en عصاره میوه های Basella alba، Grewia asiatica، Solanum nigrum و Ficus carica هیستوپاتولوژی بیضه ناشی از قرار گرفتن در معرض تتراکلرید کربن در موش‌های آلبینو را بهبود می‌بخشد: یک مطالعه تجربی Reproductive Biology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تتراکلرید کربن (</span></span><span dir="LTR" style="font-size:10.0pt">CCl₄</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) به</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">طور گسترده در صنایع مختلف استفاده می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود و باعث ایجاد استرس اکسیداتیو در انسان می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود. متأسفانه، این ماده به عنوان یکی از سموم قوی موثر در تولید</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مثل مردان نادیده گرفته شده است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پژوهش حاضر هیستوپاتولوژی بیضه ناشی از </span></span><span dir="LTR" style="font-size:10.0pt">CCl₄</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و بهبودهای حاصل از چهار عصاره پالپ میوه&shy;های دارویی (</span></span><span dir="LTR" style="font-size:10.0pt">FPEs</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) را در موش گزارش می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">60 موش نر آلبینو به شش گروه (10/گروه) به شرح زیر تقسیم شدند: </span></span><span dir="LTR" style="font-size:10.0pt">a</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) گروه کنترل (</span></span><span dir="LTR" style="font-size:10.0pt">Vc</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، </span></span><span dir="LTR" style="font-size:10.0pt">b</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) </span></span><span dir="LTR" style="font-size:10.0pt">,CCl₄ (C)</span> <span dir="LTR" style="font-size:10.0pt">c-f</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;</span></span><span dir="LTR" style="font-size:10.0pt">CCl₄+<i>Basella alba</i> (CBa), CCl₄+<i>Solanum nigrum</i> (CSn), CCl₄+<i>Ficus carica</i> (CFc)</span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">,</span></span><span dir="LTR" style="font-size:10.0pt"> CCl₄+<i>Grewia asiatica</i> (CGa)</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">. به استثنای گروه کنترل، </span></span><span dir="LTR" style="font-size:10.0pt">CCl₄</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (1/0 میلی‌لیتر از 2/0 میلی‌لیتر بر کیلوگرم) در روغن ذرت به حیوانات داده شد. چهار عصاره گیاهی (هر کدام 1/0 میلی&shy;لیتر) به ترتیب به حیوانات گروه </span></span><span dir="LTR" style="font-size:10.0pt">FPE</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مربوطه برای پنج روز متوالی بعدی داده شد، در حالی که حیوانات در گروه&shy;های </span></span><span dir="LTR" style="font-size:10.0pt">Vc</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span dir="LTR" style="font-size:10.0pt">CCl₄</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به جای </span></span><span dir="LTR" style="font-size:10.0pt">FPE</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آب دریافت کردند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">قرار گرفتن در معرض </span></span><span dir="LTR" style="font-size:10.0pt">CCl₄</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> منجر به تغییرات هیستومتری و بافت</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شناسی مختلف (از دست دادن بافت بینابینی و انواع اسپرماتیدهای بدون دم با سر بزرگ شده) شد که در همه موش</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها به جز </span></span><i><span dir="LTR" style="font-size:10.0pt">Solanum nigrum</span></i><span dir="LTR" style="font-size:10.0pt"> FPE</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> پس از درمان این تغییرات بهبود داده شد. داده‌های میکرومتریک برش‌های بیضه نیز کاهش قابل‌توجهی در تعداد اسپرماتوگونی را نشان داد، در حالی که سطح مقطع سر اسپرم‌ها در گروه‌های </span></span><span dir="LTR" style="font-size:10.0pt">CSn</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span dir="LTR" style="font-size:10.0pt">C</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به طور قابل‌ توجهی بالاتر بود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به جز </span></span><i><span dir="LTR" style="font-size:10.0pt">Solanum nigrum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، سه </span></span><span dir="LTR" style="font-size:10.0pt">FPE</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> دیگر به‌ویژه </span></span><i><span dir="LTR" style="font-size:10.0pt">Ficus carica</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، خواص توانبخشی در برابر تغییرات مرتبط با مواجهه با </span></span><span dir="LTR" style="font-size:10.0pt">CCl₄</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در هیستوپاتولوژی‌های بیضه از خود نشان دادند.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><strong>Abstract</strong><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Background: </b>Carbon tetrachloride (CCl₄) is extensively used in various industries and induces oxidative stress in humans. Unfortunately, it is one of the neglected potent male reproductive toxicants.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Objective: </b>The present research reports the testicular histopathology of CCl₄ and ameliorations by four medicinal fruit pulp extracts (FPEs) in mice.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Materials and Methods: </b>Sixty male albino mice were divided into six groups (10/group) as per the following: 1. Vehicle control (Vc); 2. CCl₄ (C); 3-6. CCl₄+<i>Basella alba</i> (CBa), CCl₄+<i>Solanum nigrum</i> (CSn), CCl₄+<i>Ficus carica</i> (CFc) and CCl₄+<i>Grewia asiatica</i> (CGa). Except for the control group, CCl₄(0.1 mL of 0.2 mL kg^-1) was given to the animals in corn oil. The four plant extracts (0.1 mL each) were respectively given to the relevant FPE group animals for the next five consecutive days, while the animals in the Vc and CCl₄ groups received water instead of FPE.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Results: </b>The CCl₄ exposure led to various histometric and histological alterations (loss of interstitial tissue and various dislodged tailless spermatids with enlarged heads) that were recovered in all except <i>Solanum nigrum </i>FPE mice post-treatment. The micrometric data of testicular sections also indicated significant decline in the number of spermatogonia, while the cross-sectional area of the sperm heads remained significantly higher in the CSn and C groups.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Conclusion: </b>Except for <i>Solanum nigrum</i>,<i> </i>the three FPEs, especially <i>Ficus carica</i>,<i> </i>showed rehabilitative properties against CCl₄ exposure-related modifications in testicular histopathologies.</span></span></div> Basella alba, Grewia asiatica, Solanum nigrum, Ficus carica, بیضه. Basella alba, Grewia asiatica, Solanum nigrum, Ficus carica, testis. 101 110 http://ijrm.ir/browse.php?a_code=A-10-1615-1&slc_lang=en&sid=1 Syeda Nadia Amad rehmanatta111@gmail.com 0000-0002-6395-806X No Government Associate College for Women Bhera, District Sargodha, Pakistan. Khawaja Raees Ahmad Ahmad k.r.ahmad@gmail.com 0000-0002-2408-9869 Yes Department of Zoology, University of Sargodha, District Sargodha, Pakistan. Usma Abdullah Usma Asmaniazi99@gmail.com 0000-0001-5225-7834 No Government Degree College for Women Mianwali, Sargodha, Pakistan. Fiza Malik Fiza fizamalik4140@gmail.com 0000-0002-4429-5619 No Department of Zoology, University of Sargodha, District Sargodha, Pakistan. Rabiyah Ali alirabiyah@gmail.com 0000-0002-0541-278X No Department of Zoology, University of Sargodha, District Sargodha, Pakistan. Umara Amir-ud-din umaraharoon68@gmail.com 0000-0003-4225-9953 No Department of Zoology, University of Sargodha, District Sargodha, Pakistan. Muhammad Ali Kanwal muhammadalidr@yahoo.com 0000-0001-5344-3620 No Department of Zoology, University of Sargodha, District Sargodha, Pakistan. Iram Inayat iram.inayat@hotmail.com 0000-0001-7041-6296 No Department of Zoology, University of Sargodha, District Sargodha, Pakistan.