en آیا رسوراتول روی پارامترهای اسپرم آماده سازی شده و کیفیت کروماتین افراد نرموزوسپرمی و آستنوزوسپرمی قبل و بعد از فریز تاثیر دارد؟ Reproductive Andrology Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مطالعات قبلی اثر رسوراتول روی نمونه سیمن را ارزیابی کردند.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه به بررسی اثر رسوراتول روی پارامترهای اسپرم آماده&shy;سازی شده و کیفیت کروماتین افراد نرموزوسپرمی و آستنوزوسپرمی قبل و بعد از فریز پرداخته&shy;ایم.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها:</span></span></strong> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">تعداد 10 نمونه نرموزوسپرمی و 10 نمونه آستنوزوسپرمی با روش </span></span><span dir="LTR"><span style="font-size:10.0pt;">swim up</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> آماده شد. سپس هر نمونه را به دو گروه کنترل و درمان (اضافه کردن 30 میکرومول/لیتر رسوراتول) تقسیم کردیم و پارامترها و کیفیت کروماتین قبل و بعد فریز ارزیابی شد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج نشان داد که تحرک و حیات اسپرم&shy;ها بطور قابل ملاحظه&shy;ای قبل و بعد فریز در دو گروه متفاوت بود (001/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p<</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">). اما مقایسه تفاوت آنها بین گروه کنترل و درمان افراد نرموزوسپرمی و آزواسپرمی معنی&shy;دار نبود. همچنین مورفولوژی و کیفیت کروماتین اسپرم اختلاف معنی&shy;داری بین دو نمونه هر گروه وجود نداشت اگرچه کیفیت کروماتین نمونه&shy;های گروه درمان قبل و بعد از فریز بهتر از گروه کنترل بودند.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">علیرغم نتایج مثبتی که رسوراتول روی نمونه سیمن داشته نتوانست اثر قابل ملاحظه&shy;ای روی اسپرم شسته شده قبل و بعد فریز داشته باشد.</span></span><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"></span></span><br> &nbsp;<br> &nbsp; <strong>Background:</strong> Previous studies have examined the effect of resveratrol as a potent antioxidant for free radicals in semen. While, the prepared spermatozoa are more affected by ROS factors due to centrifugation and incubation.<br> <strong>Objective: </strong>To evaluate the RSV&#39;s effects on the prepared sperm parameters and chromatin quality in both normozoospermic and asthenozoospermic cases before and after freezing.<br> <strong>Materials and Methods:</strong> The sample of 10 normozoospermic and asthenozoospermic men was prepared through the swim-up method. The groups were then divided into two samples of control and experimental (exposure to 30 &micro;mol/l of RSV) to evaluate and compare the sperm parameters and chromatin quality before and after freezing.<br> <strong>Results: </strong>The motility and viability of spermatozoa were seen to be significantly different before and after freezing separately in the control and treatment samples of the groups (p &le; 0.001 and p = 0.001, respectively). However, the stated difference between the control and treatment samples of normozoospermic and asthenozoospermic patients were not significant (p > 0.05). In addition, the sperm morphology and chromatin quality were not significantly different between the two samples of each group; nonetheless, chromatin quality of the treated sample was better than that of the control before and after freezing.<br> <strong>Conclusion:</strong> Despite the protective effects of RSV on the semen samples, RSV cannot affect significantly the prepared sperms parameters and chromatin quality in normozoospermic and asthenozoospermic patients. رسوراتول, کروماتین, تحرک, اسپرم, فریز. Resveratrol, Chromatin, Motility, Spermatozoa, Freeze. 755 764 http://ijrm.ir/browse.php?a_code=A-10-473-1&slc_lang=en&sid=1 Masoomeh Mohammadzadeh m1978mm1355@gmail.com 0000-0003-2245-4700 No Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mohammad Ali Khalili khalili59@hotmail.com 0000-0001-9940-749x No Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Vahid Ramezani vahidramezani@rocketmail.com 0000-0003-4791-6182 No Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Hamed Hamishehkar hamishehkar.hamed@gmail.com 0000-0001-9905-0662 No Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Laleh Dehghan Marvast laleh.dm236@gmail.com 0000-0002-0708-3414 No Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Esmat Mangoli es.mangoli@gmail.com 0000-0001-6206-7831 No Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mahya Rajabi m.rajabi92@gmail.com 0000-0002-0122-9523 No Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Zhima Akhavan Sales Dr.zhima@live.com 0000-0002-7674-2620 No Department of Immunology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ali Reza Talebi prof_talebi@hotmail.com 0000-0003-4883-3020 Yes Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.