en تشخیص اوره‌آپلاسما اوره لیتیکوم و مایکوپلاسما ژنیتالیوم و کیفیت اسپرم: یک مطالعه مقطعی در ویتنام Reproductive Andrology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اوره‌آپلاسما اوره‌آلیتیکوم (</span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و مایکوپلاسما ژنیتالیوم (</span></span><i><span dir="LTR" style="font-size:10.0pt">M. genitalium</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) ممکن است دستگاه تناسلی مرد را کلونیزه کنند. با این حال، اثرات منفی این باکتری</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها بر کیفیت کلی اسپرم، از جمله </span></span><span dir="LTR" style="font-size:10.0pt">pH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> مایع منی، غلظت اسپرم، تحرک، مورفولوژی و تعداد کل اسپرم نامشخص است.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه با هدف تعیین حضور </span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">M. genitalium</span></i><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تناسلی در مایع منی و بررسی تأثیر این ارگانیسم&shy;ها بر کیفیت اسپرم انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یک مطالعه مقطعی بر روی 380 مرد از زوج&shy;های نابارور در یک بیمارستان دانشگاهی سطح سه از جولای 2017 تا ژوئن 2018 انجام شد. کیفیت مایع منی بر اساس استاندارد 2010 سازمان بهداشت جهانی تجزیه و تحلیل شد و </span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">M. genitalium</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در نمونه&shy;های منی با استفاده از واکنش زنجیره&shy;ای پلیمراز شناسایی شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">338 مرد (9/88%) با حداقل یک پارامتر منی غیرطبیعی مراجعه کردند. میزان تشخیص </span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">M. genitalium</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به ترتیب 05/16 و 79/0% بود. بین گروه </span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> مثبت و گروه </span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> منفی از نظر خصوصیات اسپرم تفاوت معنی&shy;داری وجود نداشت. تحرک اسپرم و سرزندگی اسپرم در گروه مایکوپلاسما مثبت بسیار کمتر از گروه مایکوپلاسما منفی بود (به ترتیب 02/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و 001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">وجود </span></span><i><span dir="LTR" style="font-size:10.0pt">U. urealyticum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در منی مردان نابارور تأثیری بر ویژگی‌های اسپرم نداشت. اگرچه میزان مثبت </span></span><i><span dir="LTR" style="font-size:10.0pt">M. genitalium</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> کم بود، کلونیزاسیون توسط این باکتری‌ها به نظر می&shy;رسد بیشتر بر کیفیت اسپرم تأثیر منفی داشته باشد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:10.0pt"></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span new="" roman="" style="font-family:" times=""><b>Background:</b> <i>Ureaplasma urealyticum</i> (<i>U. urealyticum</i>) and <i>Mycoplasma genitalium</i> (<i>M. genitalium</i>) may colonize the male genital tract. However, the negative effects of these bacteria on overall sperm quality, including semen pH, sperm concentration, motility, morphology, and total sperm count remain unclear.</span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span new="" roman="" style="font-family:" times=""><b>Objective:</b> This study aimed to determine the presence of genital <i>U. urealyticum</i> and <i>M. genitalium</i> in semen and evaluate the effect of these organisms on sperm quality.</span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span new="" roman="" style="font-family:" times=""><b>Materials and Methods:</b> A cross-sectional study was conducted on 380 men from infertile couples at a tertiary university hospital from July 2017 to June 2018. Semen quality was analyzed according to the World Health Organization 2010 standard, and <i>U. urealyticum</i> and <i>M. genitalium</i> were detected in the semen samples using polymerase chain reaction.</span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span new="" roman="" style="font-family:" times=""><b>Results:</b> 338 men (88.9%) presented with at least one abnormal semen parameter. The detection rates of <i>U. urealyticum</i> and <i>M. genitalium</i> were 16.05% and 0.79%, respectively. There was no significant difference between the <i>Ureaplasma</i>-positive group and the <i>Ureaplasma</i>-negative group in terms of sperm characteristics. Sperm motility and sperm vitality in the <i>Mycoplasma-</i>positive group were much lower than those in the <i>Mycoplasma</i>-negative group (p = 0.02 and p < 0.001, respectively).</span></span></span></div> <h1 style="text-align: justify;"><span style="font-size:11pt"><span sans-serif="" style="font-family:Arial,"><span style="color:black"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:normal">The presence of <i>U. urealyticum</i> in the semen of infertile men did not affect the sperm characteristics. Although the positive rate of <i>M. genitalium</i> was low, colonization by these bacteria was more likely to negatively affect sperm quality. </span></span></span></span></span></span></h1> اوره‌آپلاسما اوره‌آلیتیکوم, مایکوپلاسما ژنیتالیوم, ناباروری, اسپرم. Ureaplasma urealyticum, Mycoplasma genitalium, Infertility, Spermatozoa. 185 194 http://ijrm.ir/browse.php?a_code=A-10-1567-1&slc_lang=en&sid=1 Minh Tam Le leminhtam@huemed-univ.edu.vn 0000-0001-6225-3108 Yes Center for Reproductive Endocrinology and Infertility, Hue University Hospital, Hue University, Hue, Vietnam. Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Dac Nguyen Nguyen ndnguyen@huemed-univ.edu.vn 0000-0002-0406-1711 No Center for Reproductive Endocrinology and Infertility, Hue University Hospital, Hue University, Hue, Vietnam. Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Hoang Bach Nguyen nhbach@huemed-univ.edu.vn 0000-0002-5391-2172 No Department of Microbiology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Viet Quynh Tram Ngo nvqtram@huemed-univ.edu.vn 0000-0003-3117-2756 No Department of Microbiology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Vu Quoc Huy Nguyen nvqhuy@huemed-univ.edu.vn 0000-0002-4744-7059 No Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.