en بررسی تأثیرهپاتیت ویروسی B و هپاتیت ویروسی C روی پارامترهای اسپرم در مردان نابارور Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هپاتیت&shy;های ویروسی یکی از مشکلات مهم بهداشتی هستند که تأثیر به</span></span><span style="font-size:11.0pt;">&shy;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">سزایی روی مقوله سلامت دارند. به&shy;نظر می&shy;رسد که هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">تأثیر منفی روی کیفیت اسپرم و درصد ناباروری در مردان داشته باشد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف ما از این مطالعه ارزیابی تأثیر هپاتت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">روی کیفیت اسپرم در مردان نابارور ایرانی مراجعه</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کننده به مؤسسه&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;زیست&shy;پزشکی طب تولیدمثل رویان از سال 1382 لغایت 1393 می&shy;باشد.</span></span><strong><span dir="LTR"><span style="font-size:11.0pt;"></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش&shy;ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">این مطالعه مورد- شاهد گذشته&shy;نگر شامل 112 مرد نابارور هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مثبت و 47 مرد نابارور هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مثبت به عنوان گروه مورد و 112 مرد نابارور هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">منفی به عنوان گروه شاهد می</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باشد. ارزیابی آنالیز نمونه&shy;های منی و پارامتر&shy;های ویروسی در آزمایشگاه مرکزی موسسه رویان با روش و وسایل یکسان انجام شد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در این مطالعه تعداد اسپرم در مردان نابارور هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مثبت به طور قابل توجهی کمتر از گروه شاهد بود،&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span><span dir="LTR"><span style="font-size:10.0pt;">mean of total sperm count]</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ،001/0</span></span><span dir="LTR"><span style="font-size:10.0pt;"> p<</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">،25/151</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">27/166 ،18/141</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">22/118 ،59/118</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">95/100</span></span><span dir="LTR"><span style="font-size:10.0pt;">[</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، حرکت اسپرم در مردان نابارور هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مثبت به طور قابل توجهی در مقایسه با گروه شاهد کاهش داشت. </span></span><span dir="LTR"><span style="font-size:10.0pt;">respectively mean of sperm motility]</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ،007/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p<</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ،37/23</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">87/40 ،72/28</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">09/31، 88/25</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">97/30</span></span><span dir="LTR"><span style="font-size:10.0pt;">[</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، درصد اسپرم با مورفولوژی نرمال در گروه کنترل به&shy;طور قابل توجهی در مقایسه با بیماران هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مثبت بیشتر</span></span> <span dir="LTR"><span style="font-size:10.0pt;">mean of normal sperm morphology]</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ،015/0</span></span><span dir="LTR"><span style="font-size:10.0pt;"> p<</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">،15/3</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">51/4 ،83/3</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">70/3 ،27/3</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">23/3</span></span><span dir="LTR"><span style="font-size:10.0pt;">[</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، بود. گرچه کاهش قابل توجهی در زمان گدازش در گروه مورد وجود داشت اما ویسکوزیته، حجم منی و </span></span><span dir="LTR"><span style="font-size:10.0pt;">PH</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">نمونه&shy;های منی در هر دو گروه مورد و شاهد یکسان بود.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه &shy;گیری:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج مطالعه ما نشان می&shy;دهد که هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و هپاتیت ویروسی </span></span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">به کیفیت ضعیف اسپرم ارتباط دارد.</span></span><br> &nbsp; <strong>Abstract</strong><br> <strong>Background: </strong>Viral hepatitis is one of the health problems which have the effects on the health issues. It seems that hepatitis B virus (HBV) and hepatitis C virus (HCV) infection have negative impacts on the semen quality and male infertility rate.<br> <strong>Objective: </strong>In this study, we aimed to evaluate the effects of HBV and HCV on sperm quality among Iranian infertile men referred to Royan Institute Reproductive Biomedicine Research Center between 2003 and 2014.<br> <strong>Material and Methods:</strong> This retrospective case-control study included 112 HBV positive infertile men and 47 HCV positive infertile men as case group and 112 HBV negative and HCV negative matched infertile men as a control group. All semen analysis and viral parameters assessment was performed in the central laboratory with the same method and instruments.<br> <strong>Results:</strong> Sperm count among infertile men with HBV and HCV infection was significantly lower than control group [the mean of the total sperm count 100.95 &plusmn; 118.59, 118.22 &plusmn; 141.18, 166.27 &plusmn; 151.25 (p < 0.001)]. Sperm motility was significantly decreased in HBV and HCV positive men in comparison to the control group [30.97 &plusmn; 25.88, 31.09 &plusmn; 28.72, 40.87 &plusmn; 23.37, respectively (p < 0.007)]. The percentage of normal sperm morphology was significantly higher in control group in comparison to HBV and HCV infected group [the mean of the normal semen morphology 3.23 &plusmn; 3.27, 3.70 &plusmn; 3.83, 4.51 &plusmn; 3.15 p< 0.015]. Although there is a significant decline in liquefaction time in the case group but the viscosity, semen volume, and PH of semen samples were similar in the both case and control groups.<br> <strong>Conclusion: </strong>Our results suggest that HBV and HCV infection are associated with poor sperm quality. هپاتیت ویروسی B, هپاتیت ویروسی C, مردان, ناباروری, اسپرم. Hepatitis B virus, Hepatitis C virus, Male, Infertility, Sperm. 551 556 http://ijrm.ir/browse.php?a_code=A-10-1-646&slc_lang=en&sid=1 Sana karamolahi 0000-0002-0801-0989 No Tehran Medical Sciences, Branch Islamic Azad University, Tehran, Iran. Reza Salman Yazdi 0000-0003-3435-7641 No Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Mehrangiz Zangeneh zangeneh4@yahoo.com 0000-0001-7673-4287 Yes Department of Infectious Diseases, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Mahin Jamshidi Makiani 0000-0001-9685-0920 No Antimicrobial Resistance Research Center, Institute of Immunology & Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. Behnam farhoodi 0000-0001-7902-4539 No Department of Infectious Diseases, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Mohammad Ali Sedighi Gilani 0000-0001-3435-7641 No Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran