International Journal of Reproductive BioMedicine International Journal of Reproductive BioMedicine IJRM Medical Sciences http://ijrm.ir 1 admin 2476-4108 2476-3772 10.29252/ijrm en jalali 1400 1 1 gregorian 2021 4 1 19 4 online 1 fulltext
en افزایش ماتریکس متالوپروتئیناز -9 در ارتباط با اینترلوکین 17 و استرس روانی در مردان نابارور می باشد: یک مطالعه مقطعی Matrix metalloproteinase-9 is elevated and related to interleukin-17 and psychological stress in male infertility: A cross-sectional study Reproductive Andrology Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ماتریکس متالوپروتئیناز -9 (</span></span><span dir="LTR"><span style="font-size:10.0pt;">MMP-9</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">)، اینترلوکین -17 (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-17</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) و استرس روانی به عنوان عوامل موثر در پاتوژنز ناباروری مردان شناخته شده&shy;اند.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ارزیابی ارتباط </span></span><span dir="LTR"><span style="font-size:10.0pt;">MMP-9</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-17</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و استرس روانی در مردان نابارور.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش&shy; ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه مقطعی، 39 مرد مبتلا به ناباروری بر اساس آزمایش مایع منی و 39 نفر با نتیجه نرمال مایع منی شرکت داده شدند. </span></span><span dir="LTR"><span style="font-size:10.0pt;">MMP-9</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-17</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> در هر دو گروه توسط </span></span><span dir="LTR"><span style="font-size:10.0pt;">ELISA</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> برآورد شد. از آزمون مقیاس استرس ادراک شده برای ارزیابی استرس روانشناختی در هر دو گروه کنترل و مورد استفاده شد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در مردان با ناباروری، </span></span><span dir="LTR"><span style="font-size:10.0pt;">MMP-9</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-17</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در مقایسه با گروه شاهد به طور معنی&shy;داری افزایش یافت (به ترتیب 046/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 041/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">). ارتباط &nbsp;&nbsp;معنی&shy;داری بین </span></span><span dir="LTR"><span style="font-size:10.0pt;">MMP-9</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-17</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 037/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 335/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">r</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) و مقیاس استرس درک شده (329/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">r</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 041/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) مشاهده شد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری: </span></span></strong><span dir="LTR"><span style="font-size:10.0pt;">IL-17</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و استرس باعث افزایش میزان </span></span><span dir="LTR"><span style="font-size:10.0pt;">MMP-9</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> در مردان نابارور می&shy;شود.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> &nbsp;<br> &nbsp; <strong>Background: </strong>Matrix metalloproteinase-9 (MMP-9), interleukin-17 (IL-17) and psychological stress are known to play a role in the pathogenesis of male infertility.<br> <strong>Objective:</strong> To assess the association of MMP-9 with IL-17 and psychological stress in infertile men.<br> <strong>Materials and Methods:</strong> In this cross-sectional study, 39 men with infertility diagnosed based on semen analysis and 39 subjects with normal semen analysis were included in the study. MMP-9 and IL-17 were estimated in both groups by ELISA. Perceived stress scale was used to assess psychological stress in controls and cases.<br> <strong>Results: </strong>In infertile cases, MMP-9and IL-17 were significantly increased when compared with controls (p = 0.046, p = 0.041 respectively). A significant association of MMP-9 was observed with IL-17 (r = 0.335, p = 0.037) and perceived stress scale (r = 0.329, p = 0.041).<br> <strong>Conclusion:</strong> IL-17 and stress increase MMP- 9 levels in infertile men.<br> &nbsp; ناباروری, اینترلوکین­ها, پپتید هیدرولاز. Infertility, Interleukins, Peptide hydrolase. 333 338 http://ijrm.ir/browse.php?a_code=A-10-1061-2&slc_lang=en&sid=1 Ann Prasad Mary in980annpmary10@gmail.com 0000-0002-4724-7970 No Biochemistry Department, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India. Hanumanthappa Nandeesha nandijipmer@gmail.com 0000-0001-7152-1143 Yes Biochemistry Department, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India. Dasari Papa dasaripapa@gmail.com 0000-0003-1022-2750 No Department of Obstetrics and Gynecology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India. Thiagaraju Chitra drchitra@yahoo.com 0000-0002-3224-9180 No Department of Obstetrics and Gynecology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India. Rajesh Nachiappa Ganesh drngrajesh@gmail.com 0000-0002-2396-0739 No Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India. Vikas Menon drvmenon@gmail.com 0000-0001-8035-4658 No Department of Psychiatry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India.