en اثرات سودمند عصاره کاسنی (Cichorium intybus L.) بر وضعیت اکسیداتیو و پارامترهای تولیدمثلی در موش های صحرایی نر: یک مطالعه تجربی Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در سال&shy;های اخیر امروزه نگرانی&shy;های پیشرونده&shy;ای در خصوص کاهش تعداد اسپرم و بروز ناباروری در مردان وجود دارد. کاسنی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">Cichorium intybus L</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:10.0pt;">.</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) از جمله گیاهانی است که در طب سنتی ایران جهت محافظت از کبد و تصفیه خون بکار رفته و این عقیده وجود دارد که دارای خواص افزاینده باروری&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;می</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باشد.</span></span><strong><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مطالعه</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ای</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">دوز-</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">پاسخ</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">جهت</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">ارزیابی</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">اثرات عصاره الکلی برگ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> کاسنی (</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">C</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">.<em> intybus</em></span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بر </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">وزن اندام</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های تولیدمثلی، کیفیت اسپرم، سطوح سرمی هورمون</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">FSH</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:11.0pt;">, </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">LH</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و تستوسترون</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، لیپید پراکسیداسیون و آنزیم</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های آنتی</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">اکسیدان در موش</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های صحرایی</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">نر</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بالغ</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">نژاد ویستار</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">گرفت</span></span><span dir="LTR"><span style="font-size:11.0pt;">.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش&shy;ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در این مطالعه تجربی، موش</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های نر سالم به مدت 70 روز تحت درمان با مقادیر 100، 200 و 400 میلی</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گرم بر کیلوگرم در هر روز برگ</span> کاسنی (</span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">C</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">.<em> intybus</em></span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) از طریق گاواژ قرار گرفتند. </span></span><strong><span dir="LTR"><span style="font-size:11.0pt;"></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">افزایش معنی</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">داری در وزن بیضه و اپیدیدیم در موش</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های صحرایی دریافت&shy;کننده 200 میلی&shy;گرم بر کیلوگرم در هر روز عصاره کاسنی (</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">C</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">.<em> intybus</em></span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span> &nbsp;<span style="font-family:b mitra;"><span style="font-size:11.0pt;">مشاهده شد. غلظت اسپرم و درصد اسپرم&shy;های واجد مورفولوژی نرمال در موش&shy;های صحرایی دریافت&shy;کننده عصاره کاسنی (</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">C</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">.<em> intybus</em></span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) بصورت وابسته به دوز افزایش نشان داد. عصاره کاسنی (</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">C</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">.<em> intybus</em></span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span> <em><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بطور </span></span></em><span style="font-family:b mitra;"><span style="font-size:11.0pt;">معنی&shy;داری موجب کاهش سطوح مالون دی&shy;آلدئید و نیز افزایش معنی&shy;دار فعالیت آنزیم&shy;های سوپراکسید دسموتاز و گلوتاتیون پراکسیداز در بافت بیضه موش&shy;های صحرایی گردید.</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه &shy;گیری:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج بدست آمده نشان داد که عصاره برگ کاسنی (</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">C</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">.<em> intybus</em></span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">باعث بهبود پارامترهای تولیدمثلی موش&shy;های صحرایی نر </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">شده که این مسأله می&shy;تواند بدلیل ویژگی&shy;های آنتی&shy;اکسیدانی و آندروژنیک این گیاه باشد.</span></span><strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span></strong> <strong>Background: </strong>During recent years, increasing concern has been raised about the declining sperm count and human male infertility. Cichorium intybus L. (C. intybus) has traditionally been used in Iranian folk medicine as hepato protective and blood purifier and for its presumed fertility-enhancing properties.<br> <strong>Objective: </strong>A dose-response study was performed to determine the effect of C. intybus ethanolic leave extract on the reproductive parameters in adult Wistar male rats.<br> <strong>Materials and Methods: </strong>In this experimental study, 40 healthy adult male Wistar rats (8 wk old, 200-210 gr body weight) were randomly divided (n=10/each) as control and groups treated with 50, 100, and 200 mg/kg/day of C. intybus extract via gavage for 70 days. Serum hormonal assay, epididymal sperm evaluation, and analysis of morphometrical parameters, antioxidant enzymes, and lipid peroxidation levels of testis were done in each experimental group.<br> <strong>Results: </strong>Weights of testis and epididymis increase significantly in male rats treated with 200 mg/kg C. intybus extract. Sperm density and percent of morphologically normal sperm were significantly increased in a dose-related manner with C. intybus treatment.Serum testosterone was higher at 100 and 200 mg/kg C. intybus extracttreated groups. C. intybus significantly reduced malondialdehyde levels and also increased superoxide dismutase and glutathione peroxidase activity in testicular tissue of rats.<br> <strong>Conclusion:</strong> It is concluded that C. intybus leave extract improves reproductive parameters in male rats which might be a consequence of both its antioxidant and androgenic properties. کاسنی (Cichorium intybus L.), تولیدمثل, اسپرماتوژنز, استرس اکسیداتیو. Spermatogenesis, Oxidativestress, Rat, Cichorium intybus L. 425 434 http://ijrm.ir/browse.php?a_code=A-10-31-104&slc_lang=en&sid=1 Mehran Dorostghoal dorostghoal@gmail.com 0000-0003-1182-9685 Yes Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Seyyed Mansour Seyyednejad 0000-0001-6355-5941 No Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Marzieh Noroozi Tabrizi Nejad 0000-0003-3501-1909 No Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.