en افزایش بیان hsa-miR-27a-3p در مردان آزواسپرمی غیر انسدادی: یک مطالعه ی مورد-شاهدی Fertility & Infertility Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نقش کلیدی ژن </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">KDM3A</span></span></em><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و ژن‌های پایین دستی آن در باروری مردان در مدل</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های حیوانی ثابت شده ‌است. هم‌چنین کاهش بیان این ژن به طور آشکاری با فنوتیپ آزواسپرمی مردان در ارتباط است. بیان نابجای </span></span><span dir="LTR"><span style="font-size:10.0pt;">micro-RNA</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> ها می‌تواند سبب اخلال در اسپرماتوژنز شود و عمدتأ باعث ایجاد فنوتیپ&shy;های متغیری از ناباروری در مردان می‌شود.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه بیان </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">hsa-miR-27a-3p</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در افراد آزواسپرمی ارزیابی می</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">شود تا ارتباط آن با آزواسپرمی در مردان سنجیده شود.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش&shy; ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مطالعه‌ی حاضر بر روی 30 فرد مبتلا به آزواسپرمی صورت گرفت که از این تعداد، بر اساس آزمایش‌های صورت گرفته، 19 نفر مبتلا به آزواسپرمی غیر&shy;انسدادی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">NOA</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) و 11 نفر مبتلا به آزواسپرمی انسدادی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">OA</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) بودند. مطالعات گسترده‌ بیوانفورماتیکی صورت گرفت و در نهایت&nbsp;&nbsp;</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">hsa-miR-27a-3p</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">انتخاب شد. روش رونوشت&shy;برداری معکوس و واکنش کمی زنجیر پلیمراز </span></span><span dir="LTR"><span style="font-size:10.0pt;">Real-time polymerase chain reaction: RT-qPCR</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">استفاده شد و از بررسی‌های آماری برای مقایسه سطح بیان </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">has- miR- 27a-3p</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در افراد </span></span><span dir="LTR"><span style="font-size:10.0pt;">NOA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">OA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">استفاده شد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مطالعات بیوانفورماتیکی، </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">hsa-miR-27a-3p</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">را به دلیل توانایی اتصال به رونوشت ژن </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">KDM3A</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">معرفی کرد. بررسی بیان </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">hsa-miR-27a-3p</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کاندید شده، حاکی از بیان بیش از حد آن در مردان </span></span><span dir="LTR"><span style="font-size:10.0pt;">NOA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بود.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></span></strong><em><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">hsa-miR-27a-3p</span></span></em> <span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بیان بالاتری در مردان </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">NOA</span></span> <span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نسبت به افراد </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">OA</span></span> <span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به عنوان گروه کنترل، نشان داد. به عنوان یک نتیجه، بیان بیش از حد این </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">miRNA</span></span> <span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">می</span></span></span><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span><span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">تواند به طور مستقیم سبب کاهش بیان ژن </span></span></span><em><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">KDM3A</span></span></em> <span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">شده و به طور غیر</span></span></span><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span><span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مستقیم بر ژن‌های </span></span></span><em><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">TNP1</span></span></em>&nbsp;<span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span></span><em><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">PRM1</span></span></em> <span dir="RTL"><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">تأثیر گذارد. در نتیجه اسپرماتوژنز می&shy;تواند دچار اخلال شده و ناباروری در مردان را سبب شود.</span></span></span> <strong>Background: </strong>The role of <em>KDM3A</em> and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of <em>KDM3A</em> is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility.<br> <strong>Objective</strong><strong>:</strong> The aim of this study was to evaluate the expression level of <em>hsa-miR-27a-3p</em> in azoospermic men to reveal its possible association with infertility.<br> <strong>Materials and Methods:</strong> This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and <em>hsa-miR-27a-3p</em> was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RT-qPCR<strong>)</strong> method was used and statistical analysis was performed to compare the expression level of <em>hsa-miR-27a-3p</em> in both OA and NOA individuals.<br> <strong>Results:</strong> In silico analysis suggested <em>hsa-miR-27a-3p</em>, with its potential binding ability to target <em>KDM3A</em> transcripts. The expression analysis of candidate <em>hsa-miR-27a-3p</em> indicated its significant overexpression in NOA men.<br> <strong>Conclusion</strong><strong>:</strong> The <em>hsa-miR-27a-3p</em> was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly <em>KDM3A </em>and indirectly <em>TNP1</em> and <em>PRM1</em>. Therefore, spermatogenesis could be misled and male infertility could be developed. hsa-miR-27a-3p, ناباروری مردان, KDM3A. hsa-miR-27a-3p, Male infertility, KDM3A. 961 968 http://ijrm.ir/browse.php?a_code=A-10-948-1&slc_lang=en&sid=1 Hamid Norioun hamidnori1043@gmail.com 0000-0002-9637-1580 No Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. Majid Motovali-bashi mbashi@sci.ui.ac.ir 0000-0003-4380-0166 Yes Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. Seyed Morteza Javadirad sm.javadirad@sci.ui.ac.ir 0000-0002-2891-2293 No Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.