en بیان پروتئین فسفوریله بیضه‌ای در موش‌های با دیابت شیرین تیپ 1 و 2: یک مطالعه تجربی Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">دیابت شیرین تیپ 1 و 2 (</span></span><span dir="LTR"><span style="font-size:10.0pt;">DM</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) به عنوان علت اختلال در باروری یا ناباروری شناخته می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">شود. به هر حال مقایسه مارک</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های بالقوه در اسپرماتوژنر و استروئیدوژنز در مردان مبتلا به </span></span><span dir="LTR"><span style="font-size:10.0pt;">DM</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">انجام نشده است.</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">این مطالعه در نظر دارد که بیان پروتئین</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های فسفوریله تیروزین و تنظیم&shy;کننده مواد استروئیدوژنیک (</span></span><span dir="LTR"><span style="font-size:10.0pt;">StAR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) را در بیضه موش</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های </span></span><span dir="LTR"><span style="font-size:10.0pt;">DM</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بررسی کند.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش&shy;ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">56 موش نر </span></span><span dir="LTR"><span style="font-size:10.0pt;">C57BL/6</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> به چهار گروه تقسیم شدند (هر گروه 14 موش): گروه کنترل با دوزهای متعدد و پایین استرپتوزوسین (</span></span><span dir="LTR"><span style="font-size:10.0pt;">MLD-STZ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)، گروه </span></span><span dir="LTR"><span style="font-size:10.0pt;">MLD-STZ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> گروه کنترل رژیم با چربی بالا و </span></span><span dir="LTR"><span style="font-size:10.0pt;">(HFD-STZ) STZ</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و گروه </span></span><span dir="LTR"><span style="font-size:10.0pt;">HFD-STZ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، در گروه </span></span><span dir="LTR"><span style="font-size:10.0pt;">MLD-STZ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موش&shy;های با تیپ&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;</span></span><span dir="LTR"><span style="font-size:10.0pt;">DM</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> 2رژیم </span></span><span dir="LTR"><span style="font-size:10.0pt;">HFD</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">را برای 14 روز دریافت کردند و </span></span><span dir="LTR"><span style="font-size:10.0pt;">STZ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> به صورت </span></span><span dir="LTR"><span style="font-size:10.0pt;">i.p.</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">با دوز </span></span><span dir="LTR"><span style="font-size:10.0pt;">mg/kg</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> 85 تجویز شد و با </span></span><span dir="LTR"><span style="font-size:10.0pt;">HFD</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">تغذیه شدند. در انتهای مطالعه (روزهای 36 و 72) بیان پروتئین</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های فسفوریله و </span></span><span dir="LTR"><span style="font-size:10.0pt;">StAR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بررسی شد.</span></span><strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">پروتئین&shy;های فسفوریله تیروزین در انتهای اسپرماتیدها مایع لومینال و سلول&shy;های لیدیگ تجمع یافتند. تراکم فسفوریلاسیون 110، 85، 72، 60 و 52 </span></span><span dir="LTR"><span style="font-size:10.0pt;">KDas</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در موش</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های روز 36 کمتر بود. هر چند این تراکم در هر دو گروه مشاهده شد ولی فقط </span></span><span dir="LTR"><span style="font-size:10.0pt;">KDa</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">85 در موش</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های </span></span><span dir="LTR"><span style="font-size:10.0pt;">MLD-STZ</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بیشتر از موش&shy;های </span></span><span dir="LTR"><span style="font-size:10.0pt;">HDF</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در روز 72 بود. بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">StAR</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در هر دو گروه نسبت به کنترل</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها کاهش یافته بود.</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span><br> <strong><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری:</span></span></span></strong> <span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کاهش بیان </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">StAR</span></span> <span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و پروتئین</span></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;</span></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های فسفوریله تیروزین ممکن است که به صورت مستقیم درکاهش سطح تستوسترون و اختلال اسپرماتوژنز موثر باشد. این یافته&shy;ها پشتیبان فرضیه نقش هر دو نوع </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DM</span></span> <span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در ناباروری مردان می</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">&shy;</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باشد.</span></span></span> <strong>Background:</strong> Types 1 and 2 diabetes mellitus (DM) are known to be the cause of sub/infertility. However, the comparisons of potential markers in spermatogenesis and steroidogenesis in DM males have never been elucidated.<br> <strong>Objective:</strong> This study aimed to examine the expressions of tyrosine-phosphorylated and steroidogenic acute regulatory (StAR) proteins in testis of DM mice.<br> <strong>Materials and Methods:</strong> Fifty-six male C57BL/6 mice were divided into four groups (n = 14/ each): control of MLD-STZ (multiple low doses of streptozotocin), MLD-STZ, control of HFD-STZ (high-fat diet with STZ), and HFD-STZ. MLD-STZ mice (type 1 DM) were intraperitoneally (i.p.) injected with STZ at 40 mg/kg BW for five days. HFD-STZ mice (type 2 DM) received an HFD for 14 days and i.p.-induced by STZ at 85 mg/kg BW and fed with HFD. At the end of the experiment (days 36 and 72), the expressions of phosphorylated proteins and StAR were examined.<br> <strong>Results:</strong> Tyrosine phosphorylated proteins were localized in late spermatids, luminal fluid, and Leydig cells. The intensities of phosphorylated 110, 85, 72, 60, and 55 kDas were lower in the 36 day-DM mice. Although such intensities were present in both groups, only 85 kDa in the MLD-STZ mice was higher in HFD mice at 72 days. StAR expressions in both groups were decreased than that of the controls.<br> <strong>Conclusion:</strong> Decreased expressions of StAR and tyrosine-phosphorylated proteins may be directly involved in low testosterone levels and impaired spermatogenesis. These findings support the notion that both DM types play a role in male infertility. دیابت قندی, پروتئین فسفریله شده بیضه, پروتئین تنظیم‌کننده حاد استروئیدوژن, استرپتوزوتوسین, موش. Diabetes mellitus, Testicular phosphorylated protein, Steroidogenic acute regulatory protein, Streptozotocin, Mice. 567 576 http://ijrm.ir/browse.php?a_code=A-10-1-648&slc_lang=en&sid=1 Apichakan Sampannang 0000-0003-4929-6398 No Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Supatcharee Arun 0000-0002-7518-2406 No Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Jaturon Burawat 0000-0004-7856-5891 No Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Wannisa Sukhorum 0000-0003-3327-5636 No School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand Sitthichai Iamsaard sittia@kku.ac.th 0000-0002-6793-2879 Yes Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand