en اثر عصاره چای سبز بر پارامترهای اسپرم و تغییرات بافتی بیضه در رت‌های در معرض پارانونایل فنل Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">پارانونایل فنل (</span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) یک آلاینده زیست محیطی است که می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تواند رادیکال&shy;های آزاد را تولید و ویژگی&shy;های تولید&shy;مثلی را مختل کند. عصاره چای سبز (</span></span><span dir="LTR"><span style="font-size:10.0pt;">GTE</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) یک آنتی</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">اکسیدان است که ممکن است اثرات زیان</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آور رادیکال</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های آزاد را مهار کند.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از این مطالعه بررسی اثر عصاره چای سبز بر پارامترهای اسپرم و بافت بیضه در رت</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های تیمار شده با پارانونایل فنل بود.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موارد و روش &shy;ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">24 رت نر بالغ نژاد ویستار (20</span></span><span style="font-size:11.0pt;">&plusmn;</span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">215 گرم) به طور تصادفی به 4 گروه (6</span></span> <span dir="LTR"><span style="font-size:10.0pt;">n= </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در هر گروه) شامل کنترل،</span></span> <span dir="LTR"><span style="font-size:10.0pt;">mg/kg/day) </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">200)</span></span> <span dir="LTR"><span style="font-size:10.0pt;">mg/kg/day) p-NP </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">200</span></span> <span dir="LTR"><span style="font-size:10.0pt;">GTE (</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و</span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP+GTE </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;تقسیم شدند و به مدت 56 روز تیمار دهانی شدند. بیضه</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های سمت راست و اپیدیدیم سمت چپ برای بررسی پارامترهای مورد نظر استفاده گردید. ع</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">لاوه بر این، غلظت مالون دی</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آلدئید سرم نیز محاسبه شد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کاهش معنی</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">داری در تعداد، تحرک، قابلیت حیات و مورفولوژی اسپرم (001/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p< </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در رت</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های تیمار شده با </span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در مقایسه با گروه کنترل مشاهده شد. قطر لوله&shy;های سمی نفروس (001/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p< </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)، ضخامت اپیتلیوم زایشی (018/0 </span></span><span style="font-size:10.0pt;">=</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) حجم کل بیضه (009/0</span></span><span style="font-size:10.0pt;"> =</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و حجم لوله&shy;های سمی نفروس (001/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p< </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و وزن بیضه (017/0</span></span><span style="font-size:10.0pt;"> =</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در گروه </span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در مقایسه با سایر گروه</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها کاهش یافت. علاوه بر این افزایش معنی&shy;داری در غلظت مالون دی آلدئید در گروه </span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در مقایسه با کنترل دیده شد (043/0 </span></span><span style="font-size:10.0pt;">=</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). اکثر اثرات مخرب </span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">می<strong>&shy;</strong>تواند به دنبال مصرف</span></span> <span dir="LTR"><span style="font-size:10.0pt;">GTE</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">بهبود یابد.</span></span><span style="font-family:arial unicode ms,sans-serif;"><span style="font-size:11.0pt;"></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">به نظر می<strong>&shy;</strong>رسد</span></span> <span dir="LTR"><span style="font-size:10.0pt;">GTE</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">می</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تواند به عنوان یک آنتی</span></span><span dir="LTR"><span style="font-family:arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">اکسیدان قوی در مورد سمیت ناشی از </span></span><span dir="LTR"><span style="font-size:10.0pt;">p-NP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مورد استفاده قرار گیرد.</span></span> Abstract<br> <br> Background: Para-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals.<br> <br> Objective: The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats.<br> <br> Materials and Methods: 24 adult male Wistar rats (215 &plusmn; 20 gr) were randomly divided into four groups (n = 6/each) &ndash; including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE &ndash; and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated.<br> <br> Results: A significant decrease in the sperm number, motility, viability and morphology (p < 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p < 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p < 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE.<br> <br> Conclusion: It seems GTE can be used as a potent antioxidant in the case of p-NP toxication. عصاره چای سبز, پارانونایل فنل, اسپرم, بیضه, رت Green tea extract, Para-nonylphenol, Sperm, Testis, Rat. 717 726 http://ijrm.ir/browse.php?a_code=A-10-1-667&slc_lang=en&sid=1 Parisa Azizi 0000-0001-6266-3094 No Department of Biology, Faculty of Sciences, Arak University, Arak, Iran. Malek Soleimani Mehranjani m-Soleimani@araku.ac.ir 0000-0003-4822-2617 Yes Department of Biology, Faculty of Sciences, Arak University, Arak, Iran