en بررسی تأثیردیابت ملیتوس بر بیان ژن اینتگرین‌ها در آندومتر رت در بازه زمانی لانه گزینی جنین Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بیماری دیابت ملیتوس به&shy;شدت میزان بیان ژن اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها را در بافت</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها و سلول</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های مختلف بدن از جمله: مونوسیت</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها، سلول</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های آندوتلیال عروق،</span></span>&nbsp;&nbsp; <span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;سلول</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های گلومرولار کلیوی، شبکیه&shy;ی چشم و ...&nbsp; تحت تأثیر قرار می&shy;دهد. علاوه بر این دیابت و هایپرگلایسمی می&shy;تواند میزان موفقیت بارداری را در تکنیک&shy;های کمک باروری (</span></span><span dir="LTR"><span style="font-size:10.0pt;">ART</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و حتی در بارداری طبیعی تحت تاثیر قرار دهد. در یک سیکل جنسی طبیعی آندومتر تغییرات زیادی می&shy;کند و سوال این است که این بافت تحت شرایط دیابت چه تغییراتی به خود می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بیند؟</span></span><strong><span dir="LTR"><span style="font-size:11.0pt;"></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از این مطالعه بررسی بیان ژن اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های </span></span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">3</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">،</span></span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">v </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">&beta;1</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">&beta;3</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در آندومتر رت</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های مدل دیابتیک در زمان لانه</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گزینی جنین می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باشد.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش&shy;ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">28 رت ماده نژاد ویستار به 4 گروه: کنترل، دیابتیک، دیابتی درمان شده با متفورمین و دیابتی درمان شده با پیوگلیتازون تقسیم شدند. سپس در زمان لانه</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گزینی جنین (4 روز بعد از مشاهده پلاگ واژینال) با استفاده از تکنیک </span></span><span dir="LTR"><span style="font-size:10.0pt;">Real-time PCR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بیان ژن اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های</span></span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">3 </span></span><span style="font-size:10.0pt;">، </span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">4</span></span><span style="font-size:10.0pt;">،</span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">v </span></span><span style="font-size:10.0pt;">، </span><span dir="LTR"><span style="font-size:10.0pt;">&beta;1</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">&beta;3</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در آندومتر مورد مطالعه قرار گرفت.</span></span><strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">میزان بیان ژن زیر واحد</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها در رت</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های گروه دیابتیک در مقایسه با گروه کنترل به&shy;صورت معناداری افزایش یافته بود. درمان با پیوگلیتازون به&shy;صورت معناداری بیان ژن</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها را نسبت به گروه دیابتیک کاهش داد در حالیکه متفورمین باعث افزایش بیان اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های</span></span><span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">3</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;و</span></span> &nbsp;<span dir="LTR"><span style="font-size:10.0pt;">&alpha;</span></span><span dir="LTR"><span style="font-size:10.0pt;">4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">شد اما بیان دیگر اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها را کاهش داد.</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">دیابت به صورت معناداری میزان بیان ژن اینتگرین</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های مورد مطالعه را افزایش داد بنابراین می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تواند یکی از عوامل بالقوه در شکست لانه</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گزینی در نظر گرفته شود.</span></span><strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span></strong> <strong>Background:</strong> Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition?<br> <strong>Objective: </strong>The aim of the current study was to investigate the endometrial gene expression of &alpha;3, &alpha;4, &alpha;v, Itg &beta;1 and &beta;3 subunits in diabetic rat models at the time of embryo implantation.<br> <strong>Materials and Methods:</strong> Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Realtime PCR was performed to determine changes in the expression of Itg &alpha;3, &alpha;4, &alpha;v, &beta;1, and &beta;3 genes in rat&rsquo;s endometrium.<br> <strong>Results: </strong>The expression of all Itg subunits increased significantly in diabetic rats&rsquo; endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on &alpha;3 and &alpha;4 and elevated these two subunits gene expression.<br> <strong>Conclusion:</strong> Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure. دیابت, ایمپلنت ایمنی, انتگرئین, آندومتریوم. Diabetes mellitus, Embryo implantation, Integrins, Endometrium 395 404 http://ijrm.ir/browse.php?a_code=A-10-31-101&slc_lang=en&sid=1 Abbas Bakhteyari 0000-0002 – 6921 - 2613 No Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Yasaman Zarrin 0000 – 0002 – 3366 - 7683 No Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Parvaneh Nikpour 0000 – 0002 – 1784 - 6040 No Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Zeinab Sadat Hosseiny 0000 – 0002 – 7030 - 2798 No Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Fatemah Sadat Mostafavi 0000 – 0003 – 3649 - 4607 No Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Nahid Eskandari 0000 – 0003 – 1910 - 9356 No Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Mohammad Matinfar 0000 – 0003 – 4861 - 4477 No Department of Internal Medicine Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Roshanak Aboutorabi aboutorabi.r@gmail.com 0000-0003-1248-552X Yes Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.