International Journal of Reproductive BioMedicine International Journal of Reproductive BioMedicine IJRM Medical Sciences http://ijrm.ir 1 admin 2476-4108 2476-3772 10.29252/ijrm en jalali 1400 3 1 gregorian 2021 6 1 19 6 online 1 fulltext
en بررسی ارتباط پلی مورفیسم های M55L و Q192R ژن آنزیم پارااکساناز 1 با خطر سقط مکرر در مطالعه مورد- شاهدی Association of M55L and Q192R polymorphisms of paraoxonase 1 gene (PON1) with recurrent pregnancy loss risk: A case-control study Fertility & Infertility Original Article Original Article <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">سقط مکرر (</span></span><span dir="LTR"><span style="font-size:10.0pt;">RPL</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) به شیوع 2 یا بیشتر سقط جنین قبل از نیمه اول بارداری اطلاق می</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">شود. فرضیه بر این است که استرس اکسیداتیو نقش اساسی در </span></span><span dir="LTR"><span style="font-size:10.0pt;">RPL</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">دارد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه ارتباط بین پلی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مورفیسم</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">های </span></span><span dir="LTR"><span style="font-size:10.0pt;">Q192R</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">L55M</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">آنزیم </span></span><span dir="LTR"><span style="font-size:10.0pt;">PON1</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به عنوان آنتی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">اکسیدانت و خطر </span></span><span dir="LTR"><span style="font-size:10.0pt;">RPL</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بررسی شد.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش &shy;ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در این مطالعه مورد شاهدی 110 فرد </span></span><span dir="LTR"><span style="font-size:10.0pt;">RPL</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و 110 بارور سالم وارد مطالعه شدند. </span></span><span dir="LTR"><span style="font-size:10.0pt;">DNA</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">محیطی از خون محیطی در تمام افراد استخراج شد. پلی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مورفیسم</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ها به روش </span></span><span dir="LTR"><span style="font-size:10.0pt;">RFLP-PCR</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> ژنوتیپ شدند. آنالیز آماری با استفاده از نرم افزار </span></span><span dir="LTR"><span style="font-size:10.0pt;">SPSS v16</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> انجام شد. و محاسبات معنی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">داری بین ژنوتیپ</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ها و فراوانی آن</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ها بوسیله تست کای اسکوار انجام شد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">آنالیز آماری پلی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">Q192R</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> اختلاف معنی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">داری را برای ژنوتیپ </span></span><span dir="LTR"><span style="font-size:10.0pt;">RR</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بین گروه مورد و شاهد نشان داد (005/0 </span></span><span style="font-size:10.0pt;">=</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 87/86-39/1 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">CI</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">،&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;11 </span></span><span style="font-size:10.0pt;">=</span><span dir="LTR"><span style="font-size:10.0pt;">OR</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) اما برای ژنوتیپ </span></span><span dir="LTR"><span style="font-size:10.0pt;">QR</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">QQ</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">ارتباط معنی&shy;دار دیده نشد. همچنین هیچ ارتباط معنی&shy;داری بین فراوانی آلل&shy;های </span></span><span dir="LTR"><span style="font-size:10.0pt;">R</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Q</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مشاهده نگردید (53/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">). آنالیز آماری پلی&shy;مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">L55M</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">برای ژنوتیپ </span></span><span dir="LTR"><span style="font-size:10.0pt;">MM</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در گروه مورد در مقایسه با گروه شاهد ارتباط معنی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">داری نشان داد (042/0 </span></span><span style="font-size:10.0pt;">=</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 30/13-97/0 </span></span><span style="font-size:10.0pt;">= </span><span dir="LTR"><span style="font-size:10.0pt;">CI</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، 59/3 </span></span><span style="font-size:10.0pt;">=</span><span dir="LTR"><span style="font-size:10.0pt;">OR</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) اما درمورد </span></span><span dir="LTR"><span style="font-size:10.0pt;">LM</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">LL</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">این ارتباط دیده نشد.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه &shy;گیری: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج این مطالعه ارتباط معنی&shy;دار بین پلی&shy;مورفیسم&shy;های </span></span><span dir="LTR"><span style="font-size:10.0pt;">Q192R</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">L55M</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> آنزیم </span></span><span dir="LTR"><span style="font-size:10.0pt;">PON1</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> با </span></span><span dir="LTR"><span style="font-size:10.0pt;">RPL</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> نشان داد.</span></span><br> &nbsp;<br> <strong><em><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">کلمات کلیدی: </span></span></em></strong><em><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">بارداری، سقط، آنزیم پارااکساناز 1، پلی&shy;مورفیسم، سقط مکرر.</span></span></em><strong><em><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:10.0pt;"></span></span></span></em></strong> <div style="text-align: justify;"><strong>Background:</strong> Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL.<br> <strong>Objective:</strong> To investigate the relationship between Q192R and L55M polymorphisms of <em>PON1</em> as antioxidant enzyme and the risk of RPL.<br> <strong>Materials and Methods: </strong>In this case-control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method.<br> <strong>Results: </strong>Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39-86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic frequency in the RPL participants compared to the control group (p = 0.53). Also, statistical analysis of the L55M polymorphism for MM genotype in the case group compared with the control group showed a significant difference (OR = 3.59, CI = 0.97-13.30, p = 0.042), but none for the LM and LL genotypes.<br> <strong>Conclusion: </strong>The findings showed a significant correlation between the Q192R polymorphisms and the L55M <em>PON1</em> enzyme and RPL in this study population.</div> بارداری, سقط, آنزیم پارااکساناز 1, پلی­مورفیسم, سقط مکرر. Pregnancy, Abortion, PON1, Polymorphism, Recurrent pregnancy loss. 559 568 http://ijrm.ir/browse.php?a_code=A-10-1405-1&slc_lang=en&sid=1 Mehdi Alizadeh mehdialz99@gmail.com 0000-0003-4306-5480 No International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mahboobeh Nasiri nasiri@iaua.ac.ir 0000-0003-1370-6849 No Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran. Morteza Samadi samadi@ssu.ac.ir 0000-0002-9220-6174 No Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Nasrin ghasemi ghasemi@ssu.ac.ir 0000-0002-1103-6004 No Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ali Moradi morady20018@gmail.com 0000-0002-2103-2592 Yes Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.