en بررسی ارتباط بین پلی مورفیسم تک نوکلئوتیدی C158T ژن PTPN22 با سقط مکرر با دلایل ناشناخته: یک مطالعه مورد-شاهدی Cellular and Molecular Biology of Reproduction Original Article Original Article <div style="text-align: justify;"><strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">آنزیم تیروزین فسفاتاز لنفوئیدی که توسط ژن </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">PTPN22</span></span></em> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">کد می‌شود، از طریق دفسفیلاسیون مولکول‌های میانجی‌گر سیگنال&shy;دهی در سلول‌های ایمنی، نقش مهمی در تنظیم پاسخ ایمنی ایفا می‌کند.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">به دلیل اهمیت پاسخ ایمنی متعادل در طی بارداری، هدف مطالعه حاضر مقایسه‌ فراوانی پلی‌مورفیسم </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">PTPN22</span></span></em><span dir="LTR"><span style="font-size:10.0pt;"> C1858T</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">بین افراد با سابقه سقط مکرر بدون علت (</span></span><span dir="LTR"><span style="font-size:10.0pt;">URPL</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) و گروه شاهد، برای اولین بار بوده است.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مواد و روش&shy; ها: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">در مرکز ناباروری یزد، ژنوم 200 فرد سقط مکرر و 200 فرد سالم (گروه کنترل)، به روش رسوبی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">Salting out</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">) جدا شدند، پلی‌مورفیسم </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">PTPN22</span></span></em><span dir="LTR"><span style="font-size:10.0pt;"> C1858T</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> در دو گروه به روش </span></span><span dir="LTR"><span style="font-size:10.0pt;">PCR&ndash;RFLP</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> بررسی شد، فراوانی ژنوتایپ‌ها در بانوان سقط مکرر و گروه کنترل بارور توسط آزمون آماری&nbsp;&nbsp;&nbsp; &nbsp;</span></span><span dir="LTR"><span style="font-size:10.0pt;">Chi-square</span></span> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مقایسه شدند.</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتایج نشان داد که فراوانی پلی‌مورفیسم </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">PTPN22</span></span></em><span dir="LTR"><span style="font-size:10.0pt;"> C1858T</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">، بین افراد با سقط مکرر و گروه کنترل بارور و متفاوت بوده است 32% و 5/21% &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(01/0</span></span><span dir="LTR"><span style="font-size:10.0pt;">p = </span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">).</span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">نتیجه&shy; گیری:</span></span></strong> <span style="font-family:B Mitra;"><span style="font-size:11.0pt;">یافته‌های ما پیشنهاد می‌دهد که پلی‌مورفیسم </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">PTPN22</span></span></em><span dir="LTR"><span style="font-size:10.0pt;"> 1858T</span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;"> نقش احتمالی در سقط مکرر دارد بنابراین تعیین ژنوتایپ پلی</span></span><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;">&shy;</span></span></span><span style="font-family:B Mitra;"><span style="font-size:11.0pt;">مورفیسم ذکر شده می‌تواند به کادر بالین در پیش‌بینی خطر احتمالی سقط مکرر کمک کند.</span></span><strong><span dir="LTR"><span style="font-family:Arial,sans-serif;"><span style="font-size:11.0pt;"></span></span></span></strong></div> <div style="text-align: justify;"><strong>Background:</strong> Lymphoid-tyrosine-phosphatase which is encoded by the protein tyrosine phosphatase non-receptor 22 (<em>PTPN22</em>) gene plays a pivotal role in the regulation of immune responses by dephosphorylating several signaling intermediates of immune cells.<br> <strong>Objective:</strong> Since a balanced immune response has been shown to be important during pregnancy, the purpose of this research was to compare the frequency of the <em>PTPN22</em> C1858T polymorphism in women with unexplained recurrent pregnancy loss (URPL) vs. in a control group for the first time.<br> <strong>Materials and Methods:</strong> Genomic DNA from 200 individuals with URPL and 200 individuals without URPL (the control group) at the infertility center in Yazd, Iran was isolated using the salting-out method. The <em>PTPN22</em> C1858T polymorphism of the two groups was analyzed using polymerase chain reaction-restriction fragment length polymorphism. Genotype frequencies in the women with URPL and the fertile control group were compared using the Chi-square test.<br> <strong>Results:</strong> There were significant differences in the frequency of the <em>PTPN22</em> 1858T polymorphism in the URPL individuals vs. the healthy controls, i.e. 32.0% and 21.5%, respectively (p = 0.01).<br> <strong>Conclusion: </strong>Our findings suggest that the <em>PTPN22</em> 1858T polymorphism could play a role in recurrent pregnancy loss. Therefore, genotyping of the mentioned polymorphism can help clinicians to predict the probable risk of URPL.</div> سقط مکرر, پروتئین PTPN22, پلی‌مورفیسم تک‌نوکلئوتیدی. Recurrent pregnancy loss, PTPN22 protein, Single nucleotide polymorphism. 873 880 http://ijrm.ir/browse.php?a_code=A-10-1453-1&slc_lang=en&sid=1 Fateme Khanbarari fatemeh.khanbarari@gmail.com 0000-0003-1960-5002 No Immunology Department, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Nasrin Ghasemi nghasemi479@gmail.com 0000-0002-1103-6004 No Abortion Research Center, Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mahmood Vakili Vakilim52@yahoo.com 0000-0002-0113-7100 No Health Monitoring Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Morteza Samadi samadi.for@gmail.com 0000-0002-9220-6174 Yes Abortion Research Center, Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.