International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1400
12
1
gregorian
2022
3
1
20
3
online
1
fulltext
en
بررسی غلظت اینترلوکین-3، 5 و 6 در مایع فولیکولی زنان نابارور مبتلا به آندومتریوز: یک مطالعه مقطعی
Evaluation of IL-3, IL-5, and IL-6 concentration in the follicular fluid of women with Endometriosis: A cross-sectional study
Reproductive Biology
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">آندومتریوز با پاسخ­های ایمونولوژیک غیر­طبیعی و شرایط التهابی و ضد­التهابی همراه است.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">این مطالعه با هدف بررسی غلضت مایع فولیکولی (</span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) در غلضت­های اینترلوکین -3، 5 و 6 در زنان مبتلا به اندومتریوز و زنان سالم انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مواد و روش­ ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در این مطالعه مقطعی 68 زن مراجعه­کننده به مرکز لقاح آزمایشگاهی بیمارستان امام رضا مشهد در سال 1397 به صورت تصادفی انتخاب شدند. سطح سیتوکینها در مایع فولیکولی نمونهها در گروه آندومتریوز و کنترل (هر کدام 34 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">n</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) بررسی شد. دقت تشخیصی سیتوکین­ها و ویژگی­های بالینی مورد ارزیابی قرار گرفت.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اینترلوکین-3 و اینترلوکین-6 در مایع فولیکولی زنان مبتلا به آندومتریوز نسبت به زنان عاری از بیماری تغییر معنی­داری نشان داد (به ترتیب 04/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> و 003/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">). میانگین غلظت اینترلئکین-5</span></span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در گروه آندومتریوز کمتر از گروه کنترل بود اما معنادار نبود (5/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">). همچنین اختلاف معنی­داری در چرخه قاعدگی، دیسپارونی و دیسمنوره بین دو گروه مشاهده شد (به ترتیب 01/0 </span></span><span lang="FA" style="font-size:10.0pt">></span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">، 04/0 </span></span><span dir="LTR" style="font-size:10.0pt">p =</span><span style="font-size:11.0pt"><span style="font-family:"B Mitra""> و 02/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">). دقت تشخیصی اینترلوکین-3 و اینترلوکین-6 در مایع فولیکولی بیماران کم و با سطح زیر منحنی به ترتیب 614/0 و 645/0 بود.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتیجه ­گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اگرچه هیچ کدام از سیتوکین­ها، ارزش پیش­بینی</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif"">­</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">کننده­ای برای آندومتریوز نداشتند، اما کاهش میزان اینترلوکین-3 و افزایش میزان اینترلوکین-6 در نمونه­های مایع فولیکولی بیماران مبتلا به آندومتریوز و همچنین عوامل خطرزایی از جمله چرخه قاعدگی نامنظم، دیسپارونی، و دیسمنوره می­تواند با پاتوژنز این بیماری دردناک در ارتباط باشد.</span></span><span lang="FA" style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
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<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Endometriosis is associated with abnormal immunologic responses and combined inflammatory and anti-inflammatory conditions. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objectives:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> This study aims to investigate follicular fluid (FF) concentration of interleukin (IL)-3, IL-5, and IL-6 in women with and without endometriosis. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> In this a<b> </b>cross-sectional<b> </b>study 68 women who were referred <span class="A0" minion="" pro="" style="font-family:"><span style="color:black"><span new="" roman="" style="font-family:" times="">to the in vitro fertilization center of Imam Reza hospital in Mashhad during 2018</span></span></span> was selected randomly. Leaves of cytokines in the FF samples were evaluated in the endometriosis and the control group (n = 34/each). The diagnostic accuracy of cytokines and clinical characteristics were evaluated.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> IL-3 and IL-6 were significantly changed in FF of the women with endometriosis compared with control group (p = 0.04, and p = 0.003, respectively), the mean concentration of IL-5 in the endometriosis group was lower than the control group (p = 0.5). But it was not significant. There were significant differences in the menstrual cycle, dyspareunia, and dysmenorrhea between the groups (p = 0.003, p = 0.04, and p = 0.02, respectively). The diagnostic accuracy of IL-3 and IL-6 in the FF was low, with the area under the curve of 0.614 and 0.645, respectively.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Although none of the cytokines </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">had a predictive value for endometriosis, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">the decreased levels of IL-3 and increased levels of IL-6 in the FF samples of women with endometriosis, and risk factors, including irregular menstrual cycle, dyspareunia, and dysmenorrhea, could be associated with the pathogenesis of this painful disease.</span></span></span></span></span></span></div>
اینترلوکین-3, اینترلوکین-5, اینترلوکین-6, مایع فولیکولی, آندومتریوز.
Interleukin-3, Interleukin-5, Interleukin-6, Follicular fluid, Endometriosis.
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http://ijrm.ir/browse.php?a_code=A-10-1352-1&slc_lang=en&sid=1
Mohammad
Ghodsi
mohammad.ghodsi62@yahoo.com