en بررسی فراوانی و ارتباط پلی مورفیسم rs 14035 ژن RAN با سقط مکرر با علت ناشناخته: یک مقاله مورد-شاهدی Reproductive Genetics Original Article Original Article <pre dir="RTL" style="text-align:justify;text-justify:inter-ideograph;background:white;direction:rtl;unicode-bidi:embed;"> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">عوامل ژنتیکی می</span></span><span dir="LTR"><span style="font-family:inherit,serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">توانند باعث سقط مکرر ایدیوپاتیک شوند. ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">RAN</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">عضو &quot;خانواده بزرگ </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">RAS</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">&quot; و یک </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">GTPase</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کوچک است که برای انتقال </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">RNA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و پروتئین&shy;ها از طریق منافذ هسته&shy;ای ضروری است. جهش در ژن سازنده </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">RAN</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">می&shy;تواند سنتز </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">DNA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">را متوقف کند، و بیان ژن&shy;ها در رحم را تغییر دهد، </span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">احتمال می&shy;رود که در سقط مکرر نقش داشته باشد.</span></span></pre> <pre dir="RTL" style="text-align:justify;text-justify:inter-ideograph;background:white;direction:rtl;unicode-bidi:embed;"> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف از این مطالعه بررسی فراوانی پلی&shy;مورفیسم </span></span><span style="font-family:Times New Roman,serif;">(</span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">RAN</span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"> (rs 14035</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> در زنان مبتلا به سقط مکرر با علت ناشناخته در مقایسه با زنان بدون سابقه سقط جنین است.</span></span><span dir="LTR"><span style="font-family:inherit,serif;"><span style="font-size:11.0pt;"></span></span></span></pre> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها:</span></span></strong> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه مورد شاهدی، 100 زن 20 تا 40 ساله با حداقل دو مورد سقط جنین پیاپی قبل از هفته 20 بارداری و داشتن همسران با کاریوتایپ و پارامترهای نرمال اسپرم به عنوان گروه مورد و 100 زن بدون سابقه سقط جنین و داشتن حداقل یک بارداری موفق و زایمان طبیعی به عنوان گروه کنترل انتخاب شد. پلی&shy;مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">rs 14035</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> ژن </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">RAN</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">با استفاده از تکنیک </span></span><span dir="LTR"><span style="font-size:10.0pt;">(PCR-RFLP)</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> بررسی و فراوانی آن در دو گروه مقایسه گردید.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span> <pre dir="RTL" style="text-align:right;background:white;direction:rtl;unicode-bidi:embed;"> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">فراوانی ژنوتیپ&shy;های</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">TT</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">TC</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">CC</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> پلی</span></span><span dir="LTR"><span style="font-family:inherit,serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مورفیسم ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">RAN</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در گروه مورد به ترتیب 9% ، 40% و 51% بود و فراوانی در گروه شاهد به ترتیب 11%، 38% و 51% بود. </span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بین گروه مورد و شاهد اختلاف معنی&shy;داری از نظر ژنوتیپ&shy;ها وجود&nbsp; نداشت.</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> (882/0 </span></span><span style="font-family:Times New Roman,serif;">=</span><span dir="LTR"><span style="font-family:Times New Roman,serif;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">).</span></span></pre> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">با توجه به نتایج مطالعه حاضر به نظر می&shy;رسد پلی&shy;مورفیسم ژن </span></span><span style="font-size:10.0pt;">(</span><span dir="LTR"><span style="font-size:10.0pt;">rs 14035</span></span><span style="font-size:10.0pt;">) </span><em><span dir="LTR"><span style="font-size:10.0pt;">RAN</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">با خطر سقط مکرر خود</span></span><span dir="LTR"><span style="font-family:Courier New;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به</span></span><span dir="LTR"><span style="font-family:Courier New;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">خودی در جمعیت مورد مطالعه مرتبط نیست.</span></span> <strong>Background:</strong> Genetic factors could account for Recurrent pregnancy loss (RPL). The <em>RAN</em> gene is a member of the &quot;large RAS family&quot; and a small GTPase that is essential for the translocation of Ribonucleic acid (RNA) and proteins through the nuclear pore. Mutation in the <em>RAN</em> constitutive gene could stop DNA synthesis and alter the expression of genes in the uterus, likely playing a role in recurrent miscarriage<span dir="RTL">.</span><br> <strong>Objective:</strong> The aim was to investigate the frequency of <em>RAN</em> (rs 14035) polymorphism in women with RPL compared with women without abortion history<span dir="RTL">.</span><br> <strong>Materials and Methods:</strong> In this case-control study, 100 women with at least two consecutive miscarriages before the 20th wk of gestation and having spouses with karyotype and normal sperm parameters as the case group and 100 women with no history of abortion and having at least one successful pregnancy and normal delivery as the control group. The groups were age matched (20-40 yr). The rs 14035 polymorphism of <em>RAN</em> gene was investigated by Polymerase Chain Reaction-Restriction Fragment Length poly morphism technique and the frequency of which was compared between the two groups<span dir="RTL">.</span><br> <strong>Results:</strong> The frequency of TT, TC, and CC genotypes of <em>RAN</em> gene polymorphism in the case group were 9%, 40%, and 51%, respectively, and in the control group were 11%, 38%, and 51%, respectively. There was no significant difference in the genotypes between two groups (p = 0.882)<span dir="RTL">.</span><br> <strong>Conclusion:</strong> According to our results, it seems that <em>RAN</em> polymorphism (rs 14035) is not associated with the risk of RPL in this study population<span dir="RTL">.</span><br> &nbsp; ژن RAN, سقط مکرر, پلی مورفیسم, PCR-RFLP. RAN gene, Repeated abortion, Polymorphism, PCR-RFLP. 359 366 http://ijrm.ir/browse.php?a_code=A-10-35-3&slc_lang=en&sid=1 zahrasadat Mortazavifar nazanin_m2004@yahoo.com 0000-0002-1642-0021 No Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Hamidreza Ashrafzadeh hr_ashrafzadeh@yahoo.com 0000-0001-7138-9023 No Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Sayed Morteza Seifati seifati@gmail.com 0000-0002-8505-5267 No Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Nasrin Ghasemi n479g@yahoo.co.uk 0000-0002-1103-6004 Yes Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.