en تأثیر مکمل سریسین بر روی بلوغ، باروری و تکامل قبل از لانه گزینی جنین موش: مطالعه تجربی Assisted Reproductive Technologies Original Article Original Article <div style="text-align: justify;"><strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مراحل کشت رویان موش بخش مهمی از آزمایش&shy;های مطالعات زیست&shy;شناسی تراریخته، تولید&shy;مثل و تکامل است و محققین همواره به دنبال ارتقا محیط&shy;های کشت موجود هستند.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بررسی اثر سریسین به عنوان یک مکمل محیط کشت بر بلوغ، لقاح و تکامل آزمایشگاهی جنین موش.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه اثر سریسین را در سه غلظت: 1/0%، 5/0% و 1% به عنوان یک مکمل بر روی بلوغ آزمایشگاهی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">)، لقاح آزمایشگاهی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) و تکامل آزمایشگاهی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IVD</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) جنین</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های موش بررسی گردید. همچنین، به طور هم زمان اثر تجمعی سه غلظت 1/0، 5/0 و 1درصد از سریسین به عنوان مکمل از </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVD</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> مورد بررسی قرار گرفت.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج این مطالعه نشان داد که در گروه مطالعه </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، در مقایسه با گروه کنترل، تعداد اووسیست&shy;های رسیده به مرحله بلوغ </span></span><span dir="LTR"><span style="font-size:10.0pt;">MII</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به طور معنی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">داری در گروه 1% سریسین بیشتر بود. از طرف دیگر، نتایج قابل توجهی در گروه&shy;های </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVD</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">همراه با غلظت</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های مختلف سریسین نسبت به گروه کنترل مشاهده نشد. در آزمایش اثر سریسین از </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به</span></span><span dir="LTR"><span style="font-size:10.0pt;">IVD</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، تعداد تخمک&shy;ها پس از گذراندن </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVM</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و رسیدن به مرحله بلاستوسیست در غلظت 1% سرسین بیشتر از سایر گروها بود. همچنین در مقایسه با گروه کنترل تفاوت معنی&shy;داری مشاهده شد (048/0 </span></span><span dir="LTR"><span style="font-size:10.0pt;"><</span></span> <span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) با این حال، در گروه مطالعه </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVD</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هیچ ارتباط &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;معنی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">داری در غلظت&shy;های مختلف سریسین مشاهده نشد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری:</span></span></strong> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">غلظت 1% سریسین می&shy;تواند به عنوان یک مکمل در کشت جنین موش به ویژه برای بهبود میزان بلوغ آزمایشگاهی مورد استفاده قرار گیرد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong></div> <div style="text-align: justify;"><strong>Background:</strong> Mouse embryo culture condition is an essential part of transgenic, reproductive and developmental biology laboratories. Mouse embryonic culture media may have a high risk of serum contamination with pathogens.<br> <strong>Objective:</strong> To investigated the effect of sericin as an embryo culture medium supplement on in vitro maturation (IVM), in vitro fertilization (IVF), and development of the preimplantation embryo in mice.<br> <strong>Materials and Methods:</strong> The effects of sericin at three concentrations (subgroups) of 0.1%, 0.5%, and 1% as a medium supplement on IVM, IVF, and in vitro development of mouse embryos were separately investigated and compared with a sericin-free (control) group. The cumulative effect of the three concentrations was evaluated for IVM+ in vitro development and IVF+ in vitro development as follow-up groups.<br> <strong>Results:</strong> In the IVM group, compared to the control group, the number of oocysts reaching the MII stage was significantly higher when 1% sericin was used (161/208 = 77.4%). No significant results were observed in the IVF and in vitro development groups with different concentrations of sericin compared to the control group. Among the follow-up groups, in the IVM + in vitro development group, the number of oocytes was higher after passing the IVM and IVF and reaching the blastocysts stage when 1% sericin was used, compared with other sericin subgroups. A significant difference was also noted when compared with the control group (p = 0.048). The IVF+ in vitro development study group, on the other hand, did not show any significant relationship.<br> <strong>Conclusion:</strong> It can be concluded that 1% sericin can be used as a supplement in mouse embryo cultures to improve the IVM rate. Also, based on the findings, sericin appears to be an effective supplement which can have a positive effect on the development of embryos derived from IVM.</div> سریسین, بلوغ آزمایشگاهی, لقاح آزمایشگاهی, جنین قبل از لانه گزینی, محیط مکمل, موش. Sericin, In vitro maturation, In vitro fertilization, Preimplantation embryo, Culture medium, Mice. 921 928 http://ijrm.ir/browse.php?a_code=A-10-1329-1&slc_lang=en&sid=1 Omid Banafshi OmidBanafshi@gmail.com 0000-0001-5057-0878 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Sherko Nasseri sherko.naseri@yahoo.com 0000-0003-3113-7694 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Leila Farhadi LeilaFarhadi@gmail.com 0000-0002-9143-0019 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Masoud Alasvand Alasvand1100@gmail.com 0000-0002-0219-4203 No Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Mohammad Bagher Khadem-Erfan khademerfan@gmail.com 0000-0002-3101-2165 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Javad Hosseini Javad.705@gmail.com 0000-0002-5087-1424 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Saber Miraki saber.miraki@gmail.com 0000-0003-4278-8687 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Fardin Fathi farfath@gmail.com 0000-0002-4648-5598 Yes Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.