی وجود ندارد. </span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br>
<strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">این مطالعه با هدف ارزیابی تأثیر روی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">Zn</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) بر آپوپتوز ناشی از اکستازی و بیان ژن </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">caspase-3</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> در رده سلول لیدیگ (</span></span><span dir="LTR"><span style="font-size:10.0pt;">TM3</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) طراحی شده است. </span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br>
<strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش­ ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">سلول­های لایدیگ با اکستازی،</span></span><span dir="LTR"><span style="font-size:10.0pt;">Zn </span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و اکستازی پیش­تیمار با </span></span><span dir="LTR"><span style="font-size:10.0pt;">Zn</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">تیمار گردید و اثر آنها بر حیات سلول­های </span></span><span dir="LTR"><span style="font-size:10.0pt;">TM3</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بوسیله تست </span></span><span dir="LTR"><span style="font-size:10.0pt;">MTT</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ارزیابی شد. همچنین، سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">mRNA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کاسپاز 3 بوسیله </span></span><span dir="LTR"><span style="font-size:10.0pt;">real-time PCR</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> ارزیابی گردید. </span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br>
<strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">میانگین سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">mRNA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کاسپاز 3 (چند برابری بیان ژن) از لحاظ آماری بطور معنی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">داری با افزودن اکستازی با غلظت 5 میلی­مولار افزایش یافت. </span></span><span dir="LTR"><span style="font-size:10.0pt;">Zn</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">با غلظت 8 میکرومولار آپوپتوز را از طریق کاهش بیان ژن کاسپاز 3 در گروه</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های مورد مطالعه کاهش داد.</span></span><br>
<strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه­ گیری: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">این داده­ها یک مکانیسم مولکولی برای تشدید آپوپتوز ناشی از اکستازی بوسیله افزایش بیان ژن کاسپاز 3 در سلول­های </span></span><span dir="LTR"><span style="font-size:10.0pt;">TM3</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">پیشنهاد می­کند. و همچنین، پیش تیمار با </span></span><span dir="LTR"><span style="font-size:10.0pt;">Zn</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">آپوپتوز را از طریق کاهش بیان ژن کاسپاز 3 در سلول­های </span></span><span dir="LTR"><span style="font-size:10.0pt;">TM3</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کاهش می­دهد. </span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br>
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<strong><em><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:10.0pt;"></span></span></span></em></strong>
<strong>Background:</strong> 3, 4-Methylenedioxymethamphetamine (MDMA) is commonly known as the most famous amphetamine derivative.<br>
<strong>Objective:</strong> To evaluate the influence of zinc on MDMA-induced apoptosis and caspase- 3 gene expression in Leydig cell line (TM3).<br>
<strong>Materials and Methods: </strong>Leydig cells were studied in differenet treatment groups regarding MDMA (0, 0.5, 1, 3, 5 mM) and zinc (0, 4, 8, 16, 32 μM). By the way, the effective concentration was determined to be 5 mM for MDMA and 8 μM for zinc. Then, TM3 cells were cultured in free medium as control (group I), medium containing MDMA (5 mM) (group II), zinc (8 µM) (group III), and zinc (8 µM) prior to MDMA (5 mM) (group IV) as well as in an untreated group (control). Cell viability was assessed at different times after cell culture by MTT assay. The mRNA expression level of caspase-3 was analyzed using real-time quantitative polymerase chain reaction.<br>
<strong>Results:</strong> The cellular viability was significantly reduced in TM3 cells after 24 hr and 48 hr exposure time regarding different concentrations of MDMA as well as high concentration of zinc (16 and 32 μM). Cell viability was increased in the group that received zinc (8 µM) before addition of MDMA (5 mM) compared to the control and MDMA groups. The mean ± SE of fold was 22.40 ± 7.5, 0.06 ± 0.02, and 0.009 ± 0.003 in MDMA, zinc, and zinc + MDMA groups, respectively. The mean of caspase-3 mRNA level was significantly increased in the MDMA-treated group (5 mM), while the relative expression of caspase-3 gene was significantly decreased in the zinc (8 µM) + MDMA (5 mM) group compared with the MDMA (5 mM) group (p = 0.001).<br>
<strong>Conclusion:</strong> Dietary intake of zinc has a protective effect against MDMA consumption in mouse.<br>
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روی, اکستازی, آپوپتوز, سلول لایدیگ.
Zinc, MDMA, Apoptosis, TM3 cells.
777
784
http://ijrm.ir/browse.php?a_code=A-10-1025-1&slc_lang=en&sid=1
Marziyeh
Lozeie
marlin_ml@yahoo.com