en اثرات تجربی هایپرتیروئیدیسم بر بیان ژن های CATSPER1 و CATSPER2 در توبول های سمینیفروس موش نژاد BALB/C: مطالعه تجربی Reproductive Anatomy Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کانال&shy;های </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER1</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و </span></span>&nbsp;<em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER2</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نقش مهمی در تحرک اسپرم دارند. اثرات پرکاری تیروئید براین کانال</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ها در عملکرد اسپرم مورد بررسی قرارگرفت.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مطالعه اخیر به منظور بررسی اثرات پرکاری تیروئید بر بیان ژن&shy;ها و پروتئین&shy;های </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER1</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER2</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در لوله&shy;های سمینفروس موش انجام گرفته است.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها:</span></span></strong> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">این تحقیق برروی 20 موش نژاد</span></span> <span dir="LTR"><span style="font-size:10.0pt;">BALB/C</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">انجام شد که به دو گروه مساوی تجربی و کنترل تقسیم شدند</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">.</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> گروه تجربی 500 میلی&shy;گرم در لیتر محلول مایع لووتیروکسین (</span></span><span dir="LTR"><span style="font-size:10.0pt;">-L</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">تیروکسین) را به مدت دو ماه برای القاء پرکاری تیروئید دریافت کردند که با آزمایشات</span></span> <span dir="LTR"><span style="font-size:10.0pt;">radioimmunoassay</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مورد تایید قرارگرفت. از طرف دیگر گروه گنترل در خانه حیوانات تحت شرایط طبیعی نگهداری شدند. پس از بیهوشی حیوانات با کلروفرم، بیضه&shy;ها به منظور تغییرات ایمنوهیستوشیمیایی و مطالعات </span></span><span dir="LTR"><span style="font-size:10.0pt;">Real-time PCR</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> استخراج گردیدند.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بر اساس نتایج حاصله اختلاف معنی&shy;داری در بیان ژن&shy;های کت اسپری یک (45/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p= </span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) و دو (34/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p= </span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) در گروه&shy;های تجربی و کنترل مشاهده نشد، در ضمن اختلاف معنی&shy;داری در شدت رنگ&shy;پذیری ایمونوهیستوشیمی </span></span><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">(</span></span> <em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER1</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">17/0 </span></span><span dir="LTR"><span style="font-size:10.0pt;">p=</span></span><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER2</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">22/0 </span></span><span dir="LTR"><span style="font-size:10.0pt;">p=</span></span><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> گروه&shy;های تجربی در مقایسه با کنترل مشاهده نشد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">با توجه به نقش کلیدی </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">CATSPER</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> در ساختار مولکولی اسپرم، یافته&shy;های ما نشان داد که پرکاری تیروئید اثرات قابل توجهی برعملکرد این اجزا ندارد.</span></span><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"></span></span><br> &nbsp;<br> &nbsp; <strong>Background:</strong><em> CATSPER 1</em> (Cation Channel Sperm Associated 1) and <em>CATSPER2</em> channels have an important role in sperm motility. In this study, the effects of hyperthyroidism on <em>Catsper1</em> and <em>2</em> genes of seminiferous tubules in mice testes were investigated.<br> <strong>Objective: </strong>The present study was conducted to investigate the effect of hyperthyroidism on the expression of <em>CATSPER1</em> and <em>CATSPER</em><em>2</em> genes in the seminiferous tubules of mice.<br> <strong>Materials and Methods:</strong> This study was conducted on 20 BALB/C male mice divided into two groups - experimental and control. The experimental group was administered with 500 mg/l levothyroxine (L-thyroxine) liquid solution for two months for inducing hyperthyroidism, which was confirmed by radioimmunoassay. On the other hand, the control group was kept in animal houses under a normal condition. The implementation of real-time polymerase chain reaction and immunohistochemical studies was accomplished after the removal of the testes of the mice under anesthesia induced by chloroform.<br> <strong>Results: </strong>Results showed that there was no significant difference in <em>CATSPER1</em> (p = 0.45) and <em>CATSPER2</em> (p = 0.34) gene expression between groups. At the same time, the color intensity showed no significant enhancement in the hyperthyroidism group (<em>CATSPER1</em> p = 0.17 and <em>CATSPER2</em> p = 0.22) as compared to the control group.<br> <strong>Conclusion:</strong> Considering the key role of <em>CATSPER</em> in the molecular structure of the sperm, our findings showed that the hyperactivity of the thyroid gland has no significant effects on the function of these components. Therefore, it might be concluded that hyperthyroidism has no considerable effects on the seminiferous tubules. CATSPER1, CATSPER2, هایپرتیروئیدیسم, موش, اسپرم. CATSPER1, CATSPER2, Hyperthyroidism, Mice, Sperm. 597 604 http://ijrm.ir/browse.php?a_code=A-10-1028-1&slc_lang=en&sid=1 Saeed Sadeghi Sadeghis912@mums.ac.ir 0000-0002-4062-0170 No Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran. Mahdi Jalali JalaliM@mums.ac.ir 0000-0002-9983-3533 Yes Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran. Mohammad Reza Nikravesh Nikraveshmr@mums.ac.ir 0000-0002-5500-4610 No Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran. Mojtaba Sankian SankianM@mums.ac.ir 0000-0003-0602-6431 No Immunology Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran.