International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1398
3
1
gregorian
2019
6
1
17
6
online
1
fulltext
en
کلومیفن سیترات بیان CD98 آندومتر را در موشهای فاقد و دارای تخمدان کاهش می دهد
Clomiphene citrate impairs the endometrial
CD98 expression in ovariectomized and
non-ovariectomized rats: Role of HCG
Original Article
Original Article
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کلومیفن سیترات (</span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) یکی از داروهایی است که به­طور گسترده به عنوان محرک تخمک­گذاری استفاده می­شود، اما اثرات جانبی آن بر آندومتر باعث کاهش میزان بارداری می­شود. میزان بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در سلول­های آندومتر نیز در روند لانه­گزینی مهم است.</span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از انجام مطالعه، ارزیابی سطوح بیان ایمونوهیستوشیمی </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">آندومتر پس از تزریق </span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">با و بدون هورمون گونادوتروپین جفتی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در موش­های دارا و فاقد تخمدان.</span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش­ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">72 موش صحرایی نر نژاد ویستار (سن 12-14 هفته) به صورت تصادفی به دو گروه (36 تایی) تقسیم شدند: (الف) اوارکتومی و (ب) غیر اوارکتومی شده. هر گروه به شش زیرگروه تقسیم شدند: (1)</span></span><span dir="LTR"><span style="font-size:10.0pt;"> mg/kg</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">10</span></span><span dir="LTR"><span style="font-size:10.0pt;">CC </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، (2)</span></span> <span dir="LTR"><span style="font-size:10.0pt;">mg/kg</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">20</span></span><span dir="LTR"><span style="font-size:10.0pt;">CC </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، (3) </span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، (4) </span></span> <span dir="LTR"><span style="font-size:10.0pt;">mg/kg</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">10</span></span><span dir="LTR"><span style="font-size:10.0pt;">CC </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با </span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، (5) </span></span><span dir="LTR"><span style="font-size:10.0pt;">mg/kg</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;"> 20</span></span><span dir="LTR"><span style="font-size:10.0pt;">CC </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با </span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و (6) کنترل. زیرگروه­های آزمایشی به تنهایی یا به صورت ترکیبی یک دوز </span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">(روزانه، پنج روز) و </span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">(پس از آخرین تزریق </span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) دریافت کردند. رنگ</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آمیزی ایمونوهیستوشیمی جهت بررسی سطح بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بر روی بافت</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های آندومتر حاوی پارافین انجام شد.</span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">میزان بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">آندومتر در زیرگروه</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های دارای تخمدان به طور معنی</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">داری (001/0</span></span><span style="font-size:10.0pt;"><</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در مقایسه با زیرگروه­های اوارکتومی شده با شرایط مشابه بالاتر بود. همچنین در مقایسه با گروه شاهد کاهش معنی­داری در بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">وابسته به دوز کلومیفن سیترات (001/0</span></span><span style="font-size:10.0pt;">></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در زیرگروه­های اوارکتومی شده دیده شد. علاوه بر این، تزریق </span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">پس از درمان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">باعث بهبود نسبی در بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">گردید.</span></span><strong><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه­ گیری:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">با توجه به نتایج بدست آمده، </span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">آندومتر اختلال ایجاد می­کند و این اختلال با حذف تخمدان تشدید می­شود. همچنین، تزریق </span></span><span dir="LTR"><span style="font-size:10.0pt;">HCG</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">پس از درمان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">CC</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">می</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">­</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تواند کمی بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD98</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">را بهبود بخشد.</span></span>
<strong>Background:</strong> Clomiphene citrate (CC) is one of the widely used drugs as an ovulation stimulant, but its adverse effects on the endometrium results in lowering down the pregnancy rate. Endometrium CD98 is also important in the process of implantation.<br>
<strong>Objective: </strong>To evaluate the immunohistochemistry expression levels of endometrial CD98 following injection of CC with and without Human chorionic gonadotropin (HCG) in ovariectomized and non-ovariectomized rats.<br>
<strong>Materials and Methods:</strong> Seventy two (12-14 wk old) female Wistar rats were randomly divided into two groups (n = 36): (a) ovariectomized and (b) non-ovariectomized. Each group was further divided into six subgroups (n = 6/each): (1) CC 10 mg/kg, (2) CC 20mg/kg, (3) HCG, (4) CC 10 mg/kg with HCG, (5) CC 20 mg/kg with HCG, and (6) control. The experimental subgroups received a single dose of CC (daily, five days) and HCG<br>
(after the last injection of CC) alone or in combination. Immunohistochemistry staining was performed on paraffin-embedded endometrial tissues to evaluate the expression levels of CD98.<br>
<strong>Results: </strong>Animals undergoing ovariectomy presented a significantly lower expression level of endometrial CD98 (p < 0.001) when compared with non-ovariectomized in the same condition that groups were subdivided. There was also a dose-dependent reduction (p < 0.001) in the expression of CD98 in non-ovariectomized subgroups when compared with control group. In addition, injection of HCG following treatment with CC improved its expression.<br>
<strong>Conclusion: </strong>It was concluded that CC impairs CD98 expression in endometrium and this impairment is intensified with the removal of the ovary. Also, an injection of HCG following treatment with CC can slightly improve the expression of CD98.
آندومتر, کلومیفن سیترات,HCG , CD98.
Endometrium, Clomiphene citrate, Human chorionic gonadotropin, CD98.
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http://ijrm.ir/browse.php?a_code=A-10-31-100&slc_lang=en&sid=1
Behpour
Yousefi